HPLC analysis of the dissociation and recombination of rabbit immunoglobulin G.
J Immunol Methods
; 96(2): 195-9, 1987 Feb 11.
Article
en En
| MEDLINE
| ID: mdl-3805740
ABSTRACT
Rabbit immunoglobulin G (RIgG) was reduced with dithioerythritol and analyzed by high performance liquid chromatography. A quantitative method for determining the percentage of reduced half-molecules in the mixture was developed. An acetic acid concentration-dependent rate of dissociation of reduced half-molecules was observed. The specific optical absorptivity was determined for whole molecules and half-molecules and found to be significantly greater for the half-molecules. Purified half-molecules were reconstituted into RIgG with a yield greater than 90% following a 16 h incubation at pH 8.0 and room temperature.
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Banco de datos:
MEDLINE
Asunto principal:
Inmunoglobulina G
/
Cromatografía Líquida de Alta Presión
Límite:
Animals
Idioma:
En
Año:
1987
Tipo del documento:
Article