ABSTRACT
OBJECTIVE: To investigate the effect of Sarcandra glabra Thunb on prevention and treatment of thrombocytopenia after chemotherapy with a large dosage. METHODS: The Babl/c mice model of thrombocytopenia was made by intraperitoneal injection of 5-FU at a large dosage. All mice were poured Sarcandra glabra Thunb dilution to the mice's stomach once daily from the beginning of the experiment. Then the effect was analyzed by measuring peripheral blood cell counts of the mice on the 2nd day, the 4th day and the 7th day after chemotherapy. On the end of 7th day, all mice were killed, and then bone marrow biopsy was employed to investigate the hemapoietic condition. RESULTS: Before the chemotherapy, the platelet counts of mice treated by Sarcandra glabra Thunb were higher than that in the control group (P < 0.05). After chemotherapy with 5-FU, the platelet counts were not significantly decreased in two all experimental groups compared with negative control group (P < 0.05), while there was not significantly different between two experimental groups (P > 0.05). Moreover the marrow biopsy showed that bone marrow hyperplasia was active in two intervention study groups with some megaryocytes in the medullary cavity of bone while bone marrow hyperplasia was low in the control group without any megaryocyte in the medullary cavity of bone. CONCLUSION: The Sarcandra glabra Thunb had obvious activity in treatment to therombocytopenia and can prevent thrombocytopenia by 5-FU.
Subject(s)
Drugs, Chinese Herbal/administration & dosage , Magnoliopsida/chemistry , Plants, Medicinal/chemistry , Thrombocytopenia/prevention & control , Animals , Antineoplastic Agents/adverse effects , Erythrocyte Count , Fluorouracil/administration & dosage , Hematopoiesis/drug effects , Male , Mice , Mice, Inbred BALB C , Platelet Count , Thrombocytopenia/blood , Thrombocytopenia/chemically inducedABSTRACT
OBJECTIVE: To investigate the possibility of the oil from Hippophae rhamnoides (OHR) in supporting the hematopoitic reconstitution after high dose chemotherapy. METHOD: The mice were fed with OHR and variation of blood cell counts of the mice was recorded. Animal models of myelosuppression were built by 5-Fu intra-peritoneal infusion. The effects of OHR in the hematopoietic reconstitution were observed. RESULTS: The counts of erythrocytes of OHR group were significantly elevated. The blood cell counts in myelosuppression mice fed with OHR exceeded those in control group, and the mortality was decreased. CONCLUSION: OHR can improve the hematopoiesis of erythroid linage. Like G-CSF, OHR can stimulate the recovery of hematopoiesis after chemotherapy.