Phenotype study with monoclonal antibodies of T lymphocyte colonies in normal individuals and in patients with chronic OKT8+ lymphocytic leukaemia.
Clin Exp Immunol
; 54(2): 515-24, 1983 Nov.
Article
en En
| MEDLINE
| ID: mdl-6606509
ABSTRACT
The lymphocyte colony forming capacity of peripheral blood mononuclear cells from normal controls and from two patients with chronic OKT8+ lymphocytic leukaemia was determined in agar culture under PHA stimulation. The number and size of the colonies in patients were reduced compared to normal. The lymphocytic phenotype of colony cells was studied with monoclonal antibodies in colonies harvested from agar culture and in colonies expanded in liquid culture in the presence of TCGF. This study was performed in individual colonies and in pooled colonies. Colonies from normal controls contained a mixture of the OKT4+ and OKT8+ lymphocyte subsets. In contrast, colonies from the two patients contained essentially OKT4+ lymphocytes. The data indicate that, in the patients, progenitors of the OKT8+ subset are unresponsive to normal proliferative and/or differentiative stimuli under the present culture conditions.
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Linfocitos T
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Leucemia Linfoide
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Anticuerpos Monoclonales
Límite:
Aged
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Female
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Humans
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Male
Idioma:
En
Año:
1983
Tipo del documento:
Article