Polymerase chain reaction detection of Plasmodium falciparum in mosquitoes.
Trans R Soc Trop Med Hyg
; 87(3): 273-5, 1993.
Article
en En
| MEDLINE
| ID: mdl-8236389
ABSTRACT
The polymerase chain reaction (PCR) procedure using a primer set derived from a repetitive deoxyribonucleic acid (DNA) sequence specific to Plasmodium falciparum was used to detect parasite DNA in mosquitoes. In laboratory-infected mosquitoes, PCR could detect as few as 10 sporozoites in a dissected salivary gland and a single oocyst in a dissected midgut. The ability to detect P. falciparum DNA in wild-caught mosquitoes indicated an advantage of the PCR over enzyme-linked immunosorbent assay for the detection of Plasmodium sporozoites in mosquitoes with low-grade parasite infections.
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Banco de datos:
MEDLINE
Asunto principal:
Plasmodium falciparum
/
Anopheles
Tipo de estudio:
Diagnostic_studies
Límite:
Animals
Idioma:
En
Año:
1993
Tipo del documento:
Article