Nanozyme-Mediated Catalytic Signal Amplification for Microfluidic Biosensing of Foodborne Bacteria.
Anal Chem
; 95(35): 13391-13399, 2023 09 05.
Article
en En
| MEDLINE
| ID: mdl-37610722
ABSTRACT
Early detection of foodborne bacteria is urgently needed to ensure food quality and to avoid the outbreak of foodborne bacterial diseases. Here, a kind of metal-organic framework (Zr-MOF) modified with Pt nanoparticles (Pt-PCN-224) was designed as a peroxidase-like signal amplifier for microfluidic biosensing of foodborne bacteria. Taking Escherichia coli (E. coli) O157H7 as a model, a linear range from 2.93 × 102 to 2.93 × 108 CFU/mL and a limit of detection of 2 CFU/mL were obtained. The whole detection procedure was integrated into a single microfluidic chip. Water, milk, and cabbage samples were successfully detected, showing consistency with the results of the standard culture method. Recoveries were in the range from 90 to 110% in spiked testing. The proposed microfluidic biosensor realized the specific and sensitive detection of E. coli O157H7 within 1 h, implying broad prospects of MOF with biomimetic enzyme activities for biosensing.
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Escherichia coli O157
/
Enfermedades Transmitidas por los Alimentos
Tipo de estudio:
Screening_studies
Límite:
Humans
Idioma:
En
Año:
2023
Tipo del documento:
Article