ABSTRACT
The insecticidal activity is the result of a series of complex interactions between toxic substances as ligands and insect's enzymes as targets. Actually, synthetic insecticides used in pest control programs are harmful to the environment and may affect non-target organisms; thus, the use of natural products as pest control agents can be very attractive. In the present work, the toxic effect of aniseed (Pimpinella anisum L.) essential oil (EO) and its nanoemulsion (NE) against the red flour beetle Tribolium castaneum, has been evaluated. To assess the EO mode of action, the impact of sub-lethal concentrations of aniseed EO and NE was evaluated on enzymatic and macromolecular parameters of the beetles, including aspartate aminotransferase (AST), alanine aminotransferase (ALT), total protein, total lipids and glucose. Finally, a molecular docking study was conducted to predict the mode of action of the major EO and NE components namely E-anethole, Limonene, alpha-himalachalene, trans-Verbenol and Linalool at binding site of the enzymes AST and ALT. Herein, the binding location of the main compounds in both proteins are discussed suggesting the possible interactions between the considered enzymes and ligands. The obtained results open new horizons to understand the evolution and response of insect-plant compounds interactions and their effect predicted at the molecular levels and side effects of both animal and human.
Subject(s)
Monoterpenes/chemistry , Oils, Volatile/chemistry , Pimpinella/chemistry , Tribolium/drug effects , Alanine Transaminase/antagonists & inhibitors , Alanine Transaminase/chemistry , Animals , Aspartate Aminotransferases/antagonists & inhibitors , Aspartate Aminotransferases/chemistry , Emulsions/chemistry , Emulsions/pharmacology , Herb-Drug Interactions , Humans , Molecular Docking Simulation , Monoterpenes/pharmacology , Nanostructures/chemistry , Oils, Volatile/pharmacologyABSTRACT
The red flour beetle, Tribolium castaneum Herbst (Coleoptera: Tenebrionidae), is an economically important pest of stored products. As possible alternative to conventional insecticides for its management, plant essential oils have gained interest owing to their effectiveness and eco-friendly features. However, they also show some drawbacks, such as low stability, poor water solubility and diffusion, and limited persistence in the environment. A good strategy to overcome these disadvantages is represented by green nanotechnologies. Herein, we developed a nanoemulsion based on the essential oil from Pimpinella anisum L. (Apiaceae) containing 81.2% of (E)-anethole and evaluated its toxicity on T. castaneum adults and F1 progeny, as well as its morphological and histological impact. The aniseed oil nanoemulsion was characterized by the formation of a semi-solid interphase between oil and water; mean drop size was 198.9 nm, PDI was 0.303, zeta potential was - 25.4 ± 4.47 mV, and conductivity was 0.029 mS/cm. The nanoemulsion showed toxicity on T. castaneum (LC50 = 9.3% v/v), with a significant impact on its progeny. Morphological and histological damages triggered by feeding and exposure to the aniseed nanoemulsion were analyzed by scanning electron microscopy (SEM) and light microscopy. Overall, our findings showed that the development of nanoemulsions allows to improve the stability of P. anisum essential oil enhancing its efficacy against stored grain pests and contributing to reduce the use of harmful synthetic insecticides.