A novel frameshift mutation in RHAG leads to Rhnull phenotype in a Chinese individual.
Transfusion
; 64(5): 789-792, 2024 May.
Article
en En
| MEDLINE
| ID: mdl-38562113
ABSTRACT
BACKGROUND:
We recently encountered a Rhnull phenotype proband within one family in the Chinese population. Rhnull is a rare autosomal recessive disorder characterized by the absence of the Rh antigens on the erythrocyte membrane, resulting in chronic hemolytic anemia. This study described the serological and molecular analysis of a Chinese Rhnull proband and his immediate family.METHODS:
Red blood cells antigen phenotyping and antibody screening/identification were conducted. RHD, RHCE, and RHAG were analyzed using genomic DNA by polymerase chain reaction and sequence analysis.RESULTS:
Serologic tests showed a D-C-E-c-e- phenotype in the proband associated with the suspicion of anti-Rh29 (titer 16). Molecular analyses showed a new mutation (c.406dupA) in exon 3 of RHAG. This duplication introduced a reading frameshift (p.Thr136AsnfsTer21). The RHAG mutation was found in the homozygous state for the proband and heterozygous state for his parents.CONCLUSION:
We identified a novel RHAG mutation resulting in the Rhnull phenotype of the regulator type. Inheritance of the novel allele was shown by family study.Palabras clave
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Fenotipo
/
Sistema del Grupo Sanguíneo Rh-Hr
/
Mutación del Sistema de Lectura
Límite:
Female
/
Humans
/
Male
Idioma:
En
Año:
2024
Tipo del documento:
Article