Two FIA-based biosensor systems studied for bioprocess monitoring.
J Biotechnol
; 31(3): 345-56, 1993 Dec.
Article
en En
| MEDLINE
| ID: mdl-7764442
ABSTRACT
In this paper, two different FIA-based biosensor systems are described for application to different biotechnologically relevant purposes. In the first system, single fiber optodes were used to determine the pH, urea and penicillin V concentrations. A two-channel system was developed for the simultaneous monitoring of different variables to increase the analysis accuracy. This system was used for monitoring the penicillin V concentration during a cultivation of Penicillium chrysogenum. The second system described is a calorimetric immunoassay based on the use of an enzyme thermistor. A sandwich assay with protein A immobilized on a solid support for the determination of various IgGs was established. A fusion protein of protein A and beta-galactosidase obtained from a recombinant E. coli strain was used in the labelling and detection reaction. This system is designed for future application in bioprocess monitoring.
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Banco de datos:
MEDLINE
Asunto principal:
Penicillium
/
Urea
/
Inmunoglobulina G
/
Técnicas Biosensibles
/
Diálisis Renal
/
Penicilina V
/
Concentración de Iones de Hidrógeno
Límite:
Humans
Idioma:
En
Año:
1993
Tipo del documento:
Article