ABSTRACT
OBJECTIVE: To investigate the location and anatomical structure of "Shaochong"(HT9), "Shaofu"(HT8), "Shenmen"(HT7), "Lingdao"(HT4) and "Shaohai"(HT3) in the rabbit's forelimb. METHODS: Sixteen rabbits (half male and half female) were used in the present study. By referring to the national standards on the location of acupoints in the human body and the literature about the location of acupoints in the rabbit, and by using the method of comparative anatomy, the location and needling operation of the Five-shu acupoints of Shaoyin Heart Meridian on the rabbit's forelimb were defined, and these acupoints were needled and CT three-dimensional reconstruction were conducted. Then, the rabbits were killed, and intravascular perfusion was performed, followed by inserting acupuncture needles into these five acupoints for observing the anatomical relationship between the inserted acupuncture needle and the structure of surrounding tissues. RESULTS: HT9 is located at the medial side of the little finger of forelimb, about 1 mm beside the nail root, and is adjacent to the superficial flexor tendon of the finger, the dorsal branches of the proper palmar digital artery and vein, and the endings of dorsal branch of palmar digital proper nerve of the ulnar nerve on the fifth finger side. HT8 is located at the palm side of the forelimb, horizontally parallel to the proximal end of the 5th metacarpophalangeal joint and between the 4th and 5th metacarpal bones, and is adjacent to the lumbricalis, the 4th and 5th interossei, and common palmar digital artery and vein and the palmar digital proper nerve of the ulnar nerve. HT7 is located at the medial margin of the extensor carpal tendon on the ulnar side, between the distal end of the ulna and the ulnar carpal bone, and is adjacent to the tendons of flexor carpi ulnaris and extensor carpi ulnaris, ulnar artery, ulnar vein and ulnar nerve. HT4 is located at the medial border of the ulnar flexor tendon, about 1.5 cun superior to HT7, and is adjacent to extensor carpi ulnaris, flexor carpi ulnaris, flexor digitorum superficialis, flexor digitorum profundus, ulnar artery, vein and ulnar nerve. HT3 is located at the depression, medial to the condyle of humerus when the elbow is bent at 90°, its neighbor structure is composed of pronator teres, biceps brachii, brachial artery and vein, radial collateral artery, radial collateral vein, medial antebrachial cutaneous nerve and median nerve. CONCLUSION: In the rabbit, there is a close relationship between HT9, HT8, HT7, HT4 and HT3 regions and brachial vascular and its branches, cephalic vein and its branches, medial antebrachial cutaneous nerve, median nerve and ulnar nerve, which is the morphological basis of the Five-shu acupoints of Shaoyin Heart Meridian for treating some related clinical disorders.
Subject(s)
Meridians , Animals , Rabbits , Male , Female , Humans , Acupuncture Points , Imaging, Three-Dimensional , Forelimb/diagnostic imaging , Forelimb/anatomy & histology , Tomography, X-Ray ComputedABSTRACT
Mitochondria, as the powerhouse of most cells, are not only responsible for the generation of adenosine triphosphate (ATP) but also play a decisive role in the regulation of apoptotic cell death, especially of cancer cells. Safe potential delivery systems which can achieve organelle-targeted therapy are urgently required. In this study, for effective pancreatic cancer therapy, a novel mitochondria-targeted and ROS-triggered drug delivery nanoplatform was developed from the TPP-TK-CPI-613 (TTCI) prodrug, in which the ROS-cleave thioketal functions as a linker connecting mitochondrial targeting ligand TPP and anti-mitochondrial metabolism agent CPI-613. DSPE-PEG2000 was added as an assistant component to increase accumulation in the tumor via the EPR effect. This new nanoplatform showed effective mitochondrial targeting, ROS-cleaving capability, and robust therapeutic performances. With active mitochondrial targeting, the formulated nanoparticles (TTCI NPs) demonstrate much higher accumulation in mitochondria, facilitating the targeted delivery of CPI-613 to its acting site. The results of in vitro antitumor activity and cell apoptosis revealed that the IC50 values of TTCI NPs in three types of pancreatic cancer cells were around 20~30 µM, which was far lower than those of CPI-613 (200 µM); 50 µM TTCI NPs showed an increase in apoptosis of up to 97.3% in BxPC3 cells. Therefore, this mitochondria-targeted prodrug nanoparticle platform provides a potential strategy for developing safe, targeting and efficient drug delivery systems for pancreatic cancer therapy.
ABSTRACT
OBJECTIVE: To observe the anatomic structure of the Five Shu-acupoints: "Shaoze" (SI 1) ,"Qiangu" (SI 2), "Houxi" (SI 3),"Yanggu" (SI 5) and "Xiaohai" (SI 8) regions of the Taiyang Meridian in the rabbit's forelimb. METHODS: Thirty rabbits (half male and half female) were used in the present study. The Five Shu-acupoints regions were located first based on the atlas of rabbits, stimulated by needling and confirmed later by using an electronic acupoint detector. Under anesthesia, the rabbit was perfused with warm normal saline via the common carotid artery and the internal jugular vein, followed by arterial perfusion of dental base acrylic resin powder(30 g), dibutylphthalate(6 mL), red couring agent liquid for denture acrylic and acetoacetate (2 mL), respectively; and venous perfusion of 30% gelatin (filtered) and black ink (filtered) and formaldehyde (8%). After fixing in 8% formaldehyde for 10 days, the rabbit's forelimb containing the aforementioned Five Shu-acupoints were carefully dissected layer by layer, followed by observing the local anatomic structure under microscope. RESULTS: The superficial layers of these acupoint regions mainly contained the basilic vein and its branches, and the superficial branch of the ulnar nerve. The deep layers chiefly comprised of the ulnar artery, the ulnar vein and their branches, and the ulnar nerve. CONCLUSION: In "Shaoze"(SI 1 ), "Qiangu" (SI 2), "Houxi" (SI 3), "Yanggu" (SI 5) and "Xiaohai" (SI 8) acupoint regions, the ulnar artery, basilic vein, ulnar vein and their branches, the ulnar nerve and its superficial branches are found, which constitute the morphological basis of the five acupoints of the Hand-Taiyang Meridian for treating some related clinical disorders.