Contributions of the individual domains of αIIbß3 integrin to its extension: Insights from multiscale modeling.
Cytoskeleton (Hoboken)
; 81(8): 393-408, 2024 Aug.
Article
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| MEDLINE
| ID: mdl-38682753
ABSTRACT
The platelet integrin αIIbß3 undergoes long-range conformational transitions between bent and extended conformations to regulate platelet aggregation during hemostasis and thrombosis. However, how exactly αIIbß3 transitions between conformations remains largely elusive. Here, we studied how transitions across bent and extended-closed conformations of αIIbß3 integrin are regulated by effective interactions between its functional domains. We first carried out µs-long equilibrium molecular dynamics (MD) simulations of full-length αIIbß3 integrins in bent and intermediate conformations, the latter characterized by an extended headpiece and closed legs. Then, we built heterogeneous elastic network models, perturbed inter-domain interactions, and evaluated their relative contributions to the energy barriers between conformations. Results showed that integrin extension emerges from (i) changes in interfaces between functional domains; (ii) allosteric coupling of the head and upper leg domains with flexible lower leg domains. Collectively, these results provide new insights into integrin conformational activation based on short- and long-range interactions between its functional domains and highlight the importance of the lower legs in the regulation of integrin allostery.
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Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Complejo GPIIb-IIIa de Glicoproteína Plaquetaria
/
Dominios Proteicos
Límite:
Humans
Idioma:
En
Año:
2024
Tipo del documento:
Article