Employing Piper longum extract for eco-friendly fabrication of PtPd alloy nanoclusters: advancing electrolytic performance of formic acid and methanol oxidation.

Advancement in bioinspired alloy nanomaterials has a crucial impact on fuel cell applications. Here, we report the synthesis of PtPd alloy nanoclusters via the hydrothermal method using Piper longum extract, representing a novel and environmentally friendly approach. Physicochemical characteristics of the synthesized nanoclusters were investigated using various instrumentation techniques, including X-ray photoelectron spectroscopy, X-ray diffraction, and High-Resolution Transmission electron microscopy. The electrocatalytic activity of the biogenic PtPd nanoclusters towards the oxidation of formic acid and methanol was evaluated chronoamperometry and cyclic voltammetry studies. The surface area of the electrocatalyst was determined to be 36.6 m2g-1 by Electrochemical Surface Area (ECSA) analysis. The biologically inspired PtPd alloy nanoclusters exhibited significantly higher electrocatalytic activity compared to commercial Pt/C, with specific current responses of 0.24 mA cm - 2 and 0.17 mA cm - 2 at synthesis temperatures of 180 °C and 200 °C, respectively, representing approximately four times higher oxidation current after 120 min. This innovative synthesis approach offers a promising pathway for the development of PtPd alloy nanoclusters with enhanced electrocatalytic activity, thereby advancing fuel cell technology towards a sustainable energy solution.

Eggshells biowaste for hydroxyapatite green synthesis using extract piper betel leaf - Evaluation of antibacterial and antibiofilm activity.

The present research work reports the biosynthesis of hydroxyapatite (HAp) from eggshells and green synthesis of HAp from eggshells with incorporation of Piper betel leaf extract (PBL-HAp) using microwave conversion method. Although there are several works on synthesis of HAp from eggshells and other calcium and phosphorus rich substrates, the incorporation of herbal extract with HAp to promote antimicrobial and antibiofilm activity is less explored and reported. This research work highlights a simple and cost-effective method for development of antimicrobial biomaterials by combining the concepts of waste management, biomaterial science, and herbal medicine. In the present study, characterization of synthesized HAp was applied by X-ray Diffraction (XRD), Fourier Transform Infrared (FTIR) spectroscopy, Proton Nuclear Magnetic Resonance (1H NMR) spectroscopy, and morphological analysis using Scanning Electron Microscopy (SEM) and Transmission Electron Microscopy (TEM). The characterization results indicated that the prepared HAp and PBL-HAp were pure b-type carbonated HAp. The PBL-HAp was checked for its antibacterial activity using the well diffusion method and biofilm inhibitory activity by crystal violet assay against some common pathogens. The antibacterial activities against Staphylococcus aureus and biofilm inhibitory activities against Escherichia coli, Vibrio harveyi, Pseudomonas aeruginosa, and Staphylococcus aureus of Piper betel leaf extract coated HAp (PBL-HAp) were showed to be significant and offered a promising role for the development of potent dental biomaterials.

Absolute removal of ciprofloxacin and its degraded byproducts in aqueous solution using an efficient electrochemical oxidation process coupled with adsorption treatment technique.

Pharmaceutical-based contaminants are the major reasons for morbidity and mortality in aquatic animals and lead to several side effects and diseases in human community. Availability of proper, efficient, and cost-effective treatment technologies is still scarce. In this study, an efficient combined treatment technique (electrochemical oxidation and adsorption processes) was developed for the complete detoxification of most commonly used antibiotic, ciprofloxacin in aqueous solution. Electrochemical degradation of ciprofloxacin was performed using titanium-based tri-metal oxide mesh type anode, and the effective oxidative potential, electrolysis time, and pH for the degradation of ciprofloxacin were thoroughly evaluated. Sulfate, fluoride ions and toxic byproducts generated during electrochemical oxidation of ciprofloxacin were subsequently removed through a simple adsorption treatment using activated charcoal for 90 min. Further, the toxicity of the treated water was assessed with the nematode Caenorhabditis elegans species at different time intervals by observing the expressions of important stress-responsive genes viz., sod-3, hsp-16.2, ctl-1,2,3 and gst-4. The results exhibited that the combined process of electrochemical oxidation and adsorption treatment is simple, low-cost as well as effective to eliminate ciprofloxacin and its toxic byproducts in aqueous solution.