Acute Effects of Short-Term Massage Procedures on Neuromechanical Contractile Properties of Rectus Femoris Muscle.

Background and Objectives: In many sports, maintaining muscle work at an optimal level despite fatigue is crucial. Therefore, it is essential to discover the most efficient way of recovery. This study aimed to evaluate and compare the acute effects of four different recovery methods on muscle neuromechanical properties. Materials and Methods: The research was conducted using a randomized, quasi-experimental, repeated-measures design. Fourteen healthy and active male students of the Faculty of Sport and Physical Education (age 25.1 ± 3.9 years) were included in this study. The tensiomyography was used to evaluate muscle responses after four different types of short-term recovery methods (passive rest, percussive mechanical, vibro-mechanical, and manual massage) on the rectus femoris muscle on four occasions: baseline, post fatigue, post recovery and prolonged recovery. Results: The ANOVA revealed that muscle fatigue decreased maximal vertical muscle displacement (Dm) and muscle contraction time (Tc) in post fatigue compared to the baseline. The most important finding shows that only the vibro-mechanical massage resulted in an increase in Tc in the prolonged recovery compared to the post fatigue (p = 0.028), whereas only manual massage showed no differences in Dm from the baseline in post-recovery (p = 0.148). Moreover, both manual and vibro-mechanical massages increased Dm and Tc in prolonged recovery, indicating no differences from the baseline (all p > 0.05), thus showing signs of muscle recovery. Percussion mechanical massage and passive rest did not show indices of muscle recovery. Conclusions: Manual massage could induce immediate positive changes in Dm by reducing muscle stiffness. In addition, vibro-mechanical and manual massage improved muscle tissue by rapidly returning Dm and Tc values to baseline at prolonged recovery measurement (5 min after the fatigue protocol). These findings can benefit sports practitioners, and physical therapists in developing the best recovery method after muscle fatigue.

Immunomodulatory Activity of Punicalagin, Punicalin, and Ellagic Acid Differs from the Effect of Pomegranate Peel Extract.

BACKGROUND: Our recent study has shown that pomegranate peel extract (PEx) showed significant immunomodulatory activity, which might be caused by ellagitannins. The aim of this work was to test the hypothesis that ellagitannin components act synergistically in the modulation of cytokine production. METHODS: Human peripheral blood mononuclear cells (PBMCs) from healthy donors were stimulated with phytohemagglutinin and treated with different concentrations of PEx or punicalagin (PG), punicalin (PN), and ellagic acid (EA), alone or with their combinations. Cytotoxicity, cell proliferation, and cytokine production were determined. RESULTS: Non-cytotoxic concentrations of all compounds significantly inhibited cell proliferation. IC50 values (µg/mL) were: EA (7.56), PG (38.52), PEx (49.05), and PN (69.95). PEx and all ellagitannins inhibited the levels of TNF-α, IL-6, and IL-8, dose-dependently, and their combinations acted synergistically. PEx and all ellagitannins inhibited Th1 and Th17 responses, whereas the lower concentrations of PEx stimulated the production of IL-10, a Treg cytokine, as did lower concentrations of EA. However, neither component of ellagitannins increased Th2 response, as was observed with PEx. CONCLUSIONS: The combination of PG, PN, and EA potentiated the anti-inflammatory response without any significant synergistic down-modulatory effect on T-cell cytokines. The increased production of IL-10 observed with PEx could be attributable to EA, but the examined ellagitannins are not associated with the stimulatory effect of PEx on Th2 response.

Immunomodulatory Effects of Extract of Lingzhi or Reishi Medicinal Mushroom Ganoderma lucidum (Agaricomycetes) Basidiocarps Cultivated on Alternative Substrate.

Ganoderma lucidum is a medicinal mushroom exhibiting numerous health benefits primarily based on strong immunostimulatory effects. The study aimed to investigate if there were differences in effects of extracts of commercially (GC) and alternatively (wheat straw) (GA) cultivated G. lucidum basidiocarps on properties of peritoneal macrophages (PM) and monocyte-derived dendritic cells (MoDCs). Differences in immunomodulatory effects of GC/GA extracts were studied. The viability of treated PMs, their adhesive and phagocytic capability, and their capacity to produce reactive oxygen species (ROS) and NO were tested. Immature MoDCs generated from human monocytes were treated with poly I:C (10.0 µg/ml) and loxoribine (34.0 µg/ml), a selective TLR3 and TLR7 agonists, respectively, and with/without GC/GA extract (100.0 µg/ml). The effect of each combination on phenotypic properties, cytokines production by MoDCs, and their proliferation and Th polarizing capacity was studied. GA extract stimulated the metabolic and phagocytic activity of PMs, their adhesion capability, and ability to produce ROS and NO more strongly compared to GC. Both tested extracts significantly increased allostimulatory and Th1 polarization capacity of simultaneous TLR3 and TLR7-activated MoDCs, but GA extract was more effective. The extract of alternatively cultivated G. lucidum basidiocarps increased production of ROS and NO by TLR4 stimulated PMs and upregulated production of certain cytokines as well as allostimulatory and Th1 polarization capacity of MoDCs. GA extract could be a potent immunostimulatory agent for activation of MoDCs with the simultaneous engagement of TLRs, which seems to be a promising strategy for the preparation of DC-based anti-tumor vaccines.

Rethinking the Process of Animal Mummification in Ancient Egypt: Molecular Characterization of Embalming Material and the Use of Brassicaceae Seed Oil in the Mummification of Gazelle Mummies from Kom Mereh, Egypt.

The study of animal mummification in ancient Egypt has recently received increasing attention from a number of modern scholars given the fact that this part of ancient Egyptian funerary and religious history is a practice yet to be fully understood. In this study, nine samples of embalming matter were extracted from six gazelle mummies from the archaeological site of Kom Mereh (modern village of Komir), dated to the Roman period of dominance in ancient Egypt. All samples were analyzed for the presence of inorganic and organic matter applying a multi-analytical approach based on Fourier transform infrared spectroscopy (FT-IR) and gas chromatography-mass spectrometry (GC-MS). Furthermore, in order to identify more specific compounds such as bitumen and beeswax in studied balms, each sample was subjected to a solid phase extraction (SPE) and saponification separation process, respectively. The results of this study revealed that the majority of the analyzed embalming substances sampled from six gazelle mummies from Kom Mereh were complex mixtures of plant oils, animal fats, conifer resin, and beeswax. In this regard, this study was able to report a practice until now unmentioned in the scientific literature, namely, the use of cruciferous oil, derived from seeds of Brassicaceae plants, in animal mummification.

Validity of a Novel Specific Wrestling Fitness Test.

ABSTRACT: Markovic, M, Kukic, F, Dopsaj, M, Kasum, G, Toskic, L, and Zaric, I. Validity of a novel specific wrestling fitness test. J Strength Cond Res 35(12S): S51-S57, 2021-The specific wrestling fitness test (SWFT) is a novel test aiming to estimate the level of physical preparedness of wrestlers; therefore, it should posses an acceptable level of validity. The aim of this study was to investigate an internal, external, and construct validity of SWFT. The sample consisted of 15 national level male wrestlers (age = 22.6 ± 2.3 years, body mass [BM] = 83.3 ± 6.5 kg, and BM index [BMI] = 25.36 ± 1.2 kg·m-2). They performed the SWFT, specific judo fitness test (SJFT), and specific wrestling performance test (SWPT), each test on a separate day. For each test, performance was evaluated in absolute measure as total number of throws at the end of the test (TnThrows) and relative measure as TnThrows/BM and TnThrows/BMI. Heart rate at the end of the test (HR0min) and 1 minute into recovery (HR1min) was used as a measure of cardiovascular functionality, whereas specific judo fitness index (SJFIndex) was used as an indicator of cardiovascular functionality relative to given performance. A correlation and multiple linear regression analyses were used to investigate the internal, external, and construct validity of SWFT. The SWFT_TnThrows/BM had the highest internal validity relative to SJFT_TnThrows/BM (R2 = 0.722, p < 0.001) and the highest external validity relative to SWPT_TnThrows/BM (r = 0.846, p < 0.001). SWFT_TnThrows/BM predicted SWPT_TnThrows/BM with a large coefficient of determination (R2 = 0.818, p < 0.001). SWFT_TnThrows/BM is valid and easily attainable predictor of wrestlers' specific physical preparedness and as such is of high practical value.

Discrimination of different body structure indexes of elite athletes in combat sports measured by multi frequency bioimpedance method / Discriminación de diferentes índices de estructura corporal de atletas de elite en deportes de combate medidos por el método de bioimpedancia de múltiples frecuencias

In order to achieve high results in sports, it is necessary to provide an adequate status of an athlete in according to various factors, among which the body structure is one of greater importance. The aim of this research is to define the most discriminated indicators of body structure indexes, thanks to which a specific change of physical structure can be observed according to a branch of sport and type of combat sport. Variables in the research were: body height, body mass, body mass index, free fat mass index, fat mass index, protein mass index, skeletal muscle mass index, percent of body fat, percent of skeletal muscle mass and protein fat index. The measuring of the body composition is realized by using multichannel segmental bioimpedance with InBody 720 apparatus, applied on the total of 112 male high level senior-age athlete members of different national team's member of Republic of Serbia (62 judokas, 29 Greco-Roman style wrestlers and 21 karatekas). The discriminative analysis has shown that subsamples of the athletes statistically differ in morphology on Wilks' lambda level (0.435, p = 0.000), and that following variables have the highest discrimination compared to athlete respondents in sport function: percent of skeletal mass (0.34), percent of body fat (0.28) and protein fat index (0.26), as the first isolated factor (p = 0.000) by which is explained 86.3 %, that is body weight (0.74), skeletal muscle index (0.73), protein mass index (0.72), body mass index (0.72) and free fat mass index (0.70), as the second factor (p = 0.030) by which 13.7 % variability of measured body structure space is explained. By establishing body structure models of judokas, wrestlers and karatekas for variables examined and by using modern technological method of measuring (InBody 720, 2008) we got new information that characterize specificity of the sport, which all contribute to spreading and improving already existing knowledge in the sports sciences and sport body morphology area.

Para obtener logros deportivos de alto nivel, es necesario conseguir el estatus adecuado dependiendo de varios factores, entre los cuales destaca el factor morfológico. El objetivo de este estudio fue definir los indicadores más discriminantes de la composición corporal o índices morfológicos, por lo cual se puedan monitorear los cambios específicos en la estructura del cuerpo en relación con la rama del deporte y el tipo de deportes de combate. Las variables del estudio fueron: altura del cuerpo, masa corporal, índice de masa corporal, índice de masa grasa, índice de proteína masa, índice de masa del músculo esquelético, porcentaje de grasa corporal, porcentaje de masa del músculo esquelético y el índice grasa proteína. La medición de la composición corporal fue realizada utilizando la bioimpedancia segmentaria multicanal con Inbod café 720 sobre una muestra de 112 deportistas de sexo masculino de categoría sénior, miembros nacionales seleccionados de la República de Serbia (62 practicantes de judo, 29 practicantes de lucha grecorromana y 21 practicantes de karate). El análisis discriminativo demostó que las submuestras de los atletas en general difieren significativamente en la morfología a nivel de lambda de Wilks ­ 0.435, (p = 0.000), y que las siguientes variables tienen la mayor discriminación en relación con la prueba de los atletas en la función del deporte: porcentaje de la masa esquelética (0.34), porcentaje de grasa corporal (0,28) y el índice de grasa proteína (0.26), como el primer factor aislado (p = 0.000), lo que explica 86,3 %, respectivamente la masa corporal (0.74), el índice de músculo esquelético (0,73), el índice de proteína de masa (0,72), índice de masa corporal (0,72), e índice de masa libre de grasa (0,70), como el segundo factor (p = 0,030), lo que explica 13,7 % de las mediciones espaciales de variabilidad. Al definir los módulos morfológicos de los practicantes de judo, karate y lucha grecorromana para las variables investigadas y usando el método tecnológico de medida más nuevo (InBody 720), hemos obtenido nuevas informaciones que caracterizan las especificidades de los deportes, lo que contribuye a la ampliación y el desarrollo de los conocimientos anteriores sobre dicho asunto.