Study on content of two sesquiterpenes in Alpinia oxyphylla / 中国中药杂志

<p><b>OBJECTIVE</b>To develop an HPLC method for determining oxyphyllenodiol A (1) and teuhetenone A (2) contained in Alpinia oxyphylla and to compare the contents of the two components contained in medicinal materials and prepared herbal medicines in pieces sold in the market and different fractions.</p><p><b>METHOD</b>HPLC and Waters sunfire C18 column (4. 6 mm x 250 mm, 5 microm) were adopted for gradient elute with the mobile phase of acetonitrile and water. The flow rate was 1.0 mL x min(-1) and the detection wavelength was 250 nm.</p><p><b>RESULT</b>1 and 2 showed a good linear relationship within the range of 0.1296 - 0.8640 microg and 0.1635 - 1.0900 microg respectively, with the average recoveries of 99.08% and 97.80%. Their content ranges were 0.0059% - 0.0149% and 0.0080% - 0.0164% in different samples. The mean value of 1 and 2 were 0.0085% and 0.0104% in the whole fruits, and 0.0137% and 0.0157% in the seeds. They were undetected in the nutshells.</p><p><b>CONCLUSION</b>The method is so precise, accurate and highly reproducible that it can be used to determine the contents of oxyphyllenodiol A and teuhetenone A in A. oxyphylla. The contents of the two components are mainly extracted from the seeds, with certain difference among different samples. There are a higher contents and no significant difference in the salted and raw seeds.</p>

Effects of L.F04, the active fraction of Lycopus lucidus, on erythrocytes rheological property / 中国结合医学杂志

<p><b>OBJECTIVE</b>To study the effects of L.F04, the active fraction of Lycopus lucidus, on erythrocytes rheological property so as to investigate its mechanism in promoting blood circulation and removing blood stasis.</p><p><b>METHOD</b>The effects of L.F04 (used for treatment for 10 days in different dosages) on deformability, aggregation and membrane liquidity of erythrocytes (MLE) as well as whole blood apparent viscosity (eta(b)) were examined on the basis of rat model of blood-stasis syndrome induced by venous injection of high molecular weight dextran.</p><p><b>RESULT</b>As compared with the normal control group, the model group's RBC deformability and MLE were lower, and the aggregation of erythrocytes and eta(b) were higher. Compared with the model group, both L.F04 0.612 g/kg and 0.306 g/kg showed significant effect in improving deformability and inhibiting aggregation of red blood cells (RBC) and reducing blood viscosity. The trend of improving MLE was also shown.</p><p><b>CONCLUSION</b>L.F04 could significantly improve the abnormal rheological property of erythrocytes.</p>

Research on the technology of soaking under pressure and cutting process of Radix Curcumae / 中国中药杂志

<p><b>OBJECTIVE</b>To determine the best processing technology parameters.</p><p><b>METHOD</b>The changes of the weights, volatile constituents, total extract quantities by MeOH or CHCl3 and curcumenol contents of the roots of Curcuma wenyujin and C. kwangsiensis roots before and after water soaking were determined, so as to determine the quality control index for the process of preparing Yujin slices. The shortest soaking and moistening time and the appropriate drying temperature were studied.</p><p><b>RESULT AND CONCLUSION</b>(1) The weight was decreased and the quantities of volatile constituents were changed during water soaking process. But the total extracts quantities and the curcumenol contents didn't change obviously. So the quality control index was decided as soaking as short as possible. (2) The soaking time could be decreased by using high pressure. The soaking time was related with the short diameter of the cross section of the root. (3) The soaking under pressure technology is that soaking on -0.095 MPa for 0.5 h, then on 0.14 MPa for 10-16 h, and moistening for 36-48 h, cutting drying in room temperature or less than 40 degrees C.</p>

Chemical constituents from the seeds of Annona squamosa / 药学学报

<p><b>AIM</b>To study the antitumor active constituents of the seeds from Annona squamosa L. (Annonaceae).</p><p><b>METHODS</b>Various chromatographic techniques were used to isolate and purify the constituents. Their physico-chemical properties and spectral data were determined to elucidate the structures.</p><p><b>RESULTS</b>Eleven compounds were isolated and identified as annonaceous acetogenins: squamocenin (1), annotemoyin-2 (2), reticulatain-2 (3), squamocin-I (4), squamocin-B (5), squamocin (6), motrilin (7), squamostatin-D (8), squamostatin-E (9), cherimolin-1 (10), cherimolin-2 (11) from the ethyl alcohol extract of A. squamosa L.</p><p><b>CONCLUSION</b>Squamocenin (1) is a new acetogenin. Annotemoyin-2 (2) and reticulatain-2 (3) were isolated from this plant for the first time.</p>

Two flavone glycosides from Chinese traditional medicine Amomum villosum / 中国中药杂志

<p><b>OBJECTIVE</b>To study the water soluble constituents from Amomum villosum.</p><p><b>METHOD</b>The constituents were separated and purified with chromatographic methods, identified by NMR, MS, UV and IR.</p><p><b>RESULT</b>Two quercetin glycosides: quercitrin (quercetin-3-O-alpha-L-rhamnoside I) and isoquercitrin (quercetin-3-O-beta-D-glucoside II) were isolated and identified.</p><p><b>CONCLUSION</b>I and II were isolated for the first time from A. villosum.</p>