Megakaryocyte colony formation from human bone marrow precursors.
Blood
; 54(4): 940-5, 1979 Oct.
Article
en En
| MEDLINE
| ID: mdl-476307
ABSTRACT
We report the growth in plasma clot culture of megakaryocyte colonies from adult bone marrow cells with the use of four different sources of erythropoietin (Ep) as the stimulating factor. A major proportion of the megakaryocyte colonies (75%) were pure, while the others were mixed, involving erythroblasts and megakaryocytes. Ultrastructural studies have shown that the maturation of megakaryocytes was essentially normal and that platelet shedding occurred. Megakaryocyte colony formation required a large number of plated cells (greater than 3 X 10(5)/ml). In the absence of erythropoietin, rare spontaneous megakaryocyte colonies could be observed, while no erythroid colonies were present. However, erythropoietin induced a fivefold increase in the total number of colonies. These data suggest that erythropoietin is involved in the differentiation of human megakaryocytes, but that it does not act alone, since another factor related to the number of seeded cells appears essential for the formation of human megakaryocyte colonies.
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Banco de datos:
MEDLINE
Asunto principal:
Megacariocitos
/
Ensayo de Unidades Formadoras de Colonias
Límite:
Animals
/
Humans
Idioma:
En
Año:
1979
Tipo del documento:
Article