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1.
Microb Pathog ; 190: 106613, 2024 May.
Article in English | MEDLINE | ID: mdl-38484919

ABSTRACT

This research paper presents a novel approach to the green synthesis of silver nanoparticles (AgNPs) using viticultural waste, allowing to obtain NP dispersions with distinct properties and morphologies (monodisperse and polydisperse AgNPs, referred to as mAgNPs and pAgNPs) and to compare their biological activities. Our synthesis method utilized the ethanolic extract of Vitis vinifera pruning residues, resulting in the production of mAgNPs and pAgNPs with average sizes of 12 ± 5 nm and 19 ± 14 nm, respectively. Both these AgNPs preparations demonstrated an exceptional stability in terms of size distribution, which was maintained for one year. Antimicrobial testing revealed that both types of AgNPs inhibited either the growth of planktonic cells or the metabolic activity of biofilm sessile cells in Gram-negative bacteria and yeasts. No comparable activity was found towards Gram-positives. Overall, pAgNPs exhibited a higher antimicrobial efficacy compared to their monodisperse counterparts, suggesting that their size and shape may provide a broader spectrum of interactions with target cells. Both AgNP preparations showed no cytotoxicity towards a human keratinocyte cell line. Furthermore, in vivo tests using a silkworm animal model indicated the biocompatibility of the phytosynthesized AgNPs, as they had no adverse effects on insect larvae viability. These findings emphasize the potential of targeted AgNPs synthesized from viticultural waste as environmentally friendly antimicrobial agents with minimal impact on higher organisms.


Subject(s)
Metal Nanoparticles , Microbial Sensitivity Tests , Silver , Vitis , Silver/pharmacology , Silver/chemistry , Silver/metabolism , Metal Nanoparticles/chemistry , Animals , Humans , Vitis/chemistry , Plant Extracts/pharmacology , Plant Extracts/chemistry , Anti-Infective Agents/pharmacology , Anti-Infective Agents/chemistry , Particle Size , Green Chemistry Technology , Gram-Negative Bacteria/drug effects , Bombyx , Biofilms/drug effects , Cell Line , Cell Survival/drug effects , Keratinocytes/drug effects , Larva/drug effects , Yeasts/drug effects
2.
PLoS One ; 17(8): e0272844, 2022.
Article in English | MEDLINE | ID: mdl-35947573

ABSTRACT

Green methods have become vital for sustainable development of the scientific and commercial sphere; however, they can bring new challenges, including the need for detailed characterization and elucidation of efficacy of their products. In this study, green method of silver nanoparticles (AgNPs) production was employed using an extract from grapevine canes. The aim of the study was to contribute to the knowledge about biosynthesized AgNPs by focusing on elucidation of their antifungal efficiency based on their size and/or hypothesized synergy with bioactive substances from Vitis vinifera cane extract. The antifungal activity of AgNPs capped and stabilized with bioactive compounds was tested against the opportunistic pathogenic yeast Candida albicans. Two dispersions of nanoparticles with different morphology (characterized by SEM-in-STEM, DLS, UV-Vis, XRD, and AAS) were prepared by modification of reaction conditions suitable for economical production and their long-term stability monitored for six months was confirmed. The aims of the study included the comparison of the antifungal effect against suspension cells and biofilm of small monodisperse AgNPs with narrow size distribution and large polydisperse AgNPs. The hypothesis of synergistic interaction of biologically active molecules from V. vinifera extracts and AgNPs against both cell forms were tested. The interactions of all AgNPs dispersions with the cell surface and changes in cell morphology were imaged using SEM. All variants of AgNPs dispersions were found to be active against suspension and biofilm cells of C. albicans; nevertheless, surprisingly, larger polydisperse AgNPs were found to be more effective. Synergistic action of nanoparticles with biologically active extract compounds was proven for biofilm cells (MBIC80 20 mg/L of polydisperse AgNPs in extract), while isolated nanoparticles suspended in water were more active against suspension cells (MIC 20 mg/L of polydisperse AgNPs dispersed in water). Our results bring new insight into the economical production of AgNPs with defined characteristics, which were proven to target a specific mode of growth of significant pathogen C. albicans.


Subject(s)
Metal Nanoparticles , Silver , Anti-Bacterial Agents/pharmacology , Antifungal Agents/metabolism , Antifungal Agents/pharmacology , Biofilms , Candida albicans/metabolism , Microbial Sensitivity Tests , Plant Extracts/metabolism , Plant Extracts/pharmacology , Silver/metabolism , Silver/pharmacology , Water/metabolism
3.
Biotechnol Adv ; 58: 107905, 2022 09.
Article in English | MEDLINE | ID: mdl-35031394

ABSTRACT

There is a growing interest in the potential and application of metal nanoparticles across many fields. A vast array of techniques for metal nanoparticle synthesis has been discovered; however, sustainability, cost-effectiveness, and environmental concerns favor the green biological approach, using various plant and microbial sources. This review describes the diversity in green methods for nanoparticle biosynthesis, antimicrobial properties of metal nanoparticles and their potential applications. Metal nanoparticle biosynthesis by extracts and solutions obtained from plants, bacteria, fungi and templates such as viruses are discussed. As biosynthesized nanoparticles have been proven to possess antibacterial, antifungal, and even antiviral properties, these are discussed in detail, with silver and gold nanoparticles as the most studied and with the highest potential for medical application. The focus on prospective antimicrobial applications of nanoparticles stems from the arising resistance of many serious pathogens to traditional disinfectants and antibiotics. Other fields for the application of biosynthesized nanoparticles are also stated briefly, such as in agriculture as pesticides, in wastewater treatment and bioremediation. Finally, the limitations and safety issues connected with widespread use of nanoparticles are discussed.


Subject(s)
Anti-Infective Agents , Metal Nanoparticles , Anti-Bacterial Agents , Anti-Infective Agents/pharmacology , Gold , Green Chemistry Technology , Plant Extracts , Plants , Prospective Studies
4.
Folia Microbiol (Praha) ; 63(3): 261-272, 2018 May.
Article in English | MEDLINE | ID: mdl-28971316

ABSTRACT

Microbial adhesion to surfaces and the subsequent biofilm formation may result in contamination in food industry and in healthcare-associated infections and may significantly affect postoperative care. Some plants produce substances with antioxidant and antimicrobial properties that are able to inhibit the growth of food-borne pathogens. The aim of our study was to evaluate antimicrobial and anti-biofilm effect of baicalein, resveratrol, and pterostilbene on Candida albicans, Staphylococcus epidermidis, Pseudomonas aeruginosa, and Escherichia coli. We determined the minimum inhibitory concentrations (MIC), the minimum adhesion inhibitory concentration (MAIC), and the minimum biofilm eradication concentration (MBEC) by crystal violet and XTT determination. Resveratrol and pterostilbene have been shown to inhibit the formation of biofilms as well as to disrupt preformed biofilms. Our results suggest that resveratrol and pterostilbene appear potentially very useful to control and inhibit biofilm contaminations by Candida albicans, Staphylococcus epidermidis, and Escherichia coli in the food industry.


Subject(s)
Anti-Infective Agents/pharmacology , Biofilms/drug effects , Flavanones/pharmacology , Plant Extracts/pharmacology , Stilbenes/pharmacology , Biofilms/growth & development , Candida albicans/drug effects , Candida albicans/growth & development , Escherichia coli/drug effects , Escherichia coli/growth & development , Microbial Sensitivity Tests , Plant Extracts/chemistry , Pseudomonas aeruginosa/drug effects , Pseudomonas aeruginosa/growth & development , Resveratrol , Staphylococcus epidermidis/drug effects , Staphylococcus epidermidis/growth & development
5.
Article in English | MEDLINE | ID: mdl-14524696

ABSTRACT

A column reactor was designed and used to simulate conditions affecting the bioremediations of petroleum hydrocarbons. The work illustratively describes the aerobic (model) clean-up of soil samples enabling to predict the efficiency of a technology installed in parallel on contaminated former airport. The data showing the performance of thus precharacterized technology are presented.


Subject(s)
Hydrocarbons/isolation & purification , Petroleum , Soil Pollutants/isolation & purification , Water Pollutants/isolation & purification , Bacteria, Aerobic/physiology , Biodegradation, Environmental , Bioreactors
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