ABSTRACT
We studied immunotropic properties of synthetic selenium-organic preparation 2,6-dipyridinium-9-selenabicyclo[3.3.1]nonyl dibromide (974zh). The experimental preparation reduced the cAMP/cGMP ratio, which indicated an increase in proliferative activity of cells of immunocompetent organs (thymus and spleen) in experimental animals. It was shown that 974zh intensified the immune response to Yersinia pestis EV thereby increasing the resistance to the plague agent.
Subject(s)
Immunity, Innate/drug effects , Selenium Compounds/pharmacology , Adjuvants, Immunologic/chemistry , Adjuvants, Immunologic/pharmacology , Adjuvants, Immunologic/therapeutic use , Animals , Combined Modality Therapy , Cyclic AMP/metabolism , Cyclic GMP/metabolism , Female , Male , Mice , Organic Chemicals/chemistry , Organic Chemicals/pharmacology , Organic Chemicals/therapeutic use , Plague/drug therapy , Plague/immunology , Plague/prevention & control , Plague Vaccine/administration & dosage , Selenium/chemistry , Selenium/pharmacology , Selenium/therapeutic use , Selenium Compounds/chemistry , Selenium Compounds/therapeutic use , Spleen/drug effects , Spleen/immunology , Spleen/metabolism , Thymus Gland/drug effects , Thymus Gland/immunology , Thymus Gland/metabolism , Vaccine Potency , Virulence/drug effects , Yersinia pestis/drug effects , Yersinia pestis/immunology , Yersinia pestis/pathogenicityABSTRACT
The results of the study of the preparation of V. cholerae eltor membrane, obtained by the lysis and inactivation of microbial cells with urea and the subsequent differential centrifugation and nuclease treatment. As revealed in this study, the outer membrane preparation, when introduced parenterally and orally to mice, induced pronounced immunity to experimental cholera infection and the production of vibriocidal antibodies in high titers. The treatment of V. cholerae eltor membranes with trypsin led to further increase of the immunogenic potency of the preparation. The protective action of V. cholerae eltor outer membranes considerably exceeded the protective effect of currently used whole-cell eltor vaccine. This opens prospects for using the above-mentioned preparation for the improvement of chemical vaccine as a component ensuring the formation of antibacterial immunity.