ABSTRACT
OBJECTIVE: The chemical components of essential oil from Anaphalis aureopunctata were analyzed by GC-MS. METHODS: Essential oil was extracted by stem distillation (SD). The chemical components of essential oil were analyzed by GC-MS. RESULTS: The main components in the oil were Eudesma-4(14), 11-diene, alpha-Bisabolol,6,10,14-trimethyl- 2-Pentadecanone,Caryophyllene oxide,alpha-Selinene and so on. CONCLUSION: This is the first time to adopt GC-MS to analyze the chemical components of volatile oil of Anaphalis au-reopunctata, and this study can provide science basis for further research development of Anaphalis aureopunctata.
Subject(s)
Asteraceae/chemistry , Gas Chromatography-Mass Spectrometry/methods , Oils, Volatile/chemistry , Plants, Medicinal/chemistry , Sesquiterpenes/analysis , Esters/analysis , Fatty Acids, Unsaturated/analysis , Oils, Volatile/isolation & purification , Sesquiterpenes, Eudesmane , Tetrahydronaphthalenes/analysisABSTRACT
OBJECTIVE: To study chemical constituents of Dendranthema indicum var. aromaticum. METHODS: The constituents were separated and purified by column chromatography with silica gel and Semi-preparative HPLC. Their structures were identified on the basis of physical-chemical properties and spectral data. RESULTS: Seven compounds were isolated and identified as acacetin, apignein, acacetin-7-O-beta-D-glucopy ranoside, apignein-7-O-beta-D-glucopy ranosids, luteolin, beta-sitosterol and daucosterol. CONCLUSION: Apigenin, acacetin-7-O-beta-D-glucopy ranoside and apignein-7-O-beta-D-glucopy ranoside are obtained from the plant for the first time.
Subject(s)
Apigenin/isolation & purification , Asteraceae/chemistry , Luteolin/isolation & purification , Plants, Medicinal/chemistry , Apigenin/chemistry , Chromatography, Thin Layer , Flavonoids/chemistry , Flavonoids/isolation & purification , Flowers/chemistry , Glucosides/chemistry , Glucosides/isolation & purification , Luteolin/chemistry , Sitosterols/chemistry , Sitosterols/isolation & purificationABSTRACT
OBJECTIVE: The chemical components of essential oil from Magnolia biondii were analyzed by GC-MS. METHODS: Essential oil was extracted by steam distillation (SD). The chemical components of essential oil were analyzed by GC-MS. RESULTS: The chemical components in the oil were qualitatively and quantitatively analyzed by GC-MS. 63 components were separated and 50 components were identified. The main components were Eucalyptol (28.92%), P-pinene (12.39%), alpha-Terpineol (8.28%). CONCLUSION: This is the first time to adopt GC-MS to analyze the chemical components of volatile oil of Magnolia biondii, and this study can provide science basis for further research development of Magnolia biondii.
Subject(s)
Magnolia/chemistry , Monoterpenes/analysis , Oils, Volatile/isolation & purification , Plants, Medicinal/chemistry , Bicyclic Monoterpenes , Bridged Bicyclo Compounds/analysis , Bridged Bicyclo Compounds/chemistry , Cyclohexane Monoterpenes , Cyclohexanols/analysis , Cyclohexanols/chemistry , Cyclohexenes/analysis , Cyclohexenes/chemistry , Eucalyptol , Flowers/chemistry , Gas Chromatography-Mass Spectrometry , Monoterpenes/chemistry , Oils, Volatile/chemistry , Steam , Technology, Pharmaceutical/methodsABSTRACT
OBJECTIVE: To establish the HPLC fingerprint spectrum of flavonoids from Gynostemma pentaphyllum. METHODS: The HPLC conditions with TURNER Kromasil C18 column (250 x 4. 6 mm, 5 microm) was used, the mobile phase consisted of 0.4% phosphoric acid-methanol-acetonitrile with gradient elution at flow rate of 0.5 ml/min and the detection wavelength was set at 360nm. RESULTS: Ten common characteristic peaks were taken as fingerprint peaks the presision and the retest are in accordance with the requirement. CONCLUSION: The method is stable, reliable, precision and provides a scientific basis for the quality standard of Gynostemma pentaphyllum.