Asymmetrical nucleosomal DNA signatures regulate transcriptional directionality.
Cell Rep
; 43(1): 113605, 2024 01 23.
Article
en En
| MEDLINE
| ID: mdl-38127622
ABSTRACT
Despite the symmetrical structure of nucleosomes, in vitro studies have shown that transcription proceeds with different efficiency depending on the orientation of the DNA sequence around them. However, it is unclear whether this functional asymmetry is present in vivo and whether it could regulate transcriptional directionality. Here, we report that the proximal and distal halves of nucleosomal DNA contribute differentially to nucleosome stability in the genome. In +1 nucleosomes, this asymmetry facilitates or hinders transcription depending on the orientation of its underlying DNA, and this difference is associated with an asymmetrical interaction between DNA and histones. These properties are encoded in the DNA signature of +1 nucleosomes, since its incorporation in the two orientations into downstream nucleosomes renders them asymmetrically accessible to MNase and inverts the balance between sense and antisense transcription. Altogether, our results show that nucleosomal DNA endows nucleosomes with asymmetrical properties that modulate the directionality of transcription.
Palabras clave
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Histonas
/
Nucleosomas
Idioma:
En
Año:
2024
Tipo del documento:
Article