Ecto-adenosine triphosphatase deficiency in cultured human T and null leukemic lymphocytes. A biochemical basis for thymidine sensitivity.
J Clin Invest
; 68(2): 544-52, 1981 Aug.
Article
en En
| MEDLINE
| ID: mdl-6114965
ABSTRACT
Cultured leukemic T and null lymphocytes are highly sensitive to growth inhibition by thymidine, as well as the other deoxynucleosides, deoxyguanosine and deoxyadenosine. By contrast, Epstein-Barr virus-transformed B lymphocytes are relatively resistant to deoxynucleosides. Growth inhibition is associated with the development of high deoxyribotriphosphate pools after exposure to the respective deoxynucleotides. We show that malignant T and null lymphocytes are deficient in ecto-ATPase activity. We show this cell surface enzyme to be of broad specificity, capable of degrading both ribotriphosphates and deoxyribotriphosphates. High levels of this ecto-enzyme are found in deoxynucleoside-resistant, Epstein-Barr virus-transformed B lymphocytes. Ecto-ATPase deficiency may represent a mechanism for increased sensitivity to deoxynucleoside growth inhibition.
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Timidina
/
Linfocitos
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Linfocitos T
/
Leucemia
/
Adenosina Trifosfatasas
Tipo de estudio:
Diagnostic_studies
Límite:
Humans
Idioma:
En
Año:
1981
Tipo del documento:
Article