ABSTRACT
Rotavirus enteritis （RVE）， a common infectious disease of the digestive system， is commonly found in infants and young children. Its incidence is high， posing great threats to human health. At present， no specific drugs are available in western medicine and the symptomatic treatments like fluid infusion， anti-diarrhea， correction of electrolyte disorder， protection of gastrointestinal mucosa， and regulation of intestinal microecology have been employed. In addition to the poor therapeutic efficacy， they do not perform well in preventing the onset of RVE and shortening the course of disease. Traditional Chinese medicine （TCM） exerts the therapeutic effect against RVE via multiple targets， without inducing obvious adverse reactions and the cost is low. The specific mechanism of action has yet to be fully explained. Although there are some studies exploring the role of Chinese medicinal monomers in the pathogenesis of RVE， the types involved are still not enough. The effects of Chinese medicinal monomers on autophagy-related pathways fail to be uncovered， which is attributed to the lack of large-scale experimental data and clinical evidence. There are also many problems that cannot be ignored in the related research of Chinese medicinal compounds， taking the superficial exploration and limited scope for instance. Autophagy is a highly conserved biological phenomenon that involves a variety of signaling pathways. Its dysfunction is related to multiple pathological processes. Studies have shown that autophagy plays an important role in the pathogenesis of RVE， especially in the early stage of viral infection. Autophagy induces intestinal mucosal barrier damage， intestinal nerve dysfunction， and immune abnormality， resulting in the occurrence and development of RVE. In recent years， a large number of experimental studies have confirmed that TCM fights against circulatory， respiratory， digestive， and immune system diseases as well as tumors by intervening in autophagy. At the same time， a handful of studies have suggested that Chinese medicinal monomers and compounds regulate autophagy and interfere with viral replication by affecting related signaling pathways， thus playing a positive role in reversing the progression of RVE. However， at present， there are few studies on the regulation of autophagy by TCM in the treatment of RVE， and no systematic elaboration is available. This review aimed to summarize the role of autophagy in the pathogenesis of RVE and its intervention with TCM， in order to provide more theoretical and clinical evidence for the treatment of RVE with TCM.
ABSTRACT
Based on the systematic summary of the results of the fourth general survey of traditional Chinese medicine resources, the cultivation of large varieties of Chinese material medica and the latest research on health industrial development, the novel concepts and scientific connotations of generalized science of Chinese material medica are put forward, and the basic ideas and methods of a new Chinese medicine academic system, the cultivation system of large varieties of Chinese medicinal materials and the application system of the large health industry are constructed. This kind of generalized science of Chinese material medica, rooted in the traditional Chinese culture and the theory of "preventive treatment of disease", can avoid the narrow prospect induced by the increasing specialization and refinement of knowledge of science of Chinese material medica. It will play an important role in the modernization, industrialization, internationalization of traditional Chinese medicine.
Subject(s)
Humans , Drug Industry , Materia Medica , Therapeutic Uses , Medicine, Chinese Traditional , ResearchABSTRACT
Objective To investigate the effect of electroacupuncture on renal CaM-eNOS-NO signal pathway in Dahl salt-sensitive rats with prehypertension.Methods Thirty 7-week-old male Dahl salt-sensitive rats (DS) were allocated,using a random number table,to model,acupuncture and non-meridian-acupoint groups.Ten 7-week-old male Dahl salt-resistant rats (DR) were used as a blank group.The rats were fed in a SPF animal experiment center.An 8％ NaC1 high salt diet was provided after 1-week adaptive feeding.During the period,rat blood pressure was monitored using an intelligent noninvasive sphygmomanometer.A rat model of prehypertension was successfully made if rat blood pressure rose to 120～ 139/80～89 mmHg after 12 days.At that time,a high salt diet was stopped and a normal diet was provided.The blank and model groups received 15-minute regular fixation and no treatment.The acupuncture group received electroacupuncture at bilateral Quchi and Zusanli.The non-meridianacupoint group received electroacupuncture at fixed control points selected between 10 and 15 mm above bilateral iliac crests 20 mm lateral to the midline.Treatment was given once daily,6 times a week,for five consecutive weeks.The left rat kidney was taken.CaM and eNOS mRNA expressions were determined by quantitative fluorescence PCR.eNOS was quantified and located by western blot and immunohistochemical method.NO content was measured by ELISA.Results As compared with the blank group,CaM and eNOS mRNA expressions,eNOS protein content and NO content decreased significantly in the model group (all P＜0.05).As compared with the model group,CaM and eNOS mRNA expressions,eNOS protein content and NO content increased significantly in the acupuncture group (all P＜0.05).As compared with the model group,CaM expression increased significantly (P＜0.05) but eNOS mRNA expression and NO content had no statistically significant differences (P＞0.05) in the non-meridian-acupoint group.Conclusion The renoprotective effect of electroacupuncture at Quchi and Zusanli may be related to upregulating renal CaM-eNOS-NO signal pathway in DS rats with prehypertension.
ABSTRACT
The polysaccharide B3-PS2 was extracted and purified from Herba Scutellariae Barbatae through chromatography of DEAE-cellulose and Sephacryl S-300 column. Average molecular weight of B3-PS2 was about 1,100 kD. It was composed of Glc, Gal and Ara in the ratio of 2.7:2.7:1.0, along with trace of Man, Rha, Fuc and Xyl. B3-PS2 inhibited complement activation on the classic pathways with CH50 value of (0.23 +/- 0.03) mg mL(-1). The targets of B3-PS2 upon the complement system were C1r, C1s, C3 and C4. These results suggested that anti-complementary activity of B3-PS2 was closed to its positive control heparin. It strongly suggested that the polysaccharide B3-PS2 from Herba Scutellariae Barbatae could be a potential candidate in treating those complement-associated diseases.
Subject(s)
Complement Activation , Complement Inactivating Agents , Pharmacology , Drugs, Chinese Herbal , Polysaccharides , Chemistry , Pharmacology , Scutellaria , ChemistryABSTRACT
<p><b>OBJECTIVE</b>To evaluate the effect and safety of transurethral prostatectomy with the bipolar plasmakinetic technique (PKRP) in the treatment of benign prostate hyperplasia (BPH).</p><p><b>METHODS</b>A total of 712 BPH patients underwent transurethral prostatectomy with the bipolar plasmakinetic technique. The patients averaged 70.6 years of age and 52 g (range 35-102 g) in estimated prostate weight preoperatively. Comparative analyses were made on the maximum urine flow rate (Qmax), residual urine volume and scores on IPSS and QOL obtained pre- and post-operatively.</p><p><b>RESULTS</b>The operations lasted 20-120 minutes (mean 51 min), the resected tissues weighed 15-96 g (mean 46 g), and no transurethral resection syndrome (TURS) occurred. The catheters were removed 4 -5 days after surgery. The patients were followed up for 1 -52 months (mean 27.6 mo). Obvious reduction was observed in the average Qmax from 4.7 ml/s preoperatively to 19. 1 ml/s postoperatively, in the mean IPSS score from 26.6 to 5. 8, and in the mean QOL score from 5.4 to 1.7, all with significant differences (P < 0.01).</p><p><b>CONCLUSION</b>Transurethral prostatectomy with the bipolar plasmakinetic technique is a safe and effective means for the treatment of BPH.</p>
Subject(s)
Aged , Aged, 80 and over , Humans , Male , Middle Aged , Prostatic Hyperplasia , General Surgery , Transurethral Resection of Prostate , Methods , Treatment OutcomeABSTRACT
<p><b>OBJECTIVE</b>To evaluate the effect and safety of transurethral prostatectomy with the bipolar plasma kinetic technique (PKRP) in the treatment of benign prostate hyperplasia(BPH).</p><p><b>METHODS</b>Two hundred and ninty-seven BPH patients underwent transurethral prostatectomy with the bipolar plasma kinetic technique. The preoperative estimated weight of the prostate ranged from 35 g to 102 g, averaging 52 g.</p><p><b>RESULTS</b>The operation lasted 40 approximately 65 min, averaging 51 min. The resected tissues weighed 40 approximately 80 g, averaging 46 g. During the operation no transurethral resection (TUR) syndrome occurred. The catheter was removed 4 approximately 5 days after the operation, all with fluent urination. The patients were followed up for 2 approximately 33 months. IPSS decreased from average 31.5 preoperatively to average 6.8 postoperatively (P < 0.001). Average maximum flow-rate (Q(max)) decreased from 6.3 ml/s preoperatively to 18.6 ml/ s postoperatively (P < 0.001). Preoperative average residual urine was 97 ml and reduced to average 9 ml after the operation. Temporary incontinence occurred in 4 cases, perioperative hemorrhage in 2, and urethral stricture in 1.</p><p><b>CONCLUSION</b>Transurethral prostatectomy with the bipolar plasma kinetic technique is a safe and effective means for the treatment of BPH.</p>