Determination on Tree Species Selection for Lingzhi or Reishi Medicinal Mushroom Ganoderma lucidum (Agaricomycetes) Cultivation by Fourier Transform Infrared and Two-Dimensional Infrared Correlation Spectroscopy.

As a wood-degrading Agaricomycetes mushroom, Ganoderma lucidum can be cultivated on broad-leaf hardwoods. Generally, producers care about the yield, but not the quality of G. lucidum cultivated by different tree species. In this study, five broad-leaf hardwood tree species-Quercus variabilis Bl. (Qv), Castanea mollissima Bl. (Cm), Liquidambar formosana Hance (Lf), Dalbergia hupeana Hance (Dh), and Platycarya strobilacea Sieb. et Zucc. (Ps)-were selected for cultivating of G. lucidum. The chemical compositions of G. lucidum fruiting bodies produced by these tree species were determined by Fourier transform infrared and two-dimensional infrared correlation spectroscopy in order to select the most suitable tree species for cultivation. The overall spectra showed less discrimination of each peak variation detected and properly kept most of the primary metabolites. The second derivative unfolded the stagnation of the first spectrum and more base peaks were detected especially in the range of the first two sections. The protein content contained in G. lucidum cultivated on Ps was 92%, like that on Dh. On the other hand, only 27% similarity was determined in G. lucidum cultivated on Ps and Qv. Therefore, the correlation of this range for the protein content can help in tree species selection. The active sequence of 2DIR spectral could be determined by the active bonding of the component reacted to the perturbation. The result could provide a scientific basis for the selection of tree species and the comprehensive utilization of broad-leaf tree resources on G. lucidum cultivation.

Fractionation and Biological Activities of Water-Soluble Polysaccharides from Sclerotium of Tiger Milk Medicinal Mushroom, Lignosus rhinocerotis (Agaricomycetes).

This study investigated antioxidant and anti-inflammatory properties, and the direct cytotoxic effect of Lignosus rhinocerotis fractions, especially the polysaccharide fraction, on nasopharyngeal carcinoma cells. L. rhinocerotis crude extract was obtained through hot water extraction. The precipitate saturated with 30% ammonium sulfate was purified with ion-exchanged chromatography. Gel permeation chromatography multiangle laser light scattering analysis equipped with light scattering and UV signals revealed two district groups of polymers. A total of four peaks were observed in the total carbohydrate test. Fraction C, which was the second region of the second peak eluted with 0.3 M NaOH, showed the highest integrated molecular weight, whereas fraction E had the lowest integrated molecular weight of 19,790 Da. Fraction A contained the highest ß-D-glucan content. Enzymatic analysis showed that most of the polysaccharide fractions contained ß-1-3 and ß-1-6 skeletal backbones. The peak eluted with 0.6 M NaOH was separated in fraction D (flask 89-92) and fraction E (93-96). The results showed that fraction E expressed higher antioxidant activities than fraction D whereas fraction D expressed higher chelating activity than fraction E. The extract saturated with 30% ammonium sulfate exhibited higher reducing power than the extract saturated with 100% ammonium sulfate. Fractions D and E significantly inhibited the secretion of tumor necrosis factor-α in lipopolysaccharide-stimulated RAW 264.7 macrophages in a dose-dependent manner. There was no apparent difference in the viability of cells exposed or unexposed to L. rhinocerotis fractions.