ABSTRACT
Nanotherapeutics have been investigated for years, but only modest survival benefits were observed clinic. This is partially attributed to the short and rapid elimination of nanodrug after intravenous administration. In this study, a long circulation single wall carbon nanotube (SWCNT) complex was successfully fabricated through a new SWCNT dispersion agent, evans blue (EB). The complex was endowed with fluorescent imaging and photodynamic therapy ability by self-assembly loading an albumin coupled fluorescent photosensitizer, Chlorin e6 (Ce6) via the high affinity between EB and albumin. The yielding multifunctional albumin/Ce6 loaded EB/carbon nanotube-based delivery system, named ACEC, is capable of providing fluorescent and photoacoustic imaging of tumors for optimizing therapeutic time window. Synergistic photodynamic therapy (PDT) and photothermal therapy (PTT) were carried out as guided by imaging results at 24 h post-injection and achieved an efficient tumor ablation effect. Compared to PDT or PTT alone, the combined phototherapy managed to damage tumor and diminish tumor without recurrence. Overall, our study presents a SWCNT based theranostic system with great promising in dual modalities imaging guided PTT/PDT combined treatment of tumor. The applications of EB on SWCNT functionalization can be easily extended to the other nanomaterials for improving their in vivo stability and circulation time.
Subject(s)
Evans Blue , Microscopy, Fluorescence/methods , Nanotubes, Carbon , Neoplasms, Experimental/diagnostic imaging , Neoplasms, Experimental/drug therapy , Photochemotherapy/methods , Porphyrins/administration & dosage , Animals , Cell Line, Tumor , Chlorophyllides , Female , Mice , Mice, Inbred BALB C , Mice, Nude , Photoacoustic Techniques/methods , Photosensitizing Agents/administration & dosage , Porphyrins/chemistry , Theranostic Nanomedicine/methods , Treatment OutcomeABSTRACT
(19)F magnetic resonance imaging (MRI) is a powerful noninvasive, sensitive, and accurate molecular imaging technique for early diagnosis of diseases. The major challenge of (19)F MRI is signal attenuation caused by the reduced solubility of probes with increased number of fluorine atoms and the restriction of molecular mobility. Herein, we present a versatile one-pot strategy for the fabrication of a multifunctional nanoprobe with high (19)F loading (â¼2.0 × 10(8 19)F atoms per Cu1.75S nanoparticle). Due to the high (19)F loading and good molecular mobility that results from the small particle size (20.8 ± 2.0 nm) and ultrathin polymer coating, this nanoprobe demonstrates ultrahigh (19)F MRI signal. In vivo tests show that this multifunctional nanoprobe is suitable for (19)F MRI and photothermal therapy. This versatile fabrication strategy has also been readily extended to other single-particle nanoprobes for ablation and sensitive multimodal imaging.
Subject(s)
Copper/chemistry , Fluorine-19 Magnetic Resonance Imaging/methods , Mammary Neoplasms, Experimental/therapy , Nanoparticles/chemistry , Phototherapy/methods , Animals , Cell Survival/drug effects , Contrast Media/administration & dosage , Female , Fluorine Radioisotopes/administration & dosage , HeLa Cells , Humans , Injections, Subcutaneous , Mammary Glands, Animal , Mammary Neoplasms, Experimental/pathology , Mice , Mice, Inbred BALB C , Nanoparticles/administration & dosage , Nanoparticles/ultrastructure , Particle SizeABSTRACT
Uniform plasmonic Pd@Au core-shell bimetallic nanoplates are synthesized by seeded growth strategy. Surface modified with SH-PEG makes it good biocompatibility, prolonged blood circulation, and relatively high tumor accumulation. Enhanced tumor contrast effects can be obtained for in vivo photoacoustic/CT imaging after intravenous injection of Pd@Au-PEG. Moreover, efficient photothermal tumor ablation is achieved, guided by the imaging techniques. This work promises further exploration of the superiority of 2D nanostructures for in vivo biomedical applications.