ABSTRACT
Polydatin is extracted from a traditional Chinese herbal medicine, Polygonum cuspidatum, and has a special effect in shock treatment. The aim of this study is to explain the cellular and molecular basis of Polydatin in shock treatment. The fluorescent probe techniques, patch clamp method, and cellular flow chamber were used to test intracellular variables of vascular smooth muscle cells (VSMC), myocardial cells (MC), endothelial cells (EC), and white blood cell (WBC). It was shown that Polydatin could inhibit ICAM-1 expression in EC stimulated by lipopolysaccharide (LPS), attenuate WBC-EC adhesion, increase [Ca2+]i in MC with enhancement of MC contraction extent, activate KATP channels of VSMC, and decrease pHi value and [Ca2+]i of VSMC in shock. The study suggests that Polydatin has multiple effects on VSMC, MC, WBC and EC, which are related to the enhancement of heart function and improvement of microcirculatory perfusion in shock.
Subject(s)
Fallopia japonica/chemistry , Glucosides/pharmacology , Microcirculation/drug effects , Phytotherapy , Shock, Hemorrhagic/drug therapy , Stilbenes/pharmacology , Animals , Calcium Channel Blockers/pharmacology , Calcium Signaling/drug effects , Cell Adhesion/drug effects , Cells, Cultured/drug effects , Drug Evaluation, Preclinical , Endothelial Cells/drug effects , Endothelium, Vascular/cytology , Endothelium, Vascular/drug effects , Glucosides/therapeutic use , Humans , Intercellular Adhesion Molecule-1/biosynthesis , Intercellular Adhesion Molecule-1/genetics , Leukocytes/drug effects , Leukocytes/physiology , Muscle, Smooth, Vascular/cytology , Muscle, Smooth, Vascular/drug effects , Myocardium/cytology , Patch-Clamp Techniques , Plants, Medicinal/chemistry , Potassium Channels/drug effects , Rats , Shock, Septic/drug therapy , Shock, Septic/physiopathology , Stilbenes/therapeutic use , Verapamil/pharmacologyABSTRACT
OBJECTIVE: To investigate the effect of Chinese traditional medicine complex dribbing-pill of Xue Shuan Tong on thrombus formation in vivo and thrombolysis in vitro of rats. METHODS: Thrombus and thrombolysis were evaluated by carotid artery-jugular vein circuit in vivo and constant temperature natural lysis in vitro respectively. RESULTS: Complex dribbing-pill of Xue Shuan Tong could significantly inhibit thrombus formation in vivo. Coagulated blood could be apparently dissolved by complex dribbing-pill of Xue Shuan Tong. CONCLUSION: Complex dribbing-pill of Xue Shuan Tong could significantly inhibit the thrombus and increase thrombolysis.