A chemiluminescence immunoassay for type IV collagen as a promising marker for liver fibrosis and cirrhosis.
Anal Methods
; 16(15): 2248-2255, 2024 04 18.
Article
en En
| MEDLINE
| ID: mdl-38568684
ABSTRACT
Herein, a magnetic bead-based chemiluminescence assay is reported to detect type IV collagen (col-IV) in serum samples. Magnetic beads (MBs) exhibit biocompatibility. Taking advantage of this property, they were conjugated with the col-IV antibody. For the determination of col-IV, the interaction of the col-IV sample, anti-(col-IV)-alkaline phosphatase (anti-(col-IV)-ALP) and anti-col-IV-magnetic beads (anti-(col-IV)-MBs) was performed to generate chemiluminescence. Under the optimized conditions, the developed method displayed good linearity in the concentration range of 20-2000 ng mL-1 with the limit of 0.79 ng mL-1. The repeatability coefficient of variation (CV) for col-IV detection ranged from 3.16% to 7.50%. The col-IV level in samples collected from a hospital was assessed by the chemiluminescence assay. Satisfactory recoveries were obtained ranging from 93.30% to 100.14%. In conclusion, the magnetic bead-based chemiluminescence assay may be used as a routine and efficient tool to detect type IV collagen in clinical diagnosis.
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Colágeno Tipo IV
/
Luminiscencia
Límite:
Humans
Idioma:
En
Año:
2024
Tipo del documento:
Article