Synergistic Influence of Yeast Extract and Calcium Oxide Nanoparticles on the Synthesis of Bioactive Antioxidants and Metabolites in Swertia chirata In Vitro Callus Cultures.

The challenges in the production of metabolites of medicinal potential from wild plants include low yields, slow growth rates, seasonal variations, genetic variability and regulatory as well as ethical constraints. Overcoming these challenges is of paramount significance and interdisciplinary approaches and innovative strategies are prevalently applied to optimize phytoconstituents' production, enhance yield, biomass, ensure sustainable consistency and scalability. In this study, we investigated the effects of elicitation with yeast extract and calcium oxide nanoparticles (CaONPs) on in vitro cultures of Swertia chirata (Roxb. ex Fleming) Karsten. Specifically, we examined the effects of different concentrations of CaONPs in combination with different concentrations of yeast extract on various parameters related to callus growth, antioxidant activity, biomass and phytochemical contents. Our results showed that elicitation with yeast extract and CaONPs had significant effects on the growth and characteristics of callus cultures of S. chirata. The treatments involving yeast extract and CaONPs were found to be the most effective in increasing the contents of total flavonoid contents (TFC), total phenolic contents (TPC), amarogentin and mangiferin. These treatments also led to an improvement in the contents of total anthocyanin and alpha tocopherols. Additionally, the DPPH scavenging activity was significantly increased in the treated samples. Furthermore, the treatments involving elicitation with yeast extract and CaONPs also led to significant improvements in callus growth and characteristics. These treatments promoted callus response from an average to an excellent level and improved the color and nature of the callus from yellow to yellow-brown and greenish and from fragile to compact, respectively. The best response was observed in treatments involving 0.20 g/L yeast extract and 90 ug/L CaONPs. Overall, our findings suggest that elicitation with yeast extract and CaONPs can be a useful strategy for promoting the growth, biomass, phytochemical contents and antioxidant activity of callus cultures of S. chirata in comparison to wild plant herbal drug samples.

Green Synthesis of Zinc Oxide Nanoparticles Using Viscum album Extracts: Unveiling Bioactive Compounds, Antibacterial Potential, and Antioxidant Activities.

The current study explored the antioxidant and antibacterial capabilities of zinc oxide nanoparticles (ZnONPs) synthetized using methanolic leaf extracts of the medicinal herb Viscum album. Through TEM investigation and UV-Vis analysis, which peaked at 406 nm, the synthesis of ZnONPs was verified. TEM analyses showed that the synthesized ZnONPs had a size distribution with an average of 13.5 nm and a quasi-spherical shape. Forty-four phytoconstituents were found in the methanolic leaf extracts of V. album. Additionally, a comparison of the antibacterial effectiveness and antioxidant capacity of aqueous and methanolic extracts of wild-grown V. album phytomedicine and green-manufactured ZnONPs was conducted. The green-generated ZnONPs were examined against Escherichia coli, Staphylococcus aureus, and Pseudomonas aeruginosa and shown to have superior antibacterial activity by 22%, 66%, and 44%, respectively, as compared to wild herbal medicinal extracts. Since the ZnONPs' aqueous extracts had higher concentrations of DNA gyrase-B inhibitory components, they were shown to be more effective in limiting bacterial growth. In contrast to the percentages of 49% and 57% for a wild plant extract, the aqueous- and methanolic-extract-mediated green ZnONPs, with a 100 g/mL concentration, showed 94% and 98% scavenging capacity for DPPH free radicals, respectively. However, methanolic extracts were more effective than aqueous extracts in terms of the antioxidant analyses. This study establishes that greenly produced ZnONPs have the potential to be used in nanomedicine to treat bacteria that are resistant to a variety of drugs, as well as those with reactive oxygen species toxicity.

Analysis of antidiabetic, antiulcer and analgesic potential of traditional ethnomedicinal plant Emex spinosa (L.) Campd. from Azad Jammu and Kashmir.

In this research antidiabetic, analgesic and antiulcer potential of traditional ethnomedicinal plant: Emex spinosa (L.) Campd. (Family Polygonaceae) was evaluated by extracting its phytoconstituents using methanol (MeOH) solvent through maceration protocol. The quantitative phytochemical analysis of the extract revealed flavonoids were highest in leaf extract (15.63±0.93 mg/mL) and with (11.5±0.57 mg/mL) in stem. Alkaloids and tanins were also present in the samples in various conc. while saponins were absent. To confirm pharmaceutical potential of plant against ulcer, diabetes and analgesic infirmities, a model experimental animal wistar albino rats (Rattus norvegicus) were used. In antiulcer study, using hot plate method the maximum results were observed with 250 mg/kg in the 2.5 hours of study. The leaf extract showed a 40.41±2.73 latency time and the fruit with a 36.77±2.41, and the stem with a 27.85±3.09, which was comparable to the standard drug Aspirin, i.e., 47.5±0.57. For analysis of antiulcer potential of the plants parts doses of 250 and 500 mg/kg was applied to check the reclamation of ethanol-induced acute ulcer and of Aspirin-induced chronic ulcer of stomach. In order to confirm efficacy of the drug potential of plant following parameters like microscopic evaluation, gastric volume, total acidity, mucosa weight, ulcer index, pH and histopathology of stomach were analyzed. In antidiabetic analysis, in an acute study after a single dose of 500 mg/kg extract after 2hrs the blood glucose levels were 367±51.09958NS, 416±59.79548NS, 437.5±61.96437NS mg/dL for leaf, stem and fruit, respectively. After4hrs 351.75±88.27644NS mg/dl, 448.25±25.64948NS mg/dl, 445.25±27.07205NS mg/dl and after 6hrs 354.5±92.70428NS, 442±24.60691NS, a440±33.16625NS mg/dl, respectively. The analgesic activity was explored by applying hot plate, tail flick and formalin paw licking method. In hot plate method the maximum results were observed with 250mg/kg in the 2.5 hours of study. The leaf extract showed a 40.41±2.73 latency time and the fruit with a 36.77±2.41 and the stem with a 27.85±3.09, which was comparable to the standard drug Aspirin, i.e., 47.5±0.57. The respective plant extracts at 250mg/kg showed a gradual rise in latency time when compared to the control. It was concluded that all three components of E. spinosa perform proved to be significant as potential source of herbal medicines to cure different prevalently occurring diseases. Furthermore, it was confirmed through results analysis that plant t can be used to discover novel drug using dedicated high throughput techniques and ethnopharmacological approaches.

Ethnobotanical inventory and medicinal perspectives of herbal flora of Shiwalik mountainous range of District Bhimber, Azad Jammu and Kashmir, Pakistan.

The present study was carried out to document traditional ethnobotanical knowledge (TEK) of herbal flora of District Bhimber, Azad Jammu and Kashmir (AJK), Pakistan to explore medicinal potential of wild indigenous plants (WIPs) for drug discovery. The research data was conducted during years 2015-2017 using questionnaire proforma employing structured and semi-structured interview models. The informants belonged from indigenous communities comprising of both genders with age ranging from 35-105 years. The interviews were conducted using local translator or guide who knew the dialects of all local languages. The study produced ethnobotanical inventory of 173 herbal species belonging to 45 families and out of these Poaceae was dominant family with 27 species. It was explored that maximum herbal species depicted multi-usage especially food, fodder and fuel. Among 173 herbal plant species, 69% species were used as fodder, 72% species as fuel source, 9.8% as ethnoveterinary medicines, 16% for home construction, 12% for cosmetics, 5.2% as honeybee plants and 2.7% were used as fiber source by the local people. Many local plants have been in promulgation for cure of different diseases in traditional cultures such as for cure of stomach problems, cough, cancer, jaundice, kidney diseases, diabetes, snake biting and tooth problems. Different parts of plants such as leaf, root, stem bark, flower, seed and gums are used for the treatment of different diseases by the local people. The major aliments being cured are classified into 12 disease categories by using informant consensus factor (ICF) protocol. According to ICF, the highest numbers of plant species were used against wound healing, snake bite, skin diseases, eye diseases and asthma. Fidelity level (FL) was assessed to check the reliability and use consistency of herbal drugs by the indigenous communities of the study area. The use value index (UVI) of different herb species ranged from 0.29 to 0.57 while the highest value was calculated for Alternanthera pungens L. (UVI: 0.57). Relative frequency of citation (RFC) value was calculated on the bases of the response of the interviewees recorded during survey in correlation with authenticating of traditional data. The RFC values represented the relative popularity of individual species in study area according to their use values. The highest value was calculated for Alternanthera pungens L. (0.90) and followed by Achyranthes aspera L. (0.80. The study reveals that many species are known for commonly used in traditional ethnomedicines (TEMs). Due to different biotic and abiotic factors in conjunction with climatic changes many herbal flora of Shiwalik mountain range (SMR) of District Bhimber of AJK is under threat. The factors like habitat loss, overgrazing, construction of communication infrastructure, silviculture practices, shelter construction (houses) and other more domestic use of wild land by clearing wild lands are boosting towards plant biodiversity loss. There is dare need to work on comprehensive exploration of TEMs to discover neo drugs from wild indigenous plants and do work for conservation of wild flora of the area for future generations.

Ethnobotanical and conservation studies of tree flora of Shiwalik mountainous range of District Bhimber Azad Jammu and Kashmir, Pakistan.

The present study was carried out to explore and document traditional ethnobotanical knowledge of indigenous rural communities of Shiwalik mountainous range regarding tree flora of the area, District Bhimber of Azad Jammu and Kashmir, Pakistan. The local people of the area primarily depend on wild flora for life sustenance and cure of different infirmities. In this research, data was collected through visual appraisal and participatory rural approach using questionnaire method by applying semi-structured and structured-interview protocols (S4 Table). To validate and explore novelty of research work, various quantitative ethnobotanical indices like informant consensus factor, use value index, fidelity level, relative frequency of citation, relative importance of plants, rank order of priority, Jaccard index and priority ranking were used. The highest ICF was found for jaundice with 0.91 value followed by ICF values of 0.89, 0.86 and 0.85 for wounds, skin diseases and stomach pains, respectively. Fidelity level predicted that Azadirachta indica (Indian lilac tree) ranked first (100%) for its prevalent use in ethnomedicines. Highest use value index (0.61) was found for Azadirachta indica while the highest relative frequency of citation (55) was measured for Melia azedarach. The relative order of priority index was the highest for Azadirachta indica and Acacia arabica L. (ROP = 100) depicting their prevalent use in ethnomedicines. For EB study, the highest relative importance (50.8) was found for Azadirachta indica with good number of agro-uses and its seeds and bark are sold or marketed in local markets to prepare herbal therapeutics by indigenous practioners. The Jaccard index depicted that many traditional ethnomedicinal uses of prescribed trees were novel in recipe form or first time reported such as Senegalia modesta (Phulai) resin was used to prepare 'Panjoori' a local tonic prepared from cow or buffalo butter mixing with seeds of Papaver somnifera (Kashkhash) and Prunus amygdalus (Badaam). The wood of many trees like Pinus roxburghii (pine), Dalbergia sissoo (rosewood), Senegalia modesta (Phulai), Acacia. Arabica (kikar), Bombax cieba (cotton tree) is commercially sold as timber in markets (S3 Table). The research proved that population explosion and climate changes have triggered severe biotic and abiotic pressure on tree flora of the study area of Bhimber, AJK. The research describes that plants like Terminalia belerica (belerica), Terminalia arjuna (arjun tree), Cassia fistula (Indian laburnum), Butea monosperma (bastard teak), Phyllanthus emblica (Indian gooseberry), Morus laevigata (Shah toot), Bauhinia variegata (orchid tree) and Flacourtia indica (Indian plum/ kakoo) are threatened species and their population is highly reduced and if reclamation measures for their conservation are not taken, it may lead towards their complete loss from the area. This research recommends sustainable ethnobotanical use of tree flora, their growth and conservation for green and ecofriendly environment for safe and secure future of human generation.

Integrated phosphorus nutrient sources improve wheat yield and phosphorus use efficiency under sub humid conditions.

Majority of Pakistani soils are deficient in phosphorus. Phosphorus is usually applied in form of synthetic fertilizer. However integrated use of P from synthetic and organic sources can be more profitable and beneficial on sustainable basis. Field trials were conducted at research farm University of Poonch, Rawalakot, AJK, Pakistan for two consecutive years to check the comparative effects of synthetic fertilizer and organic manures applied alone and in combinations on the phosphorus use efficiency (PUE), wheat yield and yield components. Shafaq-06 cultivar of wheat was used as test cultivar. Ten treatments were included: (I) Control (P0) without application of fertilizer; (II) SSP @ 60 kg/ha (P60SSP); (III) SSP @ 90 kg/ha (P90SSP); (IV) SSP @ 120 kg/ha (P120SSP); (V) PM @60 kg/ha (P60PM); (VI) PM @90 kg/ha (P90PM); (VII) PM @120 kg/ha (P120PM); (VIII) SSP @30 kg/ha + PM @30 kg/ha (P30SSP+30PM); (IX) SSP @45 kg/ha + PM @45 kg/ha (P45SSP+45PM); (X) SSP @60 kg/ha + PM @60 kg/ha (P60SSP+60PM) which were laid out under the Randomized Complete Block Design. Significantly higher values for yield of grain (2397 kg/ha) was recorded with PM + SSP @ 60 kg P2O5 ha-1 each. Likewise, FPUE, PIR of wheat and AFPUE was quite higher with combined use of PM and SSP i.e. P60SSP+60PM treatment. Additionally, increase in PUE, wheat yield and yield components associated with combined treated plot would help to minimize the use of high cost synthetic mineral fertilizers and represents an environmentally and agronomically sound management strategy.

Inventorization of traditional ethnobotanical uses of wild plants of Dawarian and Ratti Gali areas of District Neelum, Azad Jammu and Kashmir Pakistan.

BACKGROUND: An ethnobotanical expedition was conducted to document the traditional ethnobotanical (TEB) uses of wild flora of Dawarian and Ratti Gali villages of District Neelam, Azad Jammu and Kashmir (AJK) Pakistan. District Neelam has rich plant diversity and is hub of many endemic plant species while the study areas are not yet explored. The research area: Dawarian and Rati Gali (DRG) area is mountaineous terrain and villages are located on far and farther distances. DRG area has rich biocultural and plant diversity comprising of different ethnic groups of Kashmir state. The current research was aimed to explore and document traditional medicines (TEMs) and other domestic and commercial uses of wild plants. This study will assist to evaluate conservation and commercial worth of wild flora which can be potential candidate for drug discovery through ethnopharmacological analysis. METHODS: The current quantitative ethnobotanical research was carried out in 2018 by interviewing 150 indigenous informants (90 male and 60 female) of DRG area using questionnaire applying structured and semi structured interview methodology. Data analysis was analyzed by using quantitative ethnobotanical statistical tools such as fidelity level (FL), informant consensus factor (ICF), Spearman's rank correlation (SRC) and data matrix ranking (DMR). RESULTS: The indigenous people of DRG area use wild plants in their daily life to cope life necessities i.e. food, vegetables, fodder, fuel, shelter, timber and herbal medicines. TEMs are primarily used to cure different infirmities like diabetics, asthma, dysentery, constipation, cold, fever, joint pain, wound healing, cancer, cardiovascular disorders, epilepsy, kidney infections and many types of skin diseases. Current study revealed the data of 103 wild plants species belonging to 46 plant families from selected areas of District Neelum, AJK. Results depicted that Asteraceae ranked 1st (12 plants spp). Among plant parts used leaf ranked 1st (18%), followed by seed (17%) and root (13%). While prevalent form recipe mode was decoction (20%), followed by powder (17%) and extract (14%) and fodder was highest (37%) EB use-form fodder, followed by food (32%) and fuel (17%). Quantitative ethnobotanical analysis (QEA) was carried to find the reliability and novelty of the study. Five plant species including Berberis lyceum (FL = 97.78%), Isodon rugosus (FL = 95.71%), Saussurea lappa (FL = 94.74%), Aconitum heterophyllum (FL = 92.71%) and Taxus baccata (91.58%) had shown high fidelity level which confirmed that these plants have high medicinal worth in study area. The highest value (0.94) of ICF was for diseases group "tuberculosis and leucorrhea", followed by stomachache and flatulence (0.93), diabetics and blood pressure (0.92) and asthma and chest infections (0.88). For other uses fuel with ICF (0.83) ranked first and second was hedging and thatching (ICF = 0.82) where people use plants or their parts for construction. Spearman's rank correlation (SRC) test indicated that number of TEB uses increases if number of species is increased. Jaccard index (JI) analysis depicted that 56.31% plants are being used as TEMs which are first time explored from the study area. While 26.21% plants are being used in different TEB uses which are different from past cited literature. These novel findings of research indicate that wild flora of the study area has great potential for novel drug discovery and provision of materialist services for the indigenous communities. CONCLUSION: The present research revealed that TEMs uses of 58 plants are novel being first time reported from the study area (DRG) of District Neelam of AJK. The results showed that plants like Acer cappadocicum, Ajuga bracteosa and Swertia paniculata are used to cure diabetes, Viscum album, Viola canescens, Taxus baccata are used for cure of cancer, Isodon rugosus, Polygala chinensis are used in TEMs for treating cardiovascular disorders and Anaphalis triplinervis is used for epilepsy. Berberis lyceum, Ajuga bracteosa, Aconitum heterophyllum, Bistorta amplexicaule, Saussurea lapa and Jurinea dolomiaea are severely threatened and there is urgent need to do conservation measures for available of valuable MPs to the indigenous communities for life necessities and for future research. The current study will also be useful addition in ethnobotanical database, preservation of traditional culture and drug discovery and drug development through future ethnopharmacological research.

Ethnomedicinal and folklore inventory of wild plants used by rural communities of valley Samahni, District Bhimber Azad Jammu and Kashmir, Pakistan.

The current study describes ethnobotanical (EB) and traditional ethnomedicinal (TEMs) uses of medicinal plants (MPs) of Tehsil Samahni area of Azad Jammu and Kashmir, Pakistan. Indigenous inhabitants of the area mostly reside in remote mountainous areas and depend on wild plants for life necessities and treatment of different diseases. This paper presents first quantitative ethnobotanical study describing the popularity level of MPs in indigenous communities by using relative popularity level (RPL) and rank order of priority (ROP) indices. The data of TEMs and EB uses were collected from 200 interviewees consisting of traditional herbal practioners (110) and farmers (90) from study area using structured and semi-structured interview methodology during year 2019. It was found that illiterate peasants have more knowledge and practice of TEMs than other people. A total of 150 plant species belonging to 58 families with botanical and local names, parts used, mode of recipes preparation, administration mechanism and TEMs uses were documented in systematic manner. It was explored that highest number (7.69%) of plants of Moraceae were used in TEMs and followed by species of Asteraceae (6.29%). The ethnobotanical data was analyzed by using quantitative ethnobotanical tools viz: informant consensus factor (ICF), fidelity level (FL), relative frequency of citation (RFC), use value index (UVI), relative importance of plants (RIP), relative populatiry level (RPL) and rank order of popularity (ROP). The leaf ranked first (36.49%) and fruit 2nd (12.07%) being used in TEMs while prevalent use-form was decoction (29.28%), followed by tea (23.05%) and preferably taken as oral (46.66%). ICF analysis proved that diabetes, hypertension, rabies, snake sting and joint pains were the most prevalent occurring infirmities. The highest RFC (0.95) was found for Acacia modesta, followed by Boerhavia procumbens (RFC:0.87) and Berberis lycium (RFC:0.85). The relative importance of MPs was calculated by using UVI and Moringa oleifera showed highest (1.38), followed by Zanthoxylum armatum (1.25) and Withania somnifera (1.24) use-values. High UVI of plants depicts that these species are predominantly used in local health care system. The plants Phyllanthus emblica, Morus macruora, Justicia adhatoda and Melia azedarach depicted high values (1.00) of FL and RPL proving that these taxa are commonly used in indigenous medicines by local inhabitants and have better potential for drug discovery by ethnopharmacological analysis. Out of total, 26 species had more than 50% ROP index and Phyllanthus emblica and Flacourtia indica (ROP = 100) followed by Morus macruora (ROP: 97) were used to cure 'hypertension and hyperlipidemia' and 'cough, chest infection', respectively. It was found that 30% of total species were previously reported as medicinal while 70% plants were first time reported to be used in TEMs and authenticated by using of quantitative ethnobotanical tools. Ethnopharmacological potential of indigenous plants was confirmed by RIP and RPL indices which had been used to cure one or more body systems and were promulgated in the local herbal medicine system. The research provides clues to screen these plant species by using latest phytochemical and pharmacological analysis for novel drug discovery. This study will also be useful for conservation of bioculture traditional knowledge of indigenous communities and the most important is to conserve medicinal plants of the study area for future generations.

Indigenous knowledge of medicinal plants from Sudhanoti district (AJK), Pakistan.

ETHNOPHARMACOLOGICAL RELEVANCE: Ethnobotanical study was aimed to document indigenous medicinal plants along with the ethnopharmacological therapeutic values, and to explore the frequencies and relative importance of reported plants. Objective of this study was to trace out the disease prevalence pattern with help of informant consensus factor. MATERIAL AND METHOD: Visual appraisal approach (VAA) and rapid rural appraisal (RRA) methods along with the group meetings and discussions about wide range of topics in an open-ended questionnaire with local people were employed for collection of qualitative ethnomedicinal data. RESULTS AND DISCUSSION: Current study reported 58 medicinal plants belonging to 33 families, where Rosaceae was the predominant family. Berberis lycium dominated among shrubs, which is used to treat liver problems (jaundice). Females and old people were found more allied to the indigenous healthcare system. Conservation threats to valued medicinal plants were detected during course of the study. CONCLUSION: Local communities of the study area are highly dependent on medicinal plants and reported the best therapeutic results for specific disorders. Urgent attention must be paid on conservation of medicinal flora, comprehensive documentation of indigenous medicinal knowledge and pharmacological/phytochemical validation of reported plants for specific diseases.

Relative importance of indigenous medicinal plants from Layyah district, Punjab Province, Pakistan.

ETHNOPHARMACOLOGICAL RELEVANCE: Ethnomedicinal reports are important for the discovery of new crude drugs from reported medicinal plants with high use value. The current study aimed to document the medicinal flora as well as medicinal knowledge of indigenous plants of local communities from Layyah district, Punjab Province, Pakistan. MATERIAL AND METHODS: Rapid appraisal approach (RAA), semi-structured interviews, group meetings with herbalists, landowners and local people having awareness about the medicinal action of plants were employed to collect the data. Use values (UV) and frequency of citation (FC) were calculated to check the relative importance of plants. RESULTS AND DISCUSSIONS: The current ethnomedicinal study reported 125 medicinal plant species diversified from 106 genus belonging to the 43 different families and Poaceae was the predominant family over others with 16 reported medicinal plants. Wild herbaceous plants were the predominant over other life forms while among plant parts, leaves contributed 32% followed by the stem (27%), fruit and flowers (15%). Allium sativum exhibited the highest use vale (UV) 0.92 while the lowest UV was exhibited by Lasiurus scindicus which was 0.15. CONCLUSION: Local communities of district Layyah still have a strong faith on herbal medicines for basic healthcare needs. Indigenous plants having high UV and FC should be subjected to the detailed phytochemical investigations to explore new natural drugs.

An ethnomedicinal survey and documentation of important medicinal folklore food phytonims of flora of Samahni valley, (Azad Kashmir) Pakistan.

Ethnobotanical knowledge is one of the precious cultural heritage parts of an area that involves the interaction between plants and people and foremost among these are the management of plant diversity by indigenous communities and the traditional use of medicinal plants. An ethnobotanical analysis was conducted in order to document the traditional medicinal uses of plants, particularly medicinally important folklore food phytonims of flora of Samahni valley, Azad Kashmir (Pakistan). In the valley, inhabitants use different taxa of flora in two different ways; herbal medicines and food (vegetable and fruits) medicines. The distinctive geographic position and historic demological background of the area keep folk phytotherapy potential of medicinal herbs hitherto alive, which are used in various forms; as regular herbal medicines prescribed by Hakeems (herbal practitioners) and as food (medicines) recepies suggested by elder people. Among these, some herbs are used as single remedy while others depict better curative effects in synergistic mode against various ailments. Some interesting and uncommon findings are as; Sisymbrium irio is used for treatment of measles, asthma; Solanum miniatum to cure urinary calculi, heart pain, rheumatism, Momordica balsamina leaves as wound healer; Allium sativum bulb juice as anti cancer, contraceptive, blood pressure; Boerhavia diffusa roots as anti jaundice, anemia, edema; Capsicum annuum fruit as omen against evil eye and giant, yellow fever; Corriandrum sativum seeds as diuretic, anti spermatogenesis; Raphanus sativus seeds against syphilis; Solanum miniatum fruit for treatment of enlarged spleen and liver; seed's oil of Pisum sativum as anti spermatogenesis; Bauhinia variegata for skin diseases, ulcers; Malva sylvestris for cough, bladder ulcer; Phoenix sylvestris kernel as anti-aging tonic; Phyllanthus emblica for diuretic, anemia, biliousness; Terminalia chebula to cure chronic ulcers, carious teeth pain, heart problems; Veronica anthelmintica for bandage of broken bones and Withania coagulans is used to treat small pox. Many wild plants are eaten green and raw as salad, or in boiled form of soup as blood and intestine cleansing tonics. Moreover, some plants are spiritually recorded as sacred and used as ritual plant for good omens or against the evil eye and removal of giant. About 95 species of 38 families were recorded to be important part of phyto heritage of folk pharmacopoeia of Samahni valley. Among most frequent used families are Papilionaceae 9.47%, Solanaceae and Poaceae 8.42% each, Cucurbitaceae 7.36% and Brassicaceae and Rosaceae 6.31% each. Among the surveyed families used to treat various diseases, Solanaceae is at first rank with 9.74%, Brassicaceae 8.23% and Cucurbitaceae 7.39% subsequently. Most commonly used families with highest percentage of plants used as food medicines are Solanaceae (11.37%), Brassicaceae (8.38%) and Papilionaceae (7.18%) respectively. Most frequent plant parts used are; roots, leaves, seeds and flowers while popular forms of plants uses are decoction, poultice, infusions, soups and raw form as salad. Importance of ethnobotanical inventory constructed from ethnomedicinal uses and folklore phytonims of flora in perspectives of initiative for future phytochemical and pharmacological research on these taxa to develop and discover of new drugs is present and discussed.