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[Experimental study on effect of total flavone of Ginkgo biloba on delaying cell senescence].

Song, Xiao-ping; Chen, Zhi-wu; Fang, An-ning; Fang, Pei-fei.

Zhong Yao Cai ; 32(1): 100-2, 2009 Jan.

Article in Chinese | MEDLINE | ID: mdl-19445133

ABSTRACT

OBJECTIVE: To investigate the mechanism of delaying the senescence of human diploid fibroblast (2BS) by total flavone of Ginkgo biloba (FG). METHODS: The drug sera of FG was used to treat the 2BS. The population doublings of 2BS cells were observed, the mRNA expression of P16 gene was determined by fluorescence real-time quantitative PCR. RESULTS: FG significantly extended the population doublings of 2BS cells, and decreased the expression of P16 mRNA. CONCLUSION: FG can delay the senescence of cells by inhibiting the P16 gene expression.

Subject(s)

Antioxidants/pharmacology , Cellular Senescence/drug effects , Cyclin-Dependent Kinase Inhibitor p16/metabolism , Flavones/pharmacology , Ginkgo biloba/chemistry , Animals , Cell Cycle/drug effects , Cells, Cultured , Gene Expression Regulation , Humans , Male , Plant Leaves/chemistry , Plants, Medicinal/chemistry , Polymerase Chain Reaction , RNA, Messenger/metabolism , Rats

ABSTRACT

Subject(s)

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