Stable Expression by Lentiviral Transduction of Cells.
Methods Mol Biol
; 2810: 147-159, 2024.
Article
en En
| MEDLINE
| ID: mdl-38926278
ABSTRACT
Lentiviral gene transfer represents a versatile and powerful method for genetic transduction of many cell lines and primary cells including "hard-to-transfect" cells. As a consequence of the integration of the recombinant lentiviral vector into the cellular genome, the transgene is stably maintained, and long-term producing cells are established. Here, we describe the current state of the art and give details for lab-scale production of lentiviral vectors as well as for infection and titration of the viral vectors.
Palabras clave
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Transducción Genética
/
Lentivirus
/
Vectores Genéticos
Límite:
Humans
Idioma:
En
Año:
2024
Tipo del documento:
Article