Does histological prostatic inflammation during transurethral resection of the prostate for bladder outlet obstruction affect post-operative urinary outcomes?

OBJECTIVE: Benign prostate hyperplasia (BPH) is a common cause for bladder outlet obstruction (BOO) and lower urinary tract symptoms (LUTS) in men. The pathophysiology of BPH is multifactorial and inflammation has been linked with progression of BPH and LUTS. The association between histological prostatitis found at transurethral resection of the prostate (TURP) and adverse post-operative urinary outcomes is not clearly defined. Our aim was to evaluate the association between histological prostatitis and adverse post-operative urinary outcomes following TURP procedure. METHODS: Patients who had undergone TURP for BPH at a single institution between 2014 and 2018 were included. The study population was divided into three cohorts: those with no histological inflammation, those with any form of inflammation and those specifically with prostatic stromal inflammation. Functional outcomes were assessed by defining a series of measurable post-operative "LUTS events" and comparing these to time-to-event profile using a Kaplan-Meier estimator. RESULTS: A total 198 patients were included (no inflammation n = 101; any inflammation n = 97, prostatic stromal inflammation n = 81). All three groups were comparable in terms of baseline characteristics. The any inflammation group had significantly more adverse post-operative outcomes after TURP compared to the no inflammation group, P = 0.0065. The stromal inflammation group had more LUTS events after surgery compared to the no inflammation groups in the first year of follow-up n = 0.011; over a 5-year follow-up period the results were not statistically significant, P = 0.244. CONCLUSION: Histological prostatitis is associated with worse urinary outcomes after TURP compared to no inflammation. These results are useful in improving prognostic discussions with patients after TURP.

Identification of a vicilin-like major allergen from Prosopis juliflora exhibiting cross- reactivity with legume food allergens.

BACKGROUND: Prosopis juliflora is a clinically relevant allergic sensitizer worldwide and shares cross-reactivity with allergens from several tree pollen and food. The present study aims to purify and immunobiochemically characterize a major allergen from Prosopis pollen. The allergen was further investigated for its cross-reactivity with legume allergens. METHODS: Prosopis extract was fractionated by Q Sepharose and Superdex 75 gel filtration column to purify the allergen. Specific IgE against purified protein was estimated via ELISA and immunoblot. The protein was subjected to mass spectrometric analysis. Glycan characterization was performed by Schiff staining and lectin binding assay followed by deglycosylation studies. The functional activity of the purified protein was evaluated by the basophil activation test. Cross-reactivity was assessed by inhibition studies with legume extracts. RESULTS: A 35 kDa protein was purified and showed 75% IgE reactivity with the patients' sera by ELISA and immunoblot. Glycan characterization of protein demonstrated the presence of terminal glucose and mannose residues. A reduction of 40% and 27% in IgE binding was observed upon chemical and enzymatic deglycosylation of the protein, respectively. The glycoprotein allergen upregulates the expression of CD203c on basophils which was significantly reduced upon deglycosylation, signifying its biological ability to activate the effector cells. The identified protein shared significant homology with Lup an 1 from the lupine bean. Immunoblot inhibition studies of the purified allergen with legume extracts underlined high cross-reactive potential. Complete inhibition was observed with peanut and common bean, while up to 70% inhibition was demonstrated with soy, black gram, chickpea, and lima bean. CONCLUSION: A 35 kDa vicilin-like major allergen was isolated from P. juliflora. The protein possesses glycan moieties crucial for IgE binding and basophil activation. Furthermore, the purified protein shows homology with Lup an 1 and exhibits cross-reactivity with common edible legume proteins.

Uso de nosódios na prática clínica: resultados de uma enquete / Use of nosodes in homeopathic clinical practice: a survey

Os nosódios são preparações homeopáticas de materiais orgânicos derivados de produtos inativos de doenças, culturas de microrganismos (bactérias, vírus e fungos, por exemplo) ou parasitas, material infectado ou com alterações patológicas ou produtos de decomposição animal ou humana feitos seguros através do procedimento farmacotécnico homeopático. Os nosódios têm papel importante na prática clínica. São frequentemente indicados como medicamentos usuais, intercorrentes, antimiasmáticos, agudos, etc., de acordo com a percepção individual de cada profissional. Assim, há variações no uso clínico desses medicamentos, que pode estar influenciado pelo conhecimento, experiência, proficiência e noções preconcebidas dos profissionais. A presente pesquisa objetivou investigar o uso real dos nosódios na prática clínica homeopática. (AU)

Nosodes are homeopathic preparations of organic materials derived from inactivated disease products, cultures of microorganisms (e.g., bacteria, fungi and viruses) or parasites, infected or pathologically changed material or decomposition products from humans or animals, rendered safe during the homeopathic manufacturing process. In homeopathic clinical practice, nosodes have an important and indispensable part. They are frequently indicated as common, intercurrent, anti-miasmatic, or acute remedies, etc., depending on the physician’s perception. But there are variations in their clinical use, which might be influenced by the background knowledge, experience, expertise and preconceived notions of practitioners. The present survey sought to find the actual use made of nosodes in homeopathic clinical practice. (AU)

Uso de nosódios na prática clínica: resultados de uma enquete / Use of nosodes in homeopathic clinical practice: a survey

Os nosódios são preparações homeopáticas de materiais orgânicos derivados de produtos inativos de doenças, culturas de microrganismos (bactérias, vírus e fungos, por exemplo) ou parasitas, material infectado ou com alterações patológicas ou produtos de decomposição animal ou humana feitos seguros através do procedimento farmacotécnico homeopático. Os nosódios têm papel importante na prática clínica. São frequentemente indicados como medicamentos usuais, intercorrentes, antimiasmáticos, agudos, etc., de acordo com a percepção individual de cada profissional. Assim, há variações no uso clínico desses medicamentos, que pode estar influenciado pelo conhecimento, experiência, proficiência e noções preconcebidas dos profissionais. A presente pesquisa objetivou investigar o uso real dos nosódios na prática clínica homeopática.

Nosodes are homeopathic preparations of organic materials derived from inactivated disease products, cultures of microorganisms (e.g., bacteria, fungi and viruses) or parasites, infected or pathologically changed material or decomposition products from humans or animals, rendered safe during the homeopathic manufacturing process. In homeopathic clinical practice, nosodes have an important and indispensable part. They are frequently indicated as common, intercurrent, anti-miasmatic, or acute remedies, etc., depending on the physician’s perception. But there are variations in their clinical use, which might be influenced by the background knowledge, experience, expertise and preconceived notions of practitioners. The present survey sought to find the actual use made of nosodes in homeopathic clinical practice.