Antioxidant effects of hyaluronan and its alpha-methyl-prednisolone derivative in chondrocyte and cartilage cultures.
Semin Arthritis Rheum
; 26(1): 492-501, 1996 Aug.
Article
en En
| MEDLINE
| ID: mdl-8870117
ABSTRACT
OBJECTIVE:
To compare hyaluronan and its alpha-methyl prednisolone derivative (HYC-141) with respect to their potential to directly cause tissue damage and to protect tissues from attack by reactive oxygen species.METHODS:
Cartilage samples and chondrocytes were isolated from 15-day chick embryos and were exposed to enzyme-based and activated inflammatory cell-free radical generating systems in the presence of varying concentrations of alpha-methyl prednisolone, hyaluronan, and HYC-141. Tissue and cell vitality was measured by determining the incorporation of radioactive sulphate into newly synthesized glycosaminoglycans.RESULTS:
Only alpha-methyl prednisolone caused a significant decrease in biosynthetic activity. All the tested substances were capable, to some extent, of protecting tissues and cells from damage by reactive oxygen species; HYC-141 demonstrated the greatest protective effect.CONCLUSION:
These data suggest that HYC-141 may possess certain advantages over the individual component molecules in the local treatment of arthropathies.
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Banco de datos:
MEDLINE
Asunto principal:
Prednisolona
/
Cartílago Articular
/
Ácido Hialurónico
/
Antiinflamatorios
/
Antioxidantes
Límite:
Animals
Idioma:
En
Año:
1996
Tipo del documento:
Article