ABSTRACT
BACKGROUND AND AIM: An example of a medicinal plant with numerous potential activities is fenugreek (Trigonella foenum-graecum L.). The aim of the present study was to investigate the effects of fenugreek seed on bone mechanical properties in rats with normal and decreased estrogen level (developing osteoporosis). MATERIALS AND METHODS: The experiments were carried out on 3-month-old non-ovariectomized (NOVX) and ovariectomized (OVX) Wistar rats, divided into control rats, rats receiving pulverized fenugreek seed (1% in the diet) and rats receiving fenugreek seed extract standardized for 4-hydroxy-L-isoleucine (50 mg of 4-hydroxy-L-isoleucine/kg p.o. daily) for 4 weeks. Serum bone turnover markers, bone mineralization and mechanical properties were examined. RESULTS: Fenugreek seed added to food did not significantly affect bone mineralization and serum turnover markers, independently of the estrogen status. It tended to increase the strength of the tibial metaphysis (cancellous bone) in NOVX rats, and increased the strength of the femoral diaphysis (compact bone) in OVX rats. The fenugreek seed extract did not affect the skeletal system of NOVX rats, and significantly worsened mineralization of the vertebra in OVX rats, decreased due to estrogen deficiency. CONCLUSIONS: Low dietary intake of fenugreek seed may exert slight favorable skeletal effects, whereas at high doses it may damage the skeletal system.
Subject(s)
Femur/drug effects , Plant Extracts/pharmacology , Tibia/drug effects , Animals , Biomechanical Phenomena , Body Weight/drug effects , Calcification, Physiologic/drug effects , Diet , Dose-Response Relationship, Drug , Estradiol/blood , Estradiol/pharmacology , Female , Femur/physiology , Ovariectomy , Rats, Wistar , Seeds , Stress, Mechanical , Tibia/physiology , TrigonellaABSTRACT
The gene encoding the coat protein (CP) of a potato virus Y (PVY) was cloned into expression vector pMPM-A4Omega. PVY CP was expressed in Escherichia coli and the purified recombinant protein was used for raising rabbit polyclonal antibodies. The sera and antibodies were tested for the detection of PVY in the laboratory host Nicotiana tabacum cv. Petit Havana SR1 and in various cultivars of the natural host Solanum tuberosum by ELISA as well as by Western blots. The antibodies can be used for the detection of the whole strain spectrum of PVY by indirect plate trapped antigen ELISA and Western blot, but not by double antigen sandwich ELISA.