ABSTRACT
Twelve compounds were isolated from Liquidambaris Resina by silica gel column chromatography and thin layer chromatography. Their structures were identified on the basis of spectral data, electron capture detector data, and physicochemical properties as(2'R, 3'R)-2',3'-dihydroxy-hydrocinnamyl-(E)-cinnamate(1),(E)-cinnamyl-(E)-cinnamate(2), cinnamic acid(3), 28-norlup-20(29)-en-3-one-17ß-hydroperoxide(4), erythrodiol(5), 13ß,28-epoxy-30-hydroxyolean-1-en-3-one(6),(3ß)-olean-12-ene-3,23-diol(7), 2α,3α-dihydroxy-olean-12-en-28-oic acid(8), 28-hydroxyolean-12-en-3-one(9), 3-epi-oleanolic acid(10), 3-oxo-oleanolic acid(11), and hederagenin(12). Compound 1 was a new cinnamic acid ester derivative and compounds 2-4,6-8, and 12 were isolated from Liquidambaris Resina for the first time. Compounds 4, 5, 10, and 12 exerted inhibitory effects on the proliferation of human umbilical vein endothelial cells(HUVEC) with the IC_(50) values of(17.43±2.17),(35.32±0.61),(27.50±0.80), and(46.30±0.30) µmol·L~(-1), respectively.
Subject(s)
Oleanolic Acid , Triterpenes , Humans , Endothelial Cells , Esters , Cinnamates , Triterpenes/chemistry , Molecular StructureABSTRACT
OBJECTIVE: To investigate protective effect of total flavonoids of Hedysarum polybotry on human umbilical vein endothelial cells injury induced by hydrogen peroxide. METHODS: Chemical light assay was used to detect the oxygen radicals scavenging capacity of total flavonoids of Hedysarum polybotry. Lactate dehydrogenase (LDH) in the culture medium, malondialdelyde (MDA) and superoxide dismutase (SOD) in the cell were measured by spectroscopy, respectively. The degress of DNA damage was evaluated with single cell gel electrophoresis (SCGE) technique. RESULTS: Total flavonoids of Hedysarum polybortry could scavenge oxygen radicals. After being exposed to 100 micromol/L H2O2 for 4 hours, the level of LDH and MDA was increased significantly while SOD level was decreased. However, total flavonoids of Hedysarum polybotry could decrease the content of LDH, MDA and increase SOD activity significantly and inhibit the damage of DNA. CONCLUSION: Total flavonoids of Hedysarum polybotry has protective effect on human umbilical vein endothelial cells injury induced by hydrogen peroxide,which may be related to increasing the antioxidant capacity of human umbilical vein endothelial cells.
Subject(s)
Antioxidants/pharmacology , Endothelial Cells/drug effects , Fabaceae/chemistry , Flavonoids/pharmacology , Cells, Cultured , Comet Assay , DNA Damage , Drugs, Chinese Herbal/pharmacology , Endothelial Cells/metabolism , Endothelial Cells/pathology , Humans , Hydrogen Peroxide/pharmacology , L-Lactate Dehydrogenase/metabolism , Malondialdehyde/metabolism , Plants, Medicinal/chemistry , Superoxide Dismutase/metabolism , Umbilical Veins/cytologyABSTRACT
OBJECTIVE: To study the constituents of Hypericum attenatum. METHOD: The compounds were isolated by chromatography on silica gel, the structures were identified by their physical, chemical properties and IR, NMR and MS spectral data respectively. RESULT: Nine compounds were isolated and identified as p-hydroxybenzoic acid (1), 6, 9-dihydroxy-4, 7-megastigmadien-3-one (2), butyl alcohol-O-alpha-D-fructoside (3), 24-ethyl-cholest-7-ene-3 beta, 5 alpha, 6 beta-thtroil (4), hexanol (5), 1 beta, 6 alpha-dihydroxyeudesmane-4(14)-ene (6), beta-sitosterol (7), 5, 5-dimethyl-4-hydroxy-tetrahydrofuran-2-one (8), beta-daucosterol (9). CONCLUSION: All of the compounds were isolated from H. attenuatum for the first time.