Interaction of GTP-binding protein Gq with photoactivated rhodopsin in the photoreceptor membranes of crayfish.
FEBS Lett
; 330(2): 197-200, 1993 Sep 13.
Article
en En
| MEDLINE
| ID: mdl-8365491
ABSTRACT
Interaction of G-protein with photoactivated rhodopsin (Rh*) in crayfish photoreceptor membranes was investigated by immunoprecipitation using an antibody against rhodopsin. Two kinds of protein were co-precipitated with rhodopsin. One is an alpha subunit of class-q G-protein (42 kDa, CGq alpha) which showed light-induced, dose-dependent binding to rhodopsin, and the other is an actin-like protein (44 kDa) with light-independent binding. Most of the CGq alpha was available for binding to Rh* but was dissociated from Rh* in the presence of GTP gamma S. These findings demonstrate that, in the crayfish photoreceptor, a Gq class of G-protein is activated by Rh*.
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Banco de datos:
MEDLINE
Asunto principal:
Células Fotorreceptoras
/
Rodopsina
/
Proteínas de Unión al GTP
Límite:
Animals
Idioma:
En
Año:
1993
Tipo del documento:
Article