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A versatile pumpless multi-channel fluidics system for maintenance and real-time functional assessment of tissue and cells.
Kamat, Varun; Grumbine, Matthew K; Bao, Khang; Mokate, Kedar; Khalil, Gamal; Cook, Daniel; Clearwater, Brandon; Hirst, Richard; Harman, Jarrod; Boeck, Myriam; Fu, Zhongjie; Smith, Lois E H; Goswami, Moloy; Wubben, Thomas J; Walker, Emily M; Zhu, Jie; Soleimanpour, Scott A; Scarlett, Jarrad M; Robbings, Brian M; Hass, Daniel; Hurley, James B; Sweet, Ian R.
Afiliación
  • Kamat V; University of Washington Medicine Diabetes Institute, University of Washington, Seattle, WA 98109, USA.
  • Grumbine MK; EnTox Sciences, Inc., Mercer Island, WA 98040, USA.
  • Bao K; EnTox Sciences, Inc., Mercer Island, WA 98040, USA.
  • Mokate K; University of Washington Medicine Diabetes Institute, University of Washington, Seattle, WA 98109, USA.
  • Khalil G; EnTox Sciences, Inc., Mercer Island, WA 98040, USA.
  • Cook D; EnTox Sciences, Inc., Mercer Island, WA 98040, USA.
  • Clearwater B; Technical Assembly Service Corporation, Seattle, WA 98109, USA.
  • Hirst R; Technical Assembly Service Corporation, Seattle, WA 98109, USA.
  • Harman J; Department of Ophthalmology, Boston Children's Hospital, Harvard Medical School, Boston, MA 02115, USA.
  • Boeck M; Department of Ophthalmology, Boston Children's Hospital, Harvard Medical School, Boston, MA 02115, USA; Eye Center, Medical Center, Faculty of Medicine, University of Freiburg, Freiburg im Breisgau, Germany.
  • Fu Z; Department of Ophthalmology, Boston Children's Hospital, Harvard Medical School, Boston, MA 02115, USA.
  • Smith LEH; Department of Ophthalmology, Boston Children's Hospital, Harvard Medical School, Boston, MA 02115, USA.
  • Goswami M; Department of Ophthalmology and Visual Sciences, University of Michigan, Ann Arbor, MI 48105, USA.
  • Wubben TJ; Department of Ophthalmology and Visual Sciences, University of Michigan, Ann Arbor, MI 48105, USA.
  • Walker EM; Department of Internal Medicine, University of Michigan, Ann Arbor, MI 98195, USA.
  • Zhu J; Department of Internal Medicine, University of Michigan, Ann Arbor, MI 98195, USA.
  • Soleimanpour SA; Department of Internal Medicine, University of Michigan, Ann Arbor, MI 98195, USA.
  • Scarlett JM; University of Washington Medicine Diabetes Institute, University of Washington, Seattle, WA 98109, USA; Department of Pediatric Gastroenterology and Hepatology, Seattle Children's Hospital, Seattle, WA 98145, USA.
  • Robbings BM; University of Washington Medicine Diabetes Institute, University of Washington, Seattle, WA 98109, USA; Department of Biochemistry, University of Washington, Seattle, WA 98109, USA.
  • Hass D; Department of Biochemistry, University of Washington, Seattle, WA 98109, USA.
  • Hurley JB; Department of Biochemistry, University of Washington, Seattle, WA 98109, USA.
  • Sweet IR; University of Washington Medicine Diabetes Institute, University of Washington, Seattle, WA 98109, USA; EnTox Sciences, Inc., Mercer Island, WA 98040, USA. Electronic address: isweet@uw.edu.
Cell Rep Methods ; 3(11): 100642, 2023 Nov 20.
Article en En | MEDLINE | ID: mdl-37963464
ABSTRACT
To address the needs of the life sciences community and the pharmaceutical industry in pre-clinical drug development to both maintain and continuously assess tissue metabolism and function with simple and rapid systems, we improved on the initial BaroFuse to develop it into a fully functional, pumpless, scalable multi-channel fluidics instrument that continuously measures changes in oxygen consumption and other endpoints in response to test compounds. We and several other laboratories assessed it with a wide range of tissue types including retina, pancreatic islets, liver, and hypothalamus with both aqueous and gaseous test compounds. The setup time was less than an hour for all collaborating groups, and there was close agreement between data obtained from the different laboratories. This easy-to-use system reliably generates real-time metabolic and functional data from tissue and cells in response to test compounds that will address a critical need in basic and applied research.
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Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Islotes Pancreáticos Idioma: En Año: 2023 Tipo del documento: Article
Texto completo
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Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Islotes Pancreáticos Idioma: En Año: 2023 Tipo del documento: Article