Heterogeneous accessory cell requirement for human peripheral blood T lymphocyte activation by PHA into IL-2-responsive colony-forming cells.
Cell Immunol
; 87(1): 167-76, 1984 Aug.
Article
en En
| MEDLINE
| ID: mdl-6611211
ABSTRACT
Mitogen-driven T cell proliferation in liquid culture requires accessory cells that cooperate in interleukin 2 production. We have investigated the accessory cell requirement for human lymphocyte colony formation under PHA stimulation. Semisolid medium limits cell-to-cell contact emphasizing the role of cooperating cells both in growth factor production and in triggering events. Culturing at high T cell density demonstrates that accessory cells can be substituted for colony formation by exogenous IL-2. Culturing at low T cell density in the presence of IL-2 also demonstrates that accessory cells are required for activation of a subset of progenitors into IL-2 responsive colony-forming cells. Consequently, T colony progenitors, contained in the E-rosetting cell fraction of peripheral blood, are heterogeneous in their triggering signals a minor subset is directly inducible by PHA, and a major subset is inducible by PHA in the presence of accessory cells. We found that monocytes and some leukemic B cells support effective accessory function in both colony growth factor production and colony progenitor sensitization.
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Banco de datos:
MEDLINE
Asunto principal:
Células Madre Hematopoyéticas
/
Activación de Linfocitos
/
Linfocitos T
/
Interleucina-2
/
Cooperación Linfocítica
Límite:
Humans
Idioma:
En
Año:
1984
Tipo del documento:
Article