A simple method for the identification of compounds which inhibit tubulin polymerization in filarial worms.
Ann Trop Med Parasitol
; 79(5): 507-12, 1985 Oct.
Article
en En
| MEDLINE
| ID: mdl-4083960
ABSTRACT
The incubation in vitro of excised ovaries of Dirofilaria immits in medium containing mebendazole between 10(-5) and 10(-8) M for four or six hours results in the accumulation of up to 20% of oogonial cells in arrested mitotic metaphase. In aceto-orcein-stained squashes of the tissue, cells possess condensed chromosomes but no detectable spindle microtubules. Similar results were obtained with colchicine, but the lowest effective concentration of this drug was 10(-7) M. This procedure affords a simple and rapid method for detecting compounds capable of inhibiting tubulin polymerization in filarial worms.
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Banco de datos:
MEDLINE
Asunto principal:
Tubulina (Proteína)
/
Dirofilaria
/
Filarioidea
/
Mebendazol
Tipo de estudio:
Diagnostic_studies
Límite:
Animals
Idioma:
En
Año:
1985
Tipo del documento:
Article