ABSTRACT
Two experiments were conducted in this research. Experiment 1 investigated the spatial expression characteristics of calcium (Ca) and phosphorus (P) transporters in the duodenum, jejunum, and ileum of 21-day-old broilers provided with adequate nutrient feed. Experiment 2 evaluated the effects of dietary vitamin D3 (VD3) concentration (0, 125, 250, 500, 1,000, and 2,000 IU/kg) on growth performance, bone development, and gene expression levels of intestinal Ca and P transporters in 1-21-day-old broilers provided with the negative control diet without supplemental VD3. Results in experiment 1 showed that the mRNA levels of calcium-binding protein 28-kDa (CaBP-D28k), sodium-calcium exchanger 1 (NCX1), plasma membrane calcium ATPase 1b (PMCA1b), and IIb sodium-phosphate cotransporter (NaPi-IIb) were the highest in the broiler duodenum. By contrast, the mRNA levels of inorganic phosphate transporter 1 (PiT-1) and 2 (PiT-2) were the highest in the ileum. Results in experiment 2 showed that adding 125 IU/kg VD3 increased body weight gain (BWG), feed intake (FI), bone weight, and percentage and weight of Ca and P in the tibia and femur of 1-21-day-old broilers compared with the negative control diet (p < 0.05). The rise in dietary VD3 levels from 125 to 1,000 IU/kg further increased the BWG, FI, and weights of the bone, ash, Ca, and P (p < 0.05). No difference in growth rate and leg bone quality was noted in the broilers provided with 1,000 and 2,000 IU/kg VD3 (p > 0.05). Supplementation with 125-2,000 IU/kg VD3 increased the mRNA abundances of intestinal Ca and P transporters to varying degrees. The mRNA level of CaBP-D28k increased by 536, 1,161, and 28 folds in the duodenum, jejunum, and ileum, respectively, after adding 1,000 IU/kg VD3. The mRNA levels of other Ca and P transporters (PMCA1b, NCX1, NaPi-IIb, PiT-1, and PiT-2) increased by 0.57-1.74 folds by adding 1,000-2,000 IU/kg VD3. These data suggest that intestinal Ca and P transporters are mainly expressed in the duodenum of broilers. Moreover, the addition of VD3 stimulates the two mineral transporter transcription in broiler intestines.
ABSTRACT
The naringin extraction process was optimised using response surface methodology (RSM). A central component design was adopted, which included four parameters: extraction temperature (X1), material-liquid ratio (X2), extraction time (X3), and ultrasonic frequency (X4) of 74.79 °C, 1.58 h, 1:56.51 g/mL, and 28.05 KHz, respectively. Based on these optimal extraction conditions, naringin was tested to verify the model's accuracy. Naringin yield was 36.2502 mg/g, which was equivalent to the predicted yield of 36.0124 mg/g. DM101 macroporous adsorption resin was used to purify naringin. The effects of loading concentration, loading flow rate, and sample pH on the adsorption rate of naringin and the effect of ethanol concentration on the desorption rate of naringin were investigated. The optimum conditions for naringin purification using macroporous resins were determined. The optimal loading concentration, sample solution pH, and loading flow rate were 0.075 mg/mL, 3.5, and 1.5 mL/min, respectively. Three parallel tests were conducted under these conditions, and the average naringin yield was 77.5643%. Naringin's structure was identified using infrared spectroscopy and nuclear magnetic resonance. In vitro determination of the lipid-lowering activity of naringin was also conducted. These results showed that naringin has potential applications as a functional food for lowering blood lipid levels.
Subject(s)
Flavanones , Ultrasonics , Plant Extracts/chemistry , TemperatureABSTRACT
Iron is often found to be of excessive concentrations in laying hens' diets, which may cause antagonistic interactions with other minerals. This study was conducted to investigate how to supplement Zn and Mn in the diets without Fe supplementation. In experiment 1, 420 18-week Lohmann Brown layers were fed a basal diet or a basal diet supplemented with 30-0, 65-30 and 100-60 mg/kg of Zn and Mn, respectively. In experiment 2, 360 40-week Lohmann Brown layers were fed a basal diet or a basal diet supplemented with 15-0, 35-0 and 55-15 mg/kg of Mn and Zn, respectively. Minerals were supplemented in the form of sulfate. Egg production was improved by supplementing 30 mg/kg Zn or 65 mg/kg Zn in combination with 30 mg/kg Mn in experiment one. In experiment two, a significant reduction of egg performance occurred with 35 mg/kg Mn supplementation. Mn and/or Zn supplementation increased eggshell thickness in experiment one, and decreased yolk cholesterol in both experiments. Mn and/or Zn supplementation increased Zn and Mn excretion in both experiments. Serum growth hormone (GH), thyroxine (T(4) ), and insulin levels, or alkaline phosphatase (AP) activity were not affected by treatments; serum estrogen (E(2) ) and triiodothyronine (T(3) ) were different but there was no consistency by dietary treatments. This study demonstrates that 30 mg/kg supplemental Zn is necessary to obtain maximal egg production, and there seems to be no need to supply Mn in this type of diet.