Exploring the presence, genomic traits, and pathogenic potential of extended-spectrum ß-lactamase Escherichia coli in freshwater, wastewater, and hospital effluents.
J Appl Microbiol
; 135(7)2024 Jul 02.
Article
en En
| MEDLINE
| ID: mdl-38906843
ABSTRACT
AIMS:
The purpose of this work was to study extended-spectrum ß-lactamase (ESBL)-producing Escherichia coli (ESBL-EC) in freshwaters, hospital effluents, and wastewaters during two sampling campaigns in 2021. METHODS ANDRESULTS:
Water sampling was performed at 24 stations in the Ourthe watershed in Belgium. A total of 644 ESBL (n = 642) and AmpC (n = 2) E. coli strains were isolated. Disk-diffusion assays were performed following the EUCAST's recommendations. All strains were tested for the presence of blaCTX-M-1, blaCTX-M-2, and blaCTX-M-9 gene groups by PCR. Genes belonging to blaCTX-M-1 and blaCTX-M-9 groups were detected, respectively, in 73.6% and 14.9% of the strains. No blaCTX-M-2 group's gene was found. A subset of strains (n = 40) was selected for whole genome sequencing. Escherichia coli serotype O18 H7 ST 1463 was predominant (n = 14) in the sequenced strains and showed pathogenicity in the Galleria mellonella larvae model. ß-lactamase genes identified were blaCTX-M (n = 21), with blaCTX-M-15 mostly represented (n = 15), as well as blaTEM (n = 11), blaOXA (n = 7), blaSHV (n = 9), and carbapenemase (CP) genes were observed in several strains-blaKPC-3 (n = 19), blaNDM-1 (n = 1), blaVIM-1 (n = 2), and blaOXA-244 (n = 2)-even from freshwaters.CONCLUSIONS:
ESBL-EC are widely distributed in the aquatic environment in Belgium and contain a variety of ESBL and CP genes.Palabras clave
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Beta-Lactamasas
/
Escherichia coli
/
Aguas Residuales
/
Agua Dulce
/
Hospitales
Límite:
Animals
País/Región como asunto:
Europa
Idioma:
En
Año:
2024
Tipo del documento:
Article