ABSTRACT
OBJECTIVE: To establish a quantitative method of simultaneously determination of swertiamarin, gentiopicroside, mangiferin, swertianolin, isoorientin, 1,8-drihydroxy-3-methoxy-xthanone in Swertia from Qinghai province and Sichuan province by HPLC. METHOD: The samples were separated on the column of Kromasil C18 (4. 6 mm x 250 mm, 5 microm) which eluted with methanol and water (content 0.02% phosphoric acid). The ratio of methanol increased from 20% to 80% during 20-50 min, and from 80% to 100% during 50-60 min, with detected wavelength 254 nm, flow rate at 1 mL x min(-1), column temperature 35 degrees C. RESULT: Six compounds were base-isolated, the linear ranges of swertiamarin, gentiopicroside, mangiferin, 4-swertianolin, 5-isoorientin, 1,8-drihydroxy-3-methoxy-xthanone were excellent. CONCLUSION: The method was rapid and precise, and can be use for controlling medicinal materials quality.
Subject(s)
Glucosides/analysis , Iridoids/analysis , Luteolin/analysis , Plants, Medicinal/chemistry , Pyrones/analysis , Swertia/chemistry , Xanthones/analysis , China , Chromatography, High Pressure Liquid/methods , Iridoid Glucosides , Pyrans/analysis , Quality Control , Reproducibility of ResultsABSTRACT
OBJECTIVE: To constitute HPLC fingerprint of the methanol extract from Swertia mussotii grown in Sichuan Province. METHOD: RP-HPLC, methanol and water including 0.02% acid as mobile phase, gradient elution, flow rate 1.0 mL x min(-1), the detection wavelength was 260 nm, temperature was 30 degrees C. RESULT: The RSD values of peak area and retention time of common peaks in precision, repeatability and stability were lower than 5.0%, respectively, similarity was over 0.805 in S. mussotii collected from 10 different habitats. CONCLUSION: All results above exhibit that this method is simple, practicable, and reliable as a standard method in controlling the quality of S. mussotii.