ABSTRACT
Acetic acid-induced stress is a common challenge in natural environments and industrial bioprocesses, significantly affecting the growth and metabolic performance of Saccharomyces cerevisiae. The adaptive response and tolerance to this stress involves the activation of a complex network of molecular pathways. This study aims to delve deeper into these mechanisms in S. cerevisiae, particularly focusing on the role of the Hrk1 kinase. Hrk1 is a key determinant of acetic acid tolerance, belonging to the NPR/Hal family, whose members are implicated in the modulation of the activity of plasma membrane transporters that orchestrate nutrient uptake and ion homeostasis. The influence of Hrk1 on S. cerevisiae adaptation to acetic acid-induced stress was explored by employing a physiological approach based on previous phosphoproteomics analyses. The results from this study reflect the multifunctional roles of Hrk1 in maintaining proton and potassium homeostasis during different phases of acetic acid-stressed cultivation. Hrk1 is shown to play a role in the activation of plasma membrane H+-ATPase, maintaining pH homeostasis, and in the modulation of plasma membrane potential under acetic acid stressed cultivation. Potassium (K+) supplementation of the growth medium, particularly when provided at limiting concentrations, led to a notable improvement in acetic acid stress tolerance of the hrk1Δ strain. Moreover, abrogation of this kinase expression is shown to confer a physiological advantage to growth under K+ limitation also in the absence of acetic acid stress. The involvement of the alkali metal cation/H+ exchanger Nha1, another proposed molecular target of Hrk1, in improving yeast growth under K+ limitation or acetic acid stress, is proposed.
ABSTRACT
The ascomycetous yeast Candida membranifaciens has been isolated from diverse habitats, including humans, insects, and environmental sources, exhibiting a remarkable ability to use different carbon sources that include pentoses, melibiose, and inulin. In this study, we isolated four C. membranifaciens strains from soil and investigated their potential to overproduce riboflavin. C. membranifaciens IST 626 was found to produce the highest concentrations of riboflavin. The volumetric production of this vitamin was higher when C. membranifaciens IST 626 cells were cultured in a commercial medium without iron and when xylose was the available carbon source compared to the same basal medium with glucose. Supplementation of the growth medium with 2 g/L glycine favored the metabolization of xylose, leading to biomass increase and consequent enhancement of riboflavin volumetric production that reached 120 mg/L after 216 h of cultivation. To gain new insights into the molecular basis of riboflavin production and carbon source utilization in this species, the first annotated genome sequence of C. membranifaciens is reported in this article, as well as the result of a comparative genomic analysis with other relevant yeast species. A total of 5619 genes were predicted to be present in C. membranifaciens IST 626 genome sequence (11.5 Mbp). Among them are genes involved in riboflavin biosynthesis, iron homeostasis, and sugar uptake and metabolism. This work put forward C. membranifaciens IST 626 as a riboflavin overproducer and provides valuable molecular data for future development of superior producing strains capable of using the wide range of carbon sources, which is a characteristic trait of the species.
ABSTRACT
Pectin-rich plant biomass residues represent underutilized feedstocks for industrial biotechnology. The conversion of the oxidized monomer d-galacturonic acid (d-GalUA) to highly reduced fermentation products such as alcohols is impossible due to the lack of electrons. The reduced compound glycerol has therefore been considered an optimal co-substrate, and a cell factory able to efficiently co-ferment these two carbon sources is in demand. Here, we inserted the fungal d-GalUA pathway in a strain of the yeast S. cerevisiae previously equipped with an NAD-dependent glycerol catabolic pathway. The constructed strain was able to consume d-GalUA with the highest reported maximum specific rate of 0.23 g gCDW-1 h-1 in synthetic minimal medium when glycerol was added. By means of a 13C isotope-labelling analysis, carbon from both substrates was shown to end up in pyruvate. The study delivers the proof of concept for a co-fermentation of the two 'respiratory' carbon sources to ethanol and demonstrates a fast and complete consumption of d-GalUA in crude sugar beet pulp hydrolysate under aerobic conditions. The future challenge will be to achieve co-fermentation under industrial, quasi-anaerobic conditions.
Subject(s)
Glycerol , Saccharomyces cerevisiae , Fermentation , Glycerol/metabolism , Hexuronic Acids , Pectins/genetics , Pectins/metabolism , Saccharomyces cerevisiae/genetics , Saccharomyces cerevisiae/metabolismABSTRACT
Agro-industrial residues are low-cost carbon sources (C-sources) for microbial growth and production of value-added bioproducts. Among the agro-industrial residues available, those rich in pectin are generated in high amounts worldwide from the sugar industry or the industrial processing of fruits and vegetables. Sugar beet pulp (SBP) hydrolysates contain predominantly the neutral sugars d-glucose, l-arabinose and d-galactose, and the acidic sugar d-galacturonic acid. Acetic acid is also present at significant concentrations since the d-galacturonic acid residues are acetylated. In this study, we have examined and optimized the performance of a Rhodotorula mucilaginosa strain, isolated from SBP and identified at the molecular level during this work. This study was extended to another oleaginous red yeast species, R. toruloides, envisaging the full utilization of the C-sources from SBP hydrolysate (at pH 5.0). The dual role of acetic acid as a carbon and energy source and as a growth and metabolism inhibitor was examined. Acetic acid prevented the catabolism of d-galacturonic acid and l-arabinose after the complete use of the other C-sources. However, d-glucose and acetic acid were simultaneously and efficiently metabolized, followed by d-galactose. SBP hydrolysate supplementation with amino acids was crucial to allow d-galacturonic acid and l-arabinose catabolism. SBP valorization through the production of lipids and carotenoids by Rhodotorula strains, supported by complete catabolism of the major C-sources present, looks promising for industrial implementation.
ABSTRACT
Pectin-rich agro-industrial residues are feedstocks with potential for sustainable biorefineries. They are generated in high amounts worldwide from the industrial processing of fruits and vegetables. The challenges posed to the industrial implementation of efficient bioprocesses are however manyfold and thoroughly discussed in this review paper, mainly at the biological level. The most important yeast cell factory platform for advanced biorefineries is currently Saccharomyces cerevisiae, but this yeast species cannot naturally catabolise the main sugars present in pectin-rich agro-industrial residues hydrolysates, in particular D-galacturonic acid and L-arabinose. However, there are non-Saccharomyces species (non-conventional yeasts) considered advantageous alternatives whenever they can express highly interesting metabolic pathways, natively assimilate a wider range of carbon sources or exhibit higher tolerance to relevant bioprocess-related stresses. For this reason, the interest in non-conventional yeasts for biomass-based biorefineries is gaining momentum. This review paper focuses on the valorisation of pectin-rich residues by exploring the potential of yeasts that exhibit vast metabolic versatility for the efficient use of the carbon substrates present in their hydrolysates and high robustness to cope with the multiple stresses encountered. The major challenges and the progresses made related with the isolation, selection, sugar catabolism, metabolic engineering and use of non-conventional yeasts and S. cerevisiae-derived strains for the bioconversion of pectin-rich residue hydrolysates are discussed. The reported examples of value-added products synthesised by different yeasts using pectin-rich residues are reviewed. Key Points ⢠Review of the challenges and progresses made on the bioconversion of pectin-rich residues by yeasts. ⢠Catabolic pathways for the main carbon sources present in pectin-rich residues hydrolysates. ⢠Multiple stresses with potential to affect bioconversion productivity. ⢠Yeast metabolic engineering to improve pectin-rich residues bioconversion. Graphical abstract.
Subject(s)
Agriculture , Industrial Waste , Metabolic Engineering , Pectins/metabolism , Yeasts/metabolism , Biomass , Carbon/metabolism , Ethanol/metabolism , Fermentation , Metabolic Networks and Pathways , Pectins/analysis , Sugars/metabolism , Yeasts/classificationABSTRACT
Allergic diseases are a major health concern worldwide. Pollens are important triggers for allergic rhinitis, conjunctivitis and asthma. Proteases released upon pollen grain hydration appear to play a major role in the typical immunological and inflammatory responses that occur in patients with allergic disorders. In this study, we aimed to identify specific proteolytic activity in a set of pollens with diverse allergenic potential. Diffusates from Chenopodium album, Plantago lanceolata and Eucalyptus globulus were added to a confluent monolayer of Calu-3 cells grown in an air-liquid interface system. We identified serine proteases and metalloproteinases in all pollen diffusates investigated. Proteases found in these pollen diffusates were shown to compromise the integrity of the lung epithelial barrier by disrupting transmembrane adhesion proteins E-cadherin, claudin-1 and Occludin, as well as, the cytosolic complex zonula occludens-1 (ZO-1) resulting in a time-dependent increase in transepithelial permeability. Tight junction disruption and increased transepithelial permeability facilitates allergen exposure to epithelial sub-layers contributing to the sensitization to a wide range of allergens. These pollen extracts also induced an increase in the release of interleukin 6 (IL-6) and interleukin 8 (IL-8) cytokines measured by flow cytometry possibly as a result of the activation of protease-activated receptors 2 (PAR-2).
Subject(s)
Hypersensitivity/enzymology , Peptide Hydrolases/metabolism , Pollen/enzymology , Cell Line , Chenopodium/enzymology , Eucalyptus/enzymology , Humans , Interleukin-6/metabolism , Interleukin-8/metabolism , Plantago/enzymology , Receptor, PAR-2/metabolism , WaterABSTRACT
Objetivo: Compreender a extensão dos componentes do Método Clínico Centrado na Pessoa na prática da acupuntura a partir da percepção dos usuários. Métodos: Por meio de uma pesquisa qualitativa com grupo focal, buscou-se entender como a acupuntura faz a pessoa perceber suas experiências de saúde e doença; intensifica a percepção da pessoa enquanto ser integral; viabiliza a pessoa se sentir participante do processo de cuidado; auxilia na intensificação da relação médico-pessoa. Resultados: Emergiram temas que versavam sobre emancipação, autoconhecimento, integralidade, vínculos e afetos, que foram sistematizados nas categorias: A prática integrada do MCCP/MTC produzindo rizomas; Unidade mente-corpo no contexto do cuidado; Percepção de uma anatomia emocional e MCCP E MTC como reconfiguradoras do Circuito dos Afetos. Conclusões: O estudo reflete, a partir da percepção dos usuários, a utilização dos conhecimentos advindos de outras racionalidades para potencializar o cuidado realizado na Atenção Primária à Saúde.
Objective: To understand the extent of the components of the Person-Centered Clinical Method relates to the practice of acupuncture, based on the perception of the users. Methods: By a qualitative research with focus group, this study aimed to understand how acupuncture makes the person to perceive their health and illness experience; intensifies the person's perception as an integral being; allows for the person to participate in the process of care; strengths the physician-person relationship. Results: The themes that emerged included emancipation, self-knowledge, completeness, bonds and affections. They were organized in categories: Integrated MCCP/MTC practice producing rhizomes; Mind-body unity in the context of care; Perception of an emotional anatomy and MCCP and MTC reconfiguring the Circuit of Affections. Conclusions: The study reflects, from the perception of the users, the use of the knowledge that derives from other rationalities to enhance the care performed in Primary Health Care.
Objetivo: Comprender la extensión de los componentes del Método Clínico Centrado en la Persona en la práctica de la acupuntura, a partir de la percepción de los usuarios. Métodos: A través de una investigación cualitativa con grupo focal, buscamos comprender cómo la acupuntura hace que la persona perciba sus experiencias de salud y enfermedad; Intensifica la percepción de la persona como un ser integral; Permite que la persona se sienta involucrada en el proceso de cuidados; Esto ayuda a intensificar la relación médico-persona. Resultados: Surgieron temas relacionados a la emancipación, autoconocimiento, integralidad, vínculos y afecciones, que se sistematizaron en las categorías: práctica integrada MCCP/TCM produciendo rizomas; Unidad mente-cuerpo en el contexto del cuidado; Percepción de una anatomía emocional y MCCP y MTC como nuevos configuradores del Circuito de Afectos. Conclusiones: El estudio refleja, a partir de la percepción de los usuarios, el uso de conocimientos advenidos de otras racionalidades para mejorar el cuidado realizado en la Atención Primaria a la Salud.
Subject(s)
Humans , Acupuncture Therapy , Patient-Centered Care , Family PracticeABSTRACT
The abietane 7α-acetoxy-6ß-hydroxyroyleanone (AHR), obtained from plant extracts, is an attractive lead for drug development, given its known antimicrobial properties. Two basic requirements to establish any compound as a new drug are the development of a convenient extraction process and the characterization of its structural and thermal properties. In this work seven different methods were tested to optimize the extraction of AHR from Plectranthus grandidentatus. Supercritical fluid extraction (SFE) proved to be the method of choice, delivering an amount of AHR (57.351 µg·mg-1) approximately six times higher than the second best method (maceration in acetone; 9.77 µg·mg-1). Single crystal X-ray diffraction analysis of the ARH molecular and crystal structure carried out at 167 ± 2 K and 296 ± 2 K showed only a single phase, here dubbed form III (orthorhombic space group P21212), at those temperatures. The presence of two other polymorphs above room temperature was, however, evidenced by differential scanning calorimetry (DSC). The three forms are enantiotropically related, with the form III â form II and form II â form I transitions occurring at 333.5 ± 1.6 K and 352.0 ± 1.6 K, respectively. The fact that the transitions are reversible suggests that polymorphism is not likely to be an issue in the development pharmaceutical formulations based on ARH. DSC experiments also showed that the compound decomposes on melting at 500.8 ± 0.8 K. Melting should therefore be avoided if, for example, strategies to improve solubility based on the production of glassy materials or solid dispersions are considered.
Subject(s)
Abietanes/chemistry , Anti-Bacterial Agents/chemistry , Plant Extracts/chemistry , Calorimetry, Differential Scanning/methods , Chemistry, Pharmaceutical/methods , Crystallization/methods , Crystallography, X-Ray/methods , Plectranthus/chemistry , Solubility , Temperature , X-Ray Diffraction/methodsABSTRACT
The application of functionalized nanocarriers on photothermal therapy for cancer ablation has wide interest. The success of this application depends on the therapeutic efficiency and biocompatibility of the system, but also on the stability and biorecognition of the conjugated protein. This study aims at investigating the hypothesis that EGF functionalized polymer-coated gold nanoparticles promote EGF photostability and EGFR internalization, making these conjugated particles suitable for photothermal therapy. The conjugated gold nanoparticles (100-200 nm) showed a plasmon absorption band located within the near-infrared range (650-900 nm), optimal for photothermal therapy applications. The effects of temperature, of polymer-coated gold nanoparticles and of UVB light (295nm) on the fluorescence properties of EGF have been investigated with steady-state and time-resolved fluorescence spectroscopy. The fluorescence properties of EGF, including the formation of Trp and Tyr photoproducts, is modulated by temperature and by the intensity of the excitation light. The presence of polymeric-coated gold nanoparticles reduced or even avoided the formation of Trp and Tyr photoproducts when EGF is exposed to UVB light, protecting this way the structure and function of EGF. Cytotoxicity studies of conjugated nanoparticles carried out in normal-like human keratinocytes showed small, concentration dependent decreases in cell viability (0-25%). Moreover, conjugated nanoparticles could activate and induce the internalization of overexpressed Epidermal Growth Factor Receptor in human lung carcinoma cells. In conclusion, the gold nanoparticles conjugated with Epidermal Growth Factor and coated with biopolymers developed in this work, show a potential application for near infrared photothermal therapy, which may efficiently destroy solid tumours, reducing the damage of the healthy tissue.
Subject(s)
Epidermal Growth Factor/chemistry , Epidermal Growth Factor/pharmacology , ErbB Receptors/metabolism , Gold/chemistry , Metal Nanoparticles/chemistry , Phototherapy , Polymers/chemistry , A549 Cells , Cell Survival/drug effects , Drug Carriers/chemistry , Drug Carriers/toxicity , Gold/toxicity , Humans , Hyaluronic Acid/chemistry , Light , Oleic Acid/chemistry , Protein Stability/radiation effects , Protein Transport/drug effects , Protein Transport/radiation effects , TemperatureABSTRACT
The presence of toxic compounds derived from biomass pre-treatment in fermentation media represents an important drawback in second-generation bio-ethanol production technology and overcoming this inhibitory effect is one of the fundamental challenges to its industrial production. The aim of this study was to systematically identify, in industrial medium and at a genomic scale, the Saccharomyces cerevisiae genes required for simultaneous and maximal tolerance to key inhibitors of lignocellulosic fermentations. Based on the screening of EUROSCARF haploid mutant collection, 242 and 216 determinants of tolerance to inhibitory compounds present in industrial wheat straw hydrolysate (WSH) and in inhibitor-supplemented synthetic hydrolysate were identified, respectively. Genes associated to vitamin metabolism, mitochondrial and peroxisomal functions, ribosome biogenesis and microtubule biogenesis and dynamics are among the newly found determinants of WSH resistance. Moreover, PRS3, VMA8, ERG2, RAV1 and RPB4 were confirmed as key genes on yeast tolerance and fermentation of industrial WSH.
Subject(s)
Genes, Fungal , Industrial Microbiology , Lignin/metabolism , Saccharomyces cerevisiae/genetics , Acetic Acid/toxicity , Biomass , Drug Resistance, Fungal , Fermentation , Furaldehyde/toxicity , Genome, Fungal , Saccharomyces cerevisiae/drug effects , Saccharomyces cerevisiae/metabolism , Stress, Physiological/genetics , TriticumABSTRACT
Recent reports suggest that N-methyl-d-aspartate receptor (NMDAR) blockade by MK-801 decreases tumor growth. Thus, we investigated whether other ionotropic glutamate receptor (iGluR) antagonists were also able to modulate the proliferation of melanoma cells. On the other hand, the antiestrogen tamoxifen (TAM) decreases the proliferation of melanoma cells, and is included in combined therapies for melanoma. As the efficacy of TAM is limited by its metabolism, we investigated the effects of the NMDAR antagonist MK-801 in combination with TAM and its active metabolites, 4-hydroxytamoxifen (OHTAM) and endoxifen (EDX). The NMDAR blockers MK-801 and memantine decreased mouse melanoma K1735-M2 cell proliferation. In contrast, the NMDAR competitive antagonist APV and the AMPA and kainate receptor antagonist NBQX did not affect cell proliferation, suggesting that among the iGluR antagonists only the NMDAR channel blockers inhibit melanoma cell proliferation. The combination of antiestrogens with MK-801 potentiated their individual effects on cell biomass due to diminished cell proliferation, since it decreased the cell number and DNA synthesis without increasing cell death. Importantly, TAM metabolites combined with MK-801 promoted cell cycle arrest in G1. Therefore, the data obtained suggest that the activity of MK-801 and antiestrogens in K1735-M2 cells is greatly enhanced when used in combination.
Subject(s)
Antineoplastic Agents, Hormonal/pharmacology , Cell Proliferation/drug effects , Dizocilpine Maleate/pharmacology , Excitatory Amino Acid Antagonists/pharmacology , Melanoma/pathology , Tamoxifen/pharmacology , Animals , Drug Evaluation, Preclinical , Drug Therapy, Combination , Melanoma/drug therapy , Mice , Tamoxifen/analogs & derivatives , Tamoxifen/metabolism , Tumor Cells, CulturedABSTRACT
Chronic respiratory infections caused by Burkholderia cenocepacia in patients with cystic fibrosis (CF) are characterized by low responsiveness to antibiotic therapy and, in general, to a more rapid decline of lung function. To get clues into the molecular mechanisms underlying the adaptive strategies employed to deal with the stressing conditions of the CF lung including antibiotic therapy, quantitative proteomics (2-D DIGE) was used to compare the expression programs of two clonal isolates retrieved from a chronically infected CF patient. Isolate IST439 was the first bacterium recovered while the clonal variant IST4113 was obtained after 3 years of persistent infection and intravenous therapy with ceftazidime/gentamicin. This isolate exhibits higher resistance levels towards different classes of antimicrobials. Proteins of the functional categories Energy metabolism, Translation, Nucleotide synthesis, Protein folding and stabilization are more abundant in IST4113, compared with IST439, suggesting an increased protein synthesis, DNA repair and stress resistance in IST4113. The level of proteins involved in peptidoglycan, membrane lipids and lipopolysaccharide synthesis is also altered and proteins involved in iron binding and transport are more abundant in IST4113. The quantitative comparison of the two proteomes suggests a genetic adaptation leading to increased antimicrobial resistance and bacterial persistence in the CF airways.
Subject(s)
Bacterial Proteins/metabolism , Burkholderia cenocepacia/drug effects , Burkholderia cenocepacia/genetics , Proteomics , Adaptation, Physiological/drug effects , Adaptation, Physiological/genetics , Anti-Bacterial Agents/administration & dosage , Anti-Bacterial Agents/therapeutic use , Bacterial Proteins/genetics , Burkholderia Infections/drug therapy , Burkholderia Infections/microbiology , Burkholderia Infections/physiopathology , Burkholderia cenocepacia/isolation & purification , Burkholderia cenocepacia/metabolism , Ceftazidime/administration & dosage , Ceftazidime/therapeutic use , Cell Culture Techniques , Cystic Fibrosis/drug therapy , Cystic Fibrosis/microbiology , Cystic Fibrosis/physiopathology , DNA Repair Enzymes/genetics , DNA Repair Enzymes/metabolism , Drug Resistance/drug effects , Drug Resistance/genetics , Electrophoresis, Gel, Two-Dimensional , Gene Expression/drug effects , Gentamicins/administration & dosage , Gentamicins/therapeutic use , Heat-Shock Proteins/genetics , Heat-Shock Proteins/metabolism , Humans , Iron-Binding Proteins/genetics , Iron-Binding Proteins/metabolism , Microbial Sensitivity Tests , Respiratory System/microbiology , Respiratory System/physiopathology , Reverse Transcriptase Polymerase Chain Reaction , Up-RegulationABSTRACT
BACKGROUND: The first detailed chemical characterisation of Portuguese pine nut (Pinus pinea L.) is reported concerning proximate composition, fatty acid, mineral and vitamin contents. RESULTS: Based on the analysis of 27 different populations, pine nuts were characterised by high contents of fat (47.7 g per 100 g dry matter (DM)), protein (33.8 g per 100 g DM) and phosphorus (1130 mg per 100 g DM) and low contents of moisture (5.9 g per 100 g DM) and starch (3.5 g per 100 g DM). They were also found to be a good source of zinc, iron and manganese. CONCLUSION: Mineral composition seemed to be most prone to variation, suggesting its potentially useful role in discriminating Mediterranean pine nuts. A significant variability was found in the nut composition of Portuguese P. pinea populations.
Subject(s)
Nutritive Value , Pinus/chemistry , Seeds/chemistry , Fatty Acids/analysis , Minerals/analysis , Nuts , Phosphorus/analysis , Pinus/genetics , Plant Oils/analysis , Plant Proteins/analysis , Portugal , Starch/analysis , Trace Elements/analysis , Vitamins/analysis , Water/analysisABSTRACT
Solar urticaria is an idiopathic, chronic and rare photodermatosis, characterized by the sudden onset of pruritic urticarial hives and plaques on the exposed areas of the skin, after a brief period of exposure to the natural sunlight or to an artificial light source. A Caucasian 27-year-old man presented with clinical features suggestive of solar urticaria was referred to our photodermatology unit, where phototesting confirmed the diagnosis of solar urticaria induced by visible light. As he was refractory to oral antihistamines and had slight improvement under UVA plus visible phototherapy, human high-dose intravenous immunoglobulin was administered, with an excellent clinical-sustained response.
Subject(s)
Immunoglobulins, Intravenous/immunology , Immunoglobulins, Intravenous/therapeutic use , Immunotherapy , Skin/radiation effects , Urticaria/immunology , Urticaria/therapy , Adult , Humans , Immunoglobulins, Intravenous/administration & dosage , MaleABSTRACT
Two different test systems, one based on the isolated sciatic nerve of an amphibian and the other on a microbial eukaryote, were used for the assessment of herbicide toxicity. More specifically, we determined the deleterious effects of increasing concentrations of herbicides of different chemical classes (phenoxyacetic acids, triazines, and acetamides), and of 2,4-dichlorophenol (2,4-DCP), a degradation product of the herbicide 2,4-dichlorophenoxyacetic acid (2,4-D), on electrophysiological parameters and the vitality of the axons of the isolated sciatic nerve of the frog (Rana ridibunda) and on the growth curve of the yeast Saccharomyces cerevisiae based on microtiter plate susceptibility assays. The no-observed-effect-concentration (NOEC), defined as the maximum concentration of the tested compound that has no effect on these biological parameters, was estimated. In spite of the different methodological approaches and biological systems compared, the NOEC values were identical and correlated with the lipophilicity of the tested compounds. The relative toxicity established here, 2,4-DCP > alachlor, metolachlor >> metribuzin > 2,4-D, 2-methyl-4-chlorophenoxyacetic acid (MCPA), correlates with the toxicity indexes reported in the literature for freshwater organisms. Based on these results, we suggest that the relatively simple, rapid, and low-cost test systems examined here may be of interest as alternative or complementary tests for toxicological assessment of herbicides.
Subject(s)
Biological Assay/methods , Herbicides/toxicity , Sciatic Nerve/drug effects , Yeasts/drug effects , 2,4-Dichlorophenoxyacetic Acid/toxicity , Acetamides/toxicity , Animals , Anura , Chlorophenols/toxicity , Saccharomyces cerevisiae/cytology , Saccharomyces cerevisiae/growth & development , Sciatic Nerve/metabolism , Time Factors , Triazines/toxicity , Yeasts/cytology , Yeasts/growth & developmentABSTRACT
Two Gram-negative bacterial strains capable of using lupanine, the predominant quinolizidine alkaloid in Lupinus albus, as a sole carbon source were isolated from soil in which L. albus and L. luteus had been grown [Santana, F. M. et al. J. Ind. Microbiol. 1996, 17, 110-115]. In the present study, we present results suggesting that these isolates are of potential interest for removing lupanine and other quinolizidine alkaloids (QA) from the effluent resulting from the wet processing of Lupinus seeds, at temperatures within the range 20-34 degrees C. Growth in L. albus aqueous extract was diauxic, with a first period of rapid growth leading to the simultaneous consumption of a significant part of the initial concentration of QA (3 g L(-1), being 2 g L(-1) lupanine) and amino acids (1.5 g L(-1)). This period was followed by a second period of slower growth corresponding to the subsequent partial utilization (25%) of the carbohydrates (initial concentration of 20 g L(-1)) together with further removal of QA and amino acids. Despite the differences detected in the susceptibility of the two strains to lupanine toxicity, in particular at supraoptimal temperatures, and in the efficiency of lupanine catabolism, their performance on L. albus extract did not vary significantly.