ABSTRACT
Two new gallic acid glycosides, potentillanosides G (1) and H (2), were newly isolated from the methanol extract of the tuberous roots of Potentilla anserina (Rosaceae), together with a known compound, ellagic acid 3-O-α-L-rhamnopyranoside (3). Their structures were elucidated on the basis of chemical and physicochemical evidence. Among the constituents, potentillanoside H (2, IC50 = 99.5 µM) was found to show hepatoprotective activity.
Subject(s)
Ellagic Acid/isolation & purification , Glycosides/isolation & purification , Hepatocytes/drug effects , Plant Extracts/isolation & purification , Potentilla/chemistry , Acetates/chemistry , Animals , Cell Survival/drug effects , Cells, Cultured , Cytoprotection , Ellagic Acid/pharmacology , Glycosides/pharmacology , Hepatocytes/physiology , Inhibitory Concentration 50 , Medicine, Tibetan Traditional , Methanol/chemistry , Mice , Molecular Structure , Plant Extracts/pharmacology , Plant Roots/chemistry , Solvents/chemistryABSTRACT
A methanol extract from the tuberous roots of Potentilla anserina (Rosaceae) exhibited hepatoprotective effects against d-galactosamine (d-GalN)/lipopolysaccharide-induced liver injuries in mice. Six triterpene 28-O-monoglucopyranosyl esters, potentillanosides A-F, were isolated from the extract along with 32 known compounds, including 15 triterpenes. The structures of potentillanosides A-F were determined on the basis of spectroscopic properties and chemical evidence. Four ursane-type triterpene 28-O-monoglycosyl esters, potentillanoside A (IC50=46.7µM), 28-O-ß-d-glucopyranosyl pomolic acid (IC50=9.5µM), rosamutin (IC50=35.5µM), and kaji-ichigoside F1 (IC50=14.1µM), inhibited d-GalN-induced cytotoxicity in primary cultured mouse hepatocytes. Among these four triterpenes, potentillanoside A, rosamutin, and kaji-ichigoside F1 exhibited in vivo hepatoprotective effects at doses of 50-100mg/kg, p.o. The mode of action was ascribable to the reduction in cytotoxicity caused by d-GalN.