ABSTRACT
This research used batch soil column experiment to study the effects of irrigating with reclaimed water and tap water on the soil chemical properties and culturable microorganisms. The results indicated that reclaimed water could markedly increase the soil organic material (OM) and total nitrogen (TN) content, but it had no obvious effect on total phosphorus (TP), available phosphorus (AP) and pH value. Reclaimed water irrigation could significantly enhance the amounts of surface soil bacteria and actinomycetes at a depth of 0-20 cm, but it had little effect on the biomass of 20-40 cm and 40-60 cm soil layers. The dominant bacteria in tap water irrigation area was the genus Bacillus whereas that of reclaimed water irrigation area was the genus Acinetobacter. Tap water irrigation area had four endemic genera and reclaimed water irrigation area had six endemic genera. Reclaimed water had no obvious effect on the microbial community Shannon diversity of 0-20 cm soil layer, while it decreased Pielou evenness index, and improved Margalef richness index. Through SPSS 17. 0 correlation analysis between soil microbes quantity and soil chemical properties, it was shown that the soil microbes quantity was positively correlated with OM, TN, TP and AP, but negatively correlated with soil water content (SWC) and pH value. Based on CANOCO 4.5 detrended correspondence analysis (DCA) and redundancy analysis (RDA) between soil microbes species and soil chemical properties, it was shown that AP had the strongest correlation with the microbial community (P = 0.002). TN and TP had larger impact on Streptococcus, Aeromonas and Neisseria. OM and AP had larger impact on Aerococcus, Planococcus and Halobacterium.