Your browser doesn't support javascript.
loading
Characterization of a lipid-based jumbo phage compartment as a hub for early phage infection.
Mozumdar, Deepto; Fossati, Andrea; Stevenson, Erica; Guan, Jingwen; Nieweglowska, Eliza; Rao, Sanjana; Agard, David; Swaney, Danielle L; Bondy-Denomy, Joseph.
Afiliación
  • Mozumdar D; Department of Immunology and Microbiology, University of California, San Francisco, San Francisco, CA 94158, USA.
  • Fossati A; J. David Gladstone Institutes, San Francisco, CA 94158, USA; Quantitative Biosciences Institute (QBI), University of California, San Francisco, San Francisco, CA 94158, USA; Department of Cellular and Molecular Pharmacology, University of California, San Francisco, San Francisco, CA 94158, USA.
  • Stevenson E; J. David Gladstone Institutes, San Francisco, CA 94158, USA; Quantitative Biosciences Institute (QBI), University of California, San Francisco, San Francisco, CA 94158, USA; Department of Cellular and Molecular Pharmacology, University of California, San Francisco, San Francisco, CA 94158, USA.
  • Guan J; Department of Immunology and Microbiology, University of California, San Francisco, San Francisco, CA 94158, USA.
  • Nieweglowska E; Department of Biochemistry and Biophysics, University of California, San Francisco, San Francisco, CA 94158, USA.
  • Rao S; Department of Immunology and Microbiology, University of California, San Francisco, San Francisco, CA 94158, USA.
  • Agard D; Department of Biochemistry and Biophysics, University of California, San Francisco, San Francisco, CA 94158, USA; Chan Zuckerberg Imaging Institute, Redwood City, CA 94065, USA.
  • Swaney DL; J. David Gladstone Institutes, San Francisco, CA 94158, USA; Quantitative Biosciences Institute (QBI), University of California, San Francisco, San Francisco, CA 94158, USA; Department of Cellular and Molecular Pharmacology, University of California, San Francisco, San Francisco, CA 94158, USA.
  • Bondy-Denomy J; Department of Immunology and Microbiology, University of California, San Francisco, San Francisco, CA 94158, USA; Quantitative Biosciences Institute (QBI), University of California, San Francisco, San Francisco, CA 94158, USA. Electronic address: joseph.bondy-denomy@ucsf.edu.
Cell Host Microbe ; 32(7): 1050-1058.e7, 2024 Jul 10.
Article en En | MEDLINE | ID: mdl-38870941
ABSTRACT
Viral genomes are most vulnerable to cellular defenses at the start of the infection. A family of jumbo phages related to phage ΦKZ, which infects Pseudomonas aeruginosa, assembles a protein-based phage nucleus to protect replicating phage DNA, but how it is protected prior to phage nucleus assembly is unclear. We find that host proteins related to membrane and lipid biology interact with injected phage protein, clustering in an early phage infection (EPI) vesicle. The injected virion RNA polymerase (vRNAP) executes early gene expression until phage genome separation from the vRNAP and the EPI vesicle, moving into the nascent proteinaceous phage nucleus. Enzymes involved in DNA replication and CRISPR/restriction immune nucleases are excluded by the EPI vesicle. We propose that the EPI vesicle is rapidly constructed with injected phage proteins, phage DNA, host lipids, and host membrane proteins to enable genome protection, early transcription, localized translation, and to ensure faithful genome transfer to the proteinaceous nucleus.
Asunto(s)
Palabras clave

Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Pseudomonas aeruginosa / ADN Viral / Genoma Viral / Fagos Pseudomonas Idioma: En Año: 2024 Tipo del documento: Article

Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Pseudomonas aeruginosa / ADN Viral / Genoma Viral / Fagos Pseudomonas Idioma: En Año: 2024 Tipo del documento: Article