ABSTRACT
BACKGROUND: Epidemiological and experimental carcinogenesis studies provide evidence that components of garlic (Allium sativum) have anticancer activity. Hepatocellular carcinoma is highly malignant and metastatic. Currently, there is no effective chemotherapy for patients with advanced Hepatocellular carcinoma leading to an urgent need to seek for novel therapeutic options. AIM: To investigate the effect of cell growth, cell apoptosis of Garlic-Derived Compound S-Allylmercaptocysteine (SAMC) on Human Gastric Cancer Cells Line SGC 7901 cells. MATERIALS AND METHODS: The SGC 7901 cells were cultured with different concentration's SAMC. Cell viability was detected by AO/EB staining. JNK and P38 pathway were assayed by PCR (polymerase chain reaction). RESULTS: The best concentration of SAMC (300 µM) for induction SGC 7901 apoptosis was confirmed through cell viability. The PCR assay demonstrated that JNK and P38 pathway play important role in apoptosis of SGC 7901 cells. CONCLUSIONS: This study indicated that SAMC can inhibit cell proliferation and induct apoptosis of SGC 7901 cells via JNK and P38 pathway.
Subject(s)
Antineoplastic Agents, Phytogenic/pharmacology , Apoptosis/drug effects , Cysteine/analogs & derivatives , Stomach Neoplasms/drug therapy , Cell Line, Tumor , Cysteine/pharmacology , Garlic/chemistry , Humans , MAP Kinase Signaling System/drug effects , Stomach Neoplasms/enzymology , Stomach Neoplasms/pathologyABSTRACT
OBJECTIVE: To explore the therapeutical mechanism of Taozhi Zhipo Granule (TZZPG) on intestinal adhesion. METHODS: Fed SD male rats of intestinal adhesion model with TZZPG 10, 20 g/kg.d continuously for 12 days. RESULTS: TZZPG could alleviate the degree and the average length of intestinal adhesion in rats. It also could prolong bleeding time in mice and plasma recuperation time of guinea-pigs, improve the auricular microcirculation in mice, accelerate the removing speed of charcoal powder in mice's intestine and slightly strengthen the contraction of rat intestine in vitro. TZZPG relieved the croton oil topical edema of ear and reduced the capillary permeability of abdominal cavity in mice. TZZPG could enhance the phagocytosis of peritoneal macrophages. CONCLUSIONS: TZZPG has preventive effect on intestinal adhesion. The action might be due to improve the ischemia in local tissue, strengthen the contraction of intestine and inhibit the inflammation.