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Cytoplasmic accumulation and plasma membrane association of anillin and Ect2 promote confined migration and invasion.
Tran, Avery T; Wisniewski, Emily O; Mistriotis, Panagiotis; Stoletov, Konstantin; Parlani, Maria; Amitrano, Alice; Ifemembi, Brent; Lee, Se Jong; Bera, Kaustav; Zhang, Yuqi; Tuntithavornwat, Soontorn; Afthinos, Alexandros; Kiepas, Alexander; Jamieson, John J; Zuo, Yi; Habib, Daniel; Wu, Pei-Hsun; Martin, Stuart S; Gerecht, Sharon; Gu, Luo; Lewis, John D; Kalab, Petr; Friedl, Peter; Konstantopoulos, Konstantinos.
Afiliación
  • Tran AT; Department of Chemical and Biomolecular Engineering, The Johns Hopkins University, Baltimore MD, 21218, USA.
  • Wisniewski EO; Johns Hopkins Institute for NanoBioTechnology, The Johns Hopkins University, Baltimore MD, 21218, USA.
  • Mistriotis P; Department of Chemical and Biomolecular Engineering, The Johns Hopkins University, Baltimore MD, 21218, USA.
  • Stoletov K; Johns Hopkins Institute for NanoBioTechnology, The Johns Hopkins University, Baltimore MD, 21218, USA.
  • Parlani M; Department of Chemical and Biomolecular Engineering, The Johns Hopkins University, Baltimore MD, 21218, USA.
  • Amitrano A; Johns Hopkins Institute for NanoBioTechnology, The Johns Hopkins University, Baltimore MD, 21218, USA.
  • Ifemembi B; Department of Chemical Engineering, Auburn University, Auburn, AL, 36849, USA.
  • Lee SJ; Department of Oncology, University of Alberta, Edmonton, AB T6G 2E1, Canada.
  • Bera K; Department of Medical Biosciences, Radboud University Medical Center, Nijmegen, Netherlands.
  • Zhang Y; Department of Chemical and Biomolecular Engineering, The Johns Hopkins University, Baltimore MD, 21218, USA.
  • Tuntithavornwat S; Johns Hopkins Institute for NanoBioTechnology, The Johns Hopkins University, Baltimore MD, 21218, USA.
  • Afthinos A; Department of Chemical and Biomolecular Engineering, The Johns Hopkins University, Baltimore MD, 21218, USA.
  • Kiepas A; Johns Hopkins Institute for NanoBioTechnology, The Johns Hopkins University, Baltimore MD, 21218, USA.
  • Jamieson JJ; Department of Chemical and Biomolecular Engineering, The Johns Hopkins University, Baltimore MD, 21218, USA.
  • Zuo Y; Johns Hopkins Institute for NanoBioTechnology, The Johns Hopkins University, Baltimore MD, 21218, USA.
  • Habib D; Department of Chemical and Biomolecular Engineering, The Johns Hopkins University, Baltimore MD, 21218, USA.
  • Wu PH; Johns Hopkins Institute for NanoBioTechnology, The Johns Hopkins University, Baltimore MD, 21218, USA.
  • Martin SS; Department of Chemical and Biomolecular Engineering, The Johns Hopkins University, Baltimore MD, 21218, USA.
  • Gerecht S; Johns Hopkins Institute for NanoBioTechnology, The Johns Hopkins University, Baltimore MD, 21218, USA.
  • Gu L; Department of Chemical and Biomolecular Engineering, The Johns Hopkins University, Baltimore MD, 21218, USA.
  • Lewis JD; Johns Hopkins Institute for NanoBioTechnology, The Johns Hopkins University, Baltimore MD, 21218, USA.
  • Kalab P; Department of Chemical and Biomolecular Engineering, The Johns Hopkins University, Baltimore MD, 21218, USA.
  • Friedl P; Johns Hopkins Institute for NanoBioTechnology, The Johns Hopkins University, Baltimore MD, 21218, USA.
  • Konstantopoulos K; Department of Chemical and Biomolecular Engineering, The Johns Hopkins University, Baltimore MD, 21218, USA.
Res Sq ; 2024 Jan 10.
Article en En | MEDLINE | ID: mdl-38260442
ABSTRACT
Cells migrating in confinement experience mechanical challenges whose consequences on cell migration machinery remain only partially understood. Here, we demonstrate that a pool of the cytokinesis regulatory protein anillin is retained during interphase in the cytoplasm of different cell types. Confinement induces recruitment of cytoplasmic anillin to plasma membrane at the poles of migrating cells, which is further enhanced upon nuclear envelope (NE) rupture(s). Rupture events also enable the cytoplasmic egress of predominantly nuclear RhoGEF Ect2. Anillin and Ect2 redistributions scale with microenvironmental stiffness and confinement, and are observed in confined cells in vitro and in invading tumor cells in vivo. Anillin, which binds actomyosin at the cell poles, and Ect2, which activates RhoA, cooperate additively to promote myosin II contractility, and promote efficient invasion and extravasation. Overall, our work provides a mechanistic understanding of how cytokinesis regulators mediate RhoA/ROCK/myosin II-dependent mechanoadaptation during confined migration and invasive cancer progression.
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Texto completo: 1 Banco de datos: MEDLINE Tipo de estudio: Risk_factors_studies Idioma: En Año: 2024 Tipo del documento: Article
Texto completo
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Banco de datos: MEDLINE Tipo de estudio: Risk_factors_studies Idioma: En Año: 2024 Tipo del documento: Article