ABSTRACT
Three new meroterpenoid derivatives, furanocochlioquinol (1) and furanocochlioquinone (2), as well as nectrianolin D (3), together with two known biogenetically related compounds 4 and 5 were isolated from a mixed culture of two mangrove-derived fungi, Clonostachys rosea B5-2 and Nectria pseudotrichia B69-1. The structures of 1-3 were deduced based on the interpretation of HRMS and NMR data. Compounds 1-5 exhibited cytotoxicity against human promyelocytic leukemia (HL60) cells with IC50 values ranging from 0.47 to 10.16 µM.
Subject(s)
Antineoplastic Agents/pharmacology , Hypocreales/chemistry , Nectria/chemistry , Rhizophoraceae/microbiology , Terpenes/pharmacology , Antineoplastic Agents/isolation & purification , Biological Products/isolation & purification , Biological Products/pharmacology , Coculture Techniques , Endophytes/chemistry , HL-60 Cells , Humans , Indonesia , Molecular Structure , Terpenes/isolation & purificationABSTRACT
Five polyketides, paralactonic acids A-E (1-5) were isolated from Paraconiothyrium sp. SW-B-1, an endophytic fungus isolated from the seaweed, Chondrus ocellatus Holmes. Their structures were determined by various spectroscopic methods, predominantly by 2D NMR spectroscopic analyses. The absolute configurations of compounds 1-5 were determined by comparison of the experimental and calculated ECD spectra. Compound 5 showed moderate antibacterial activity and restored the growth of a mutant yeast strain inhibited by hyperactivated Ca2+-signaling.
Subject(s)
Anti-Bacterial Agents/chemistry , Ascomycota/chemistry , Chondrus/microbiology , Polyketides/chemistry , Anti-Bacterial Agents/isolation & purification , Japan , Molecular Structure , Polyketides/isolation & purification , Seaweed/microbiologyABSTRACT
A new limonoid, pentandricine (1), along with three known limonoids, ceramicine B (2), 6-de(acetyloxy)-23-oxochisocheton (3), 6-de(acetyloxy)-23-oxo-7-O-deacetylchisocheton (4), have been isolated from the stembark of Chisocheton pentandrus. The chemical structures of the new compound were elucidated on the basis of spectroscopic evidence. All of the compounds were tested for their cytotoxic effects against MCF-7 breast cancer cells. Compounds 1-4 showed weak and no cytotoxicity against MCF-7 breast cancer cells with IC50 values of 369.84, 150.86, 208.93 and 120.09 µM, respectively.