Chitosan-based nanosystems for cancer diagnosis and therapy: Stimuli-responsive, immune response, and clinical studies.

Cancer, a global health challenge of utmost severity, necessitates innovative approaches beyond conventional treatments (e.g., surgery, chemotherapy, and radiation therapy). Unfortunately, these approaches frequently fail to achieve comprehensive cancer control, characterized by inefficacy, non-specific drug distribution, and the emergence of adverse side effects. Nanoscale systems based on natural polymers like chitosan have garnered significant attention as promising platforms for cancer diagnosis and therapy owing to chitosan's inherent biocompatibility, biodegradability, nontoxicity, and ease of functionalization. Herein, recent advancements pertaining to the applications of chitosan nanoparticles in cancer imaging and drug/gene delivery are deliberated. The readers are introduced to conventional non-stimuli-responsive and stimuli-responsive chitosan-based nanoplatforms. External triggers like light, heat, and ultrasound and internal stimuli such as pH and redox gradients are highlighted. The utilization of chitosan nanomaterials as contrast agents or scaffolds for multimodal imaging techniques e.g., magnetic resonance, fluorescence, and nuclear imaging is represented. Key applications in targeted chemotherapy, combination therapy, photothermal therapy, and nucleic acid delivery using chitosan nanoformulations are explored for cancer treatment. The immunomodulatory effects of chitosan and its role in impacting the tumor microenvironment are analyzed. Finally, challenges, prospects, and future outlooks regarding the use of chitosan-based nanosystems are discussed.

ZnO/black phosphorus/C3N4 composite: An effective photocatalyst for Cr (VI) reduction and degradation of rhodamine B.

The utilization of photocatalysts offers a promising approach for the removal of Cr (VI) and rhodamine dyes. Through the generation of reactive species and subsequent degradation reactions, photocatalysis provides an efficient and environmentally friendly method for the remediation of wastewater. In this study, we have synthesized an n-p-n heterojunction of carbon nitride (C3N4), zinc oxide (ZnO), and black phosphorus (BP) through the sonication-stirring method. The photocatalytic ability of this composite was examined for the decomposition rhodamine B (RhB) and detoxification of hexavalent chromium ion (up to 97% during 80 min) under Xenon irradiation. The results of trapper experiments indicated that the active species were hydroxyl radical (˙OH), electron (e-), and superoxide anion radical (˙O2-). Based on the obtained potential of the lowest unoccupied molecular orbitals (LUMO) and the highest occupied molecular orbital (HOMO) for the mentioned semiconductors, through Mutt-Schottky results, the double Z-scheme mechanism was proposed for the studied process. The electrochemical impedance spectroscopy data exhibited good charge transfer for the evaluated composite versus the pure compounds. The impressive separation of holes and electrons along with the low recombination were confirmed by the responses of photocurrent and quenching the photoluminescence (pl) intensity for the composite, respectively. The current density of the composite recorded 66.6%, 87.3%, and 92% higher than those of BP, C3N4, and ZnO, indicating an excellent electron-hole separation for the ternary composite compared to the pure semiconductors. Diffuse reflectance spectra (DRS) data revealed 2.9, 3.17, 1.15, and 2.63 eV as the band gap values for C3N4, ZnO, BP, and composite. The rate constant of the new composite to remove RhB and reduce hexavalent chromium were about 4.79 and 2.64 times higher than that of C3N4, respectively.

Eco-friendly synthesis of carbon nanotubes and their cancer theranostic applications.

Carbon nanotubes (CNTs) with attractive physicochemical characteristics such as high surface area, mechanical strength, functionality, and electrical/thermal conductivity have been widely studied in different fields of science. However, the preparation of these nanostructures on a large scale is either expensive or sometimes ecologically unfriendly. In this context, plenty of studies have been conducted to discover innovative methods to fabricate CNTs in an eco-friendly and inexpensive manner. CNTs have been synthesized using various natural hydrocarbon precursors, including plant extracts (e.g., tea-tree extract), essential oils (e.g., eucalyptus and sunflower oil), biodiesel, milk, honey, and eggs, among others. Additionally, agricultural bio-wastes have been widely studied for synthesizing CNTs. Researchers should embrace the usage of natural and renewable precursors as well as greener methods to produce various types of CNTs in large quantities with the advantages of cost-effectiveness and environmentally benign features. In addition, multifunctionalized CNTs with improved biocompatibility and targeting features are promising candidates for cancer theranostic applications owing to their attractive optical, chemical, thermal, and electrical properties. This perspective discusses the recent developments in eco-friendly synthesis of CNTs using green chemistry-based techniques, natural renewable resources, and sustainable catalysts, with emphasis on important challenges and future perspectives and highlighting techniques for the functionalization or modification of CNTs. Significant and promising cancer theranostic applications as well as their biocompatibility and cytotoxicity issues are also discussed.

MXenes in photomedicine: advances and prospects.

MXenes and their related nanocomposites with superior physicochemical properties such as high surface area, ease of synthesis and functionalization, high drug loading capacity, collective therapy potentials, pH-triggered drug release behavior, high photothermal conversion, and excellent photodynamic efficiency have been explored as alluring materials in photomedicine; the application of photons in medicine is facilitated for imaging and various disease treatment methods such as photothermal cancer/tumor ablation. Non-invasive theranostic strategies with synergistic activities have been developed using photothermal, photodynamic, and magnetic therapies together with remotely controlled drug/gene delivery for the diagnosis and treatment of various malignant diseases. Photothermal/photodynamic therapy and photoacoustic imaging using MXene-based structures have shown great promise in cancer phototherapy. However, hybridization and surface functionalization should be further explored to obtain biocompatible MXene-based composites/platforms with unique properties, high stability, and improved functionality in photomedicine. Toxicological and long-term biosafety assessments as well as clinical translation evaluations ought to be given high priority in research. Although some limited studies have revealed the excellent potentials of MXenes and their derivatives in photomedicine, further steps should be taken towards extensive research and detailed analysis in the field of optimizing the properties and improving the performance of these materials with a clinical and industrial outlook. Optical biosensing platforms have been developed along with electrochemical sensors and wearable sensors constructed from MXenes and their derivatives; future studies warrant the comprehensive analysis of optical transduction aspects such as colorimetry, electrochemiluminescence, photoluminescence, surface-enhanced Raman scattering, and surface plasmon resonance. Herein, the potentials of MXenes in photomedicine are deliberated encompassing important challenges and future research directions.

Biosynthesis of Zn-doped CuFe2O4 nanoparticles and their cytotoxic activity.

Zn-doped CuFe2O4 nanoparticles (NPs) were eco-friendly synthesized using plant extract. These nanoparticles were characterized by X-ray diffraction, Fourier-transform infrared spectroscopy, scanning electron microscope (SEM), energy-dispersive X-ray spectroscopy and thermal gravimetric analysis (TGA). SEM image showed spherical NPs with size range less than 30 nm. In the EDS diagram, the elements of zinc, copper, iron, and oxygen are shown. The cytotoxicity and anticancer properties of Zn-doped CuFe2O4 NPs were evaluated on macrophage normal cells and A549 lung cancer cells. The cytotoxic effects of Zn-doped CuFe2O4 and CuFe2O4 NPs on A549 cancer cell lines were analyzed. The Zn-doped CuFe2O4 and CuFe2O4 NPs demonstrated IC50 values 95.8 and 278.4 µg/mL on A549 cancer cell, respectively. Additionally, Zn-doped CuFe2O4 and CuFe2O4 NPs had IC80 values of 8.31 and 16.1 µg/mL on A549 cancer cell, respectively. Notably, doping Zn on CuFe2O4 NPs displayed better cytotoxic effects on A549 cancer cells compared with the CuFe2O4 NPs alone. Also spinel nanocrystals of Zn-doped CuFe2O4 (~ 13 nm) had a minimum toxicity (CC50 = 136.6 µg/mL) on macrophages J774 Cell Line.

Quantum dots against SARS-CoV-2: diagnostic and therapeutic potentials.

The application of quantum dots (QDs) for detecting and treating various types of coronaviruses is very promising, as their low toxicity and high surface performance make them superior among other nanomaterials; in conjugation with fluorescent probes they are promising semiconductor nanomaterials for the detection of various cellular processes and viral infections. In view of the successful results for inhibiting SARS-CoV-2, functional QDs could serve eminent role in the growth of safe nanotherapy for the cure of viral infections in the near future; their large surface areas help bind numerous molecules post-synthetically. Functionalized QDs with high functionality, targeted selectivity, stability and less cytotoxicity can be employed for highly sensitive co-delivery and imaging/diagnosis. Besides, due to the importance of safety and toxicity issues, QDs prepared from plant sources (e.g. curcumin) are much more attractive, as they provide good biocompatibility and low toxicity. In this review, the recent developments pertaining to the diagnostic and inhibitory potentials of QDs against SARS-CoV-2 are deliberated including important challenges and future outlooks. © 2022 Society of Chemical Industry (SCI).

Green synthesis of bimetallic ZnO-CuO nanoparticles and their cytotoxicity properties.

In this study, a simple and green strategy was reported to prepare bimetallic nanoparticles (NPs) by the combination of zinc oxide (ZnO) and copper oxide (CuO) using Sambucus nigra L. extract. The physicochemical properties of these NPs such as crystal structure, size, and morphology were studied by X-ray diffraction (XRD), field emission gun scanning electron microscopy (FEG-SEM), and transmission electron microscopy (TEM). The results suggested that these NPs contained polygonal ZnO NPs with hexagonal phase and spherical CuO NPs with monoclinic phase. The anticancer activity of the prepared bimetallic NPs was evaluated against lung and human melanoma cell lines based on MTT assay. As a result, the bimetallic ZnO/CuO NPs exhibited high toxicity on melanoma cancer cells while their toxicity on lung cancer cells was low.

Leishmanicidal activities of biosynthesized BaCO3 (witherite) nanoparticles and their biocompatibility with macrophages.

The aim of this study was cost-effective and greener synthesis of barium carbonate (BaCO3 or witherite) nanoparticles with economic importance, and to evaluate their therapeutic potentials and biocompatibility with immune cells. Barium carbonate nanoparticles were biosynthesized using black elderberry extract in one step with non-toxic precursors and simple laboratory conditions; their morphologies and specific structures were analyzed using field emission scanning electron microscopy with energy dispersive X-ray spectroscopy (FESEM-EDX). The therapeutic capabilities of these nanoparticles on the immune cells of murine macrophages J774 and promastigotes Leishmania tropica were evaluated. BaCO3 nanoparticles with IC50 = 46.6 µg/mL were more effective than negative control and glucantium (positive control) in reducing promastigotes (P < 0.01). Additionally, these nanoparticles with a high value of cytotoxicity concentration 50% (CC50) were less toxic to macrophage cells than glucantime; however, they were significantly different at high concentrations compared to the negative control.

MXenes for Cancer Therapy and Diagnosis: Recent Advances and Current Challenges.

MXenes endowed with several attractive physicochemical attributes, namely, specific large surface area, significant electrical conductivity, magnetism, low toxicity, luminescence, and high biocompatibility, have been considered as promising candidates for cancer therapy and theranostics. These two-dimensional (2D) nanostructures endowed with photothermal, chemotherapeutic synergistic, and photodynamic effects have shown promising potential for decidedly effectual and noninvasive anticancer treatments. They have been explored for photothermal/chemo-photothermal therapy (PTT) and for targeted anticancer drug delivery. Remarkably, MXenes with their unique optical properties have been employed for bioimaging and biosensing, and their excellent light-to-heat transition competence renders them an ideal biocompatible and decidedly proficient nanoscaled agent for PTT appliances. However, several important challenging issues still linger regarding their stability in physiological environments, sustained/controlled release of drugs, and biodegradability that need to be addressed. This Perspective emphasizes the latest advancements of MXenes and MXene-based materials in the domain of targeted cancer therapy/diagnosis, with a focus on the current trends, important challenges, and future perspectives.

Starch, cellulose, pectin, gum, alginate, chitin and chitosan derived (nano)materials for sustainable water treatment: A review.

Natural biopolymers, polymeric organic molecules produced by living organisms and/or renewable resources, are considered greener, sustainable, and eco-friendly materials. Natural polysaccharides comprising cellulose, chitin/chitosan, starch, gum, alginate, and pectin are sustainable materials owing to their outstanding structural features, abundant availability, and nontoxicity, ease of modification, biocompatibility, and promissing potentials. Plentiful polysaccharides have been utilized for making assorted (nano)catalysts in recent years; fabrication of polysaccharides-supported metal/metal oxide (nano)materials is one of the effective strategies in nanotechnology. Water is one of the world's foremost environmental stress concerns. Nanomaterial-adorned polysaccharides-based entities have functioned as novel and more efficient (nano)catalysts or sorbents in eliminating an array of aqueous pollutants and contaminants, including ionic metals and organic/inorganic pollutants from wastewater. This review encompasses recent advancements, trends and challenges for natural biopolymers assembled from renewable resources for exploitation in the production of starch, cellulose, pectin, gum, alginate, chitin and chitosan-derived (nano)materials.

Green biosynthesis of silver nanoparticles using Althaea officinalis radix hydroalcoholic extract.

The objectives were to study the potential of Althaea officinalis radix in production of silver NPs, and the effect of the extract ethanol concentration on the produced NPs. Seventy and ninety-six percent hydroalcoholic extracts were prepared by percolation of the plant powder. The extract was concentrated by rotary evaporator and then freeze-dried. Silver ions were determined using atomic absorption analysis. The NPs were characterized by Nano-Zeta Sizer and TEM. Both of 70% and 96% of hydroalcoholic extracts of A. officinalis radix successfully synthesized spherical and poly-dispersed silver NPs. The conversion was fast and almost completed in 5 h.

Synthesis of silver nanoparticles using methanol and dichloromethane extracts of Pulicaria gnaphalodes (Vent.) Boiss. aerial parts.

The objectives were to study the potential of Pulicaria gnaphalodes (Vent.) Boiss. aerial parts in production of nanoparticles and the effect of the extraction solvent on the produced nanoparticles. Methanol and dichloromethane extracts were prepared by percolation of the plant powder. Both the extracts of P. gnaphalodes (Vent.) Boiss. successfully produced small and polydispersed nanoparticles with low aggregates in early hours of the biotransformation. Methanol extract produced spherical and many single nanoparticles, whereas dichloromethane produced porous polyhedral and more aggregated nanoparticles. Methanol extract of this plant seems to be quiet useful for industrial scale production of nanoparticles.

Green biosynthesis of silver nanoparticles using Quercus brantii (oak) leaves hydroalcoholic extract.

CONTEXT: There is an ever-growing need to develop green, non-toxic, and eco-friendly procedures for synthesis and assembly of nanoparticles (NPs) with the desired morphologies and sizes. The hydroalcoholic extract of Persian oak leaves [Quercus brantii Lindl. (Fagaceae)] contains high content of phenolic and flavonoid compounds with strong antioxidant activities, and it seems that this plant can be considered a good candidate for metal nanoparticle synthesis. OBJECTIVE: The potential of Q. brantii leaves in the production of silver NPs and the effect of the extract ethanol concentration on the produced NPs were studied. MATERIALS AND METHODS: Quercus brantii leaves were freshly collected, air-dried at room temperature, powdered, and sieved. Hydroalcoholic extracts (70% and 96%) were prepared by percolation of the plant powder. The reaction mixtures contained (final concentrations): AgNO3 (1 mM) as the substrate, plant extract as the biocatalyst, and phosphate buffer (pH = 7, 100 mM) as the reaction medium. Silver ions were determined using atomic absorption analysis. Particle size distribution of NPs was analyzed using Nano-Zeta Sizer (Malvern Instruments Ltd, Malvern, UK). Samples for TEM were prepared by drop-coating the silver nanoparticle suspensions onto carbon-coated copper grids. RESULTS: Hydroalcoholic extract (96%) of Q. brantii successfully produced quite small (as small as 0.83 nm and the mean size of 6 nm), spherical, and poly-dispersed NPs with low aggregates. The conversion was fast and completed in 5 h. DISCUSSION AND CONCLUSION: This plant and the extraction method seem to be quiet attractive for industrial scale production of NPs.

Green synthesis of silver nanoparticles using Pinus eldarica bark extract.

Recently, development of reliable experimental protocols for synthesis of metal nanoparticles with desired morphologies and sizes has become a major focus of researchers. Green synthesis of metal nanoparticles using organisms has emerged as a nontoxic and ecofriendly method for synthesis of metal nanoparticles. The objectives of this study were production of silver nanoparticles using Pinus eldarica bark extract and optimization of the biosynthesis process. The effects of quantity of extract, substrate concentration, temperature, and pH on the formation of silver nanoparticles are studied. TEM images showed that biosynthesized silver nanoparticles (approximately in the range of 10-40 nm) were predominantly spherical in shape. The preparation of nano-structured silver particles using P. eldarica bark extract provides an environmentally friendly option, as compared to currently available chemical and/or physical methods.