ABSTRACT
A radial segmental defect model of a rabbit was used to study the restoration effect on defects treated with chitosan-coated pressed calcium sulfate pellets combined with recombinant human bone morphogenetic protein-2 (rhBMP-2), coated pressed calcium sulfate pellets, and uncoated pressed calcium sulfate pellets. Nothing was implanted in the control group. After 4, 8, and 12 weeks, the results indicated that coated pressed calcium sulfate pellets combined with rhBMP-2 and coated pressed calcium sulfate pellets facilitated new bone formation on defected bones and that, particularly, the coated pressed calcium sulfate pellets combined with rhBMP-2 was more effective than the coated pressed calcium sulfate pellet. Histologic and tetracycline fluorimetric findings showed that the osteogenesis mechanism of chitosan-coated pressed calcium sulfate pellets is membrane bone formation, and the pellets showed slightly slower resorption that closely coincides with the growth rate of new bone.
Subject(s)
Bone Diseases/surgery , Bone Substitutes/therapeutic use , Calcium Sulfate/therapeutic use , Chitosan/therapeutic use , Coated Materials, Biocompatible/therapeutic use , Osteogenesis/drug effects , Absorbable Implants , Animals , Bone Density/drug effects , Bone Diseases/pathology , Bone Marrow/pathology , Bone Morphogenetic Protein 2 , Bone Morphogenetic Proteins/administration & dosage , Bone Morphogenetic Proteins/therapeutic use , Calcification, Physiologic/drug effects , Connective Tissue/pathology , Diaphyses/pathology , Diaphyses/surgery , Disease Models, Animal , Fluorescent Dyes , Fluorometry , Humans , Rabbits , Radius/pathology , Radius/surgery , Recombinant Proteins/administration & dosage , Recombinant Proteins/therapeutic use , Stress, Mechanical , Tetracycline , Time Factors , Transforming Growth Factor beta/administration & dosage , Transforming Growth Factor beta/therapeutic useABSTRACT
A chitosan-coated pressed calcium sulfate pellet combined with recombinant human bone morphogenetic protein 2 (rhBMP-2) has been developed with increased compressive strength and osteoinduction, but with a resorption profile only slightly slower than uncoated pellet. A radial segmental defect model of rabbit was used to study the restoration effect on defect treated with chitosan-coated pressed calcium sulfate pellet combined with rhBMP-2, coated pressed calcium sulfate pellet, and uncoated pressed calcium sulfate pellet. Nothing was implanted in the control group. After 4, 8, and 12 weeks, the results indicated that coated pressed calcium sulfate pellet combined with rhBMP-2 and coated pressed calcium sulfate pellet facilitated new bone formation on defected bone and that particularly the former was more effective than the latter.