Damage of anodic biofilms by high salinity deteriorates PAHs degradation in single-chamber microbial electrolysis cell reactor.

The anaerobic biodegradation of polycyclic aromatic hydrocarbons (PAHs) in high salinity wastewater is rather hard due to the inhibition of microorganisms by complex and high dosage of salts. Microbial electrolysis cell (MEC), with its excellent characteristic of anodic biofilms, can be an effective way to enhance the PAHs biodegradation. This work evaluated the impact of NaCl concentrations (0 g/L, 10 g/L, 30 g/L, and 60 g/L) on naphthalene biodegradation and analyzed the damage protection mechanism of anodic biofilms in batching MECs. Compared with the open circuit, the degradation efficiency of naphthalene under the closed circuit with 10 g/L NaCl concentration reached the maximum of 95.17% within 5 days. Even when NaCl concentration reached 60 g/L, the degradation efficiency only decreased by 10.02%, compared with the MEC without additional NaCl. Confocal scanning laser microscope (CSLM) proved the superiority of the biofilm states of MEC anode under high salinity in terms of thicker biofilms and higher proportion of live/dead bacteria cells. The highest dehydrogenase activity (DHA) was found in the MEC with 10 g/L NaCl concentration. Moreover, microbial diversity analysis demonstrated the classical electroactive microorganisms Geobacter and Pseudomonas were found on the anodic biofilms of MECs, which have both PAHs degradability and the electrochemical activity. Therefore, this study proved that high salinity had adverse effects on the anodic biofilms, but MEC alleviated the damage caused by high salinity.

Mixed dye wastewater treatment in a bioelectrochemical system-centered process.

The feasibility of mixed dye wastewater treatment was evaluated with a novel integrated bioprocess that consisted of a hybrid anaerobic reactor (HAR) with a built-in bioelectrochemical system, an aerobic biofilm reactor (ABFR) and a denitrification reactor (DR). The position of the DR significantly affected chemical oxygen demand (COD) and colority in effluent, and placing the DR after the ABFR improved effluent quality probably due to minimization of the undesired autoxidation of aromatic amine in dye wastewater. The optimal integrated process of HAR + ABFR + DR efficiently treated mixed dye wastewater, and concentrations of COD and TN were decreased down to 75 ± 18 mg/L and 12.91 ± 0.31 mg/L, respectively, along with colority 48 ± 4 times. Total phosphorus reduced to below 0.5 mg/L with coagulation using poly aluminum chloride, and the effluent quality fully met the discharge standard. This comprehensive study suggests the feasibility of the BES based process for practical application to mixed dye wastewater treatment.