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Application of specific ELISAs for BMP15 and GDF9 to cumulus cell extracts from infertile women.
Krysta-Matter, A E; Riepsamen, A H; Lien, S; Wong, W Y T; Richani, D; Kilani, S; Harrison, C A; Mallitt, K A; Ledger, W L; Robertson, D M; Gilchrist, R B.
Afiliación
  • Krysta-Matter AE; Fertility & Research Centre, Discipline of Women's Health, School of Clinical Medicine, University of New South Wales Sydney, New South Wales, 2052, Australia; IVF Australia, Virtus Health, Alexandria, New South Wales, 2035, Australia.
  • Riepsamen AH; Fertility & Research Centre, Discipline of Women's Health, School of Clinical Medicine, University of New South Wales Sydney, New South Wales, 2052, Australia.
  • Lien S; Fertility & Research Centre, Discipline of Women's Health, School of Clinical Medicine, University of New South Wales Sydney, New South Wales, 2052, Australia.
  • Wong WYT; Fertility & Research Centre, Discipline of Women's Health, School of Clinical Medicine, University of New South Wales Sydney, New South Wales, 2052, Australia.
  • Richani D; Fertility & Research Centre, Discipline of Women's Health, School of Clinical Medicine, University of New South Wales Sydney, New South Wales, 2052, Australia.
  • Kilani S; Fertility & Research Centre, Discipline of Women's Health, School of Clinical Medicine, University of New South Wales Sydney, New South Wales, 2052, Australia; IVF Australia, Virtus Health, Alexandria, New South Wales, 2035, Australia.
  • Harrison CA; Monash Biomedicine Discovery Institute, Monash University, Victoria, 3800, Australia.
  • Mallitt KA; Fertility & Research Centre, Discipline of Women's Health, School of Clinical Medicine, University of New South Wales Sydney, New South Wales, 2052, Australia; Centre for Big Data Research in Health, University of New South Wales Sydney, New South Wales, 2052, Australia.
  • Ledger WL; Fertility & Research Centre, Discipline of Women's Health, School of Clinical Medicine, University of New South Wales Sydney, New South Wales, 2052, Australia; IVF Australia, Virtus Health, Alexandria, New South Wales, 2035, Australia.
  • Robertson DM; Fertility & Research Centre, Discipline of Women's Health, School of Clinical Medicine, University of New South Wales Sydney, New South Wales, 2052, Australia.
  • Gilchrist RB; Fertility & Research Centre, Discipline of Women's Health, School of Clinical Medicine, University of New South Wales Sydney, New South Wales, 2052, Australia. Electronic address: r.gilchrist@unsw.edu.au.
Mol Cell Endocrinol ; 578: 112049, 2023 Dec 01.
Article en En | MEDLINE | ID: mdl-37666445
ABSTRACT
Bone morphogenetic protein 15 (BMP15) and growth differentiation factor 9 (GDF9) are oocyte-specific paracrine factors which regulate ovarian cumulus cell (CC) functions. This study aimed to investigate if BMP15 and GDF9 bound to CCs can be characterized, quantified, and show an association with IVF outcomes in infertile women. BMP15 and GDF9 ELISAs were validated and applied to discarded CC extracts. Pooled CCs from individual patients were collected from 120 (cohort 1; BMP15 only) and 81 infertility patients (cohort 2; BMP15 and GDF9) undergoing superovulation. BMP15 and GDF9 levels expressed per CC DNA were correlated with maternal age, clinical and embryology data. Total BMP15 and GDF9 were highly correlated with each other (r = 0.9, p < 0.001). The GDF9BMP15 ratio was unrelated to oocyte number or age. BMP15/CC DNA and GDF9/CC DNA were unaffected by the type of superovulation and were not related to oocyte/embryo outcomes.
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Texto completo: 1 Banco de datos: MEDLINE Idioma: En Año: 2023 Tipo del documento: Article
Texto completo
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Texto completo: 1 Banco de datos: MEDLINE Idioma: En Año: 2023 Tipo del documento: Article