Prenatal diagnosis of sickle cell anemia using PCR and restriction enzyme Dde I.
Turk J Pediatr
; 35(3): 159-62, 1993.
Article
en En
| MEDLINE
| ID: mdl-7909397
ABSTRACT
Prenatal diagnosis of sickle cell anemia was carried out in four fetuses using DNA technology. Fetal chorionic villus specimen were obtained at the 10th week of pregnancy from women at risk of giving birth to children with sickle cell anemia. Whole cellular DNA was obtained and the part of the DNA presumed to have a mutation increased after PCR was performed. After the application of Dde I restriction enzyme, mini gel electrophoresis was performed. The study of the electrophoretic patterns of the DNA indicated that one of the four fetuses was unaffected, one was a carrier and the remaining two were affected.
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Banco de datos:
MEDLINE
Asunto principal:
Diagnóstico Prenatal
/
Desoxirribonucleasas de Localización Especificada Tipo I
/
Reacción en Cadena de la Polimerasa
/
Enfermedades Fetales
/
Anemia de Células Falciformes
Tipo de estudio:
Diagnostic_studies
Límite:
Female
/
Humans
/
Pregnancy
Idioma:
En
Año:
1993
Tipo del documento:
Article